Derlin 1 Antibody

Images

 
Western Blot: Derlin 1 Antibody [NB100-448] - Detection of Derlin protein in canine KRM microsomes.
Immunocytochemistry/ Immunofluorescence: Derlin 1 Antibody [NB100-448] - Derlin-1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Immunohistochemistry: Derlin 1 Antibody [NB100-448] - Staining of human liver.

Product Details

Summary
Reactivity Hu, Mu, Ca, ChHa, Rt, Bv, Ch, Xp, ZeSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Derlin 1 Antibody Summary

Immunogen
A synthetic peptide made to the C-terminal region of human Derlin 1 (between residues 200-251). [UniProt# Q9BUN8]
Localization
Endoplasmic reticulum
Predicted Species
Rat (100%), Xenopus (100%), Zebrafish (100%), Chicken (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DERL1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • Immunocytochemistry/Immunofluorescence 1:2000
  • Immunohistochemistry 5-10 ug/ml
  • Immunohistochemistry-Paraffin 5-10 ug/ml
Application Notes
This Derlin 1 antibody can be used for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot, where a band is seen at ~29 kDa. In ICC/IF, ER staining was observed in HeLa cells.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Derlin 1 Lysate (NBL1-09837)
Control Peptide
Derlin 1 Peptide (NB100-448PEP)
Publications
Read Publications using
NB100-448 in the following applications:

Reactivity Notes

Human, Canine and Chinese Hamster. Predicted to react with Xenopus, bovine, chicken, rat, zebrafish, and mouse based on 100% sequence homology. Mouse reactivity reported in scientific literature (PMID: 25377857)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Derlin 1 Antibody

  • DER-1
  • DER1Degradation in endoplasmic reticulum protein 1
  • Der1-like domain family, member 1
  • Der1-like protein 1
  • derlin-1
  • DERtrin-1
  • FLJ13784
  • FLJ42092
  • MGC3067
  • PRO2577

Background

Derlin 1 (degradation in endoplasmic reticulum protein 1) belongs to Derlin family and is a functional sub-unit of the ER-associated degradation (ERAD), a ubiquitin/proteasome dependent degradation process for proteins which fails to fold correctly or assemble into oligomeric complexes in ER. Derlin 1 forms a channel which permits the retrotranslocation of misfolded proteins into cytosol from ER wherein they undergo ubiquitination mediated proteasomal degradation. Derlin 1 can generate homo-/hetero-oligomers with DERL2 and DERL3, and interacts with several other proteins such as AMFR, VIMP/SELS, SEL1L, SYVN1 and VCP, as well as with SEL1L-SYVN1 and VCP-VIMP protein complexes. It also interacts with cytomegalovirus US11 protein, NGLY1 and YOD1, RNF103 etc. Expressed ubiquitously, it can get up-regulated in response to ER stress via ERN1-XBP1 pathway of the UPR/unfolded protein response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Derlin 1 Antibody (NB100-448)(2)

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Product General Protocols

View specific protocols for Derlin 1 Antibody (NB100-448): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Derlin 1 Antibody (NB100-448). (Showing 1 - 1 of 1 FAQs).

  1. We are interested in the product, NB100-448. Do you have any information about whether or not NB100-448 cross reacts with Drosophila after validation or based on sequence homology?
    • Our Derlin 1 antibody with catalogue number NB100-448 was raised to a synthetic peptide derived from the C-terminal region of human Derlin 1 (between residues 200-251). I have aligned the human and Drosophila sequences for you, and the sequence homology in this region is just 42%; as such NB100-448 is unlikely to recognise the Drosophila protein, although we do not have any testing data at present to confirm this. Human Drosophila. I am sorry for the inconvenience.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-448AF405
Alexa Fluor 488 Labeled NB100-448AF488
Alexa Fluor 647 Labeled NB100-448AF647
Alexa Fluor 700 Labeled NB100-448AF700
Biotin Labeled NB100-448B
DyLight 405 Labeled NB100-448V
DyLight 488 Labeled NB100-448G
DyLight 550 Labeled NB100-448R
DyLight 650 Labeled NB100-448C
FITC Labeled NB100-448F
HRP Labeled NB100-448H

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Bioinformatics

Gene Symbol DERL1