Western Blot: Derlin 1 Antibody [NB100-448] - Detection of Derlin protein in canine KRM microsomes.
Immunocytochemistry/ Immunofluorescence: Derlin 1 Antibody [NB100-448] - Derlin-1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Immunohistochemistry: Derlin 1 Antibody [NB100-448] - Staining of human liver.
This Derlin 1 antibody can be used for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot, where a band is seen at ~29 kDa. In ICC/IF, ER staining was observed in HeLa cells.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Human, Canine and Chinese Hamster. Predicted to react with Xenopus, bovine, chicken, rat, zebrafish, and mouse based on 100% sequence homology. Mouse reactivity reported in scientific literature (PMID: 25377857)
Alternate Names for Derlin 1 Antibody
DER1Degradation in endoplasmic reticulum protein 1
Der1-like domain family, member 1
Der1-like protein 1
Derlin 1 (degradation in endoplasmic reticulum protein 1) belongs to Derlin family and is a functional sub-unit of the ER-associated degradation (ERAD), a ubiquitin/proteasome dependent degradation process for proteins which fails to fold correctly or assemble into oligomeric complexes in ER. Derlin 1 forms a channel which permits the retrotranslocation of misfolded proteins into cytosol from ER wherein they undergo ubiquitination mediated proteasomal degradation. Derlin 1 can generate homo-/hetero-oligomers with DERL2 and DERL3, and interacts with several other proteins such as AMFR, VIMP/SELS, SEL1L, SYVN1 and VCP, as well as with SEL1L-SYVN1 and VCP-VIMP protein complexes. It also interacts with cytomegalovirus US11 protein, NGLY1 and YOD1, RNF103 etc. Expressed ubiquitously, it can get up-regulated in response to ER stress via ERN1-XBP1 pathway of the UPR/unfolded protein response.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Derlin 1 Antibody (NB100-448). (Showing 1 - 1 of 1 FAQs).
We are interested in the product, NB100-448. Do you have any information about whether or not NB100-448 cross reacts with Drosophila after validation or based on sequence homology?
Our Derlin 1 antibody with catalogue number NB100-448 was raised to a synthetic peptide derived from the C-terminal region of human Derlin 1 (between residues 200-251). I have aligned the human and Drosophila sequences for you, and the sequence homology in this region is just 42%; as such NB100-448 is unlikely to recognise the Drosophila protein, although we do not have any testing data at present to confirm this. HumanDrosophila. I am sorry for the inconvenience.