Western Blot: Gemin 4 Antibody [NB110-40591] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NB110-40591 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) ...read more
Immunohistochemistry-Paraffin: Gemin 4 Antibody [NB110-40591] - Human breast carcinoma. Antibody used at a dilution of 1:200.
The immunogen recognized by this antibody maps to a region between residue 1010 and the C-terminus (residue 1058) fo human Gem (nuclear organelle) Associated Protein 4 using the numbering given in entry NP_056536.1 (GeneID 50628).
Immunogen affinity purified
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Use in ICC/IF reported in scientific literature (PMID:10.1038/s41467-021-22627-w) Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
120 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publication using NB110-40591 in the following applications:
The product of this gene is part of a large complex localized to the cytoplasm, nucleoli, and to discrete nuclear bodies called Gemini bodies (gems). The complex functions in spliceosomal snRNP assembly in the cytoplasm, and regenerates spliceosomes required for pre-mRNA splicing in the nucleus. The encoded protein directly interacts with a DEAD box protein and several spliceosome core proteins. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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