Western Blot: Proteasome 19S 10B Antibody [NB100-1483] - Staining of HeLa (A), U251 (B), KNRK (C) with antibody at 1 ug/mL and NIH3T3 (D) cell lysate with antibody at 0.3 ug/mL (35 ug protein in RIPA buffer). Detected ...read more
Immunocytochemistry/ Immunofluorescence: Proteasome 19S 10B Antibody [NB100-1483] - HepG2 cells were treated with DMSO, 2uM RA190 for or 25uM MG132 for 30min. The cells were fixed, permeabilized and stained for ...read more
Flow Cytometry: Proteasome 19S 10B Antibody [NB100-1483] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa ...read more
Immunocytochemistry/ Immunofluorescence: Proteasome 19S 10B Antibody [NB100-1483] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) ...read more
Western blot: Approx 100 kDa band observed in lysates of cell line A431 (calculated MW of 100 kDa according to NP_002799.3). Use in Immunocytochemistry/immunofluorescence and use in Proximity ligation assay reported in scientific literature (PMID: 26150391).
tumor necrosis factor receptor-associated protein 2
Tumor necrosis factor type 1 receptor-associated protein 2
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. 26S S2 is one of the non-ATPase subunits of the 19S regulator lid. In addition to participation in proteasome function, this subunit may also participate in the TNF signalling pathway since it interacts with the tumor necrosis factor type 1 receptor. A pseudogene has been identified on chromosome 1
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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