Gemin 4 Antibody

Images

 
Western Blot: Gemin 4 Antibody [NB110-40591] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NB110-40591 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) ...read more
Immunohistochemistry: Gemin 4 Antibody [NB110-40591] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Gemin4 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: Gemin 4 Antibody [NB110-40591] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-Gemin4 used at a dilution of 1:250.
Immunohistochemistry-Paraffin: Gemin 4 Antibody [NB110-40591] - Human breast carcinoma. Antibody used at a dilution of 1:200.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Gemin 4 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1010 and the C-terminus (residue 1058) fo human Gem (nuclear organelle) Associated Protein 4 using the numbering given in entry NP_056536.1 (GeneID 50628).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GEMIN4
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
Theoretical MW
120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Gemin 4 Antibody

  • component of gems 4
  • DKFZp434B131
  • DKFZP434D174
  • gem (nuclear organelle) associated protein 4
  • gemin-4
  • HC56
  • HCAP1
  • HCC-associated protein 1
  • HHRF-1
  • p97DKFZp434D174

Background

The product of this gene is part of a large complex localized to the cytoplasm, nucleoli, and to discrete nuclear bodies called Gemini bodies (gems). The complex functions in spliceosomal snRNP assembly in the cytoplasm, and regenerates spliceosomes required for pre-mRNA splicing in the nucleus. The encoded protein directly interacts with a DEAD box protein and several spliceosome core proteins. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol GEMIN4
Entrez
Uniprot