HRD1 Overexpression Lysate (Native)


Western Blot: HRD1 Overexpression Lysate (Adult Normal) [NBL1-16659] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HRD1.

Product Details

Applications WB
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Order Details

HRD1 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC220540 and based on accession number NM_172230. The protein contains a C-terminal DDK tag.
Homo sapiens synovial apoptosis inhibitor 1, synoviolin (SYVN1), transcript variant 2, mRNA.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer


  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
67.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Lysate Details for HRD1

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for HRD1 Overexpression Lysate (Native)

  • E3 ubiquitin-protein ligase synoviolin
  • EC 6.3.2
  • HRD1EC 6.3.2.-
  • KIAA1810Synovial apoptosis inhibitor 1
  • MGC40372
  • synovial apoptosis inhibitor 1, synoviolin


This gene encodes a protein involved in endoplasmic reticulum (ER)-associated degradation. The encoded protein removes unfolded proteins, accumulated during ER stress, by retrograde transport to the cytosol from the ER. This protein also uses the ubiquitin-proteasome system for additional degradation of unfolded proteins. This gene and the mitochondrial ribosomal protein L49 gene use in their respective 3' UTRs some of the same genomic sequence. Sequence analysis identified two transcript variants that encode different isoforms. [provided by RefSeq]. Transcript Variant: This variant (2) uses a different splice site than variant 1 and encodes the longer isoform (b). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: ICFlow
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Ye
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, Func, IHC, IHC-P, In vitro
Species: Hu, Mu, Ca, ChHa
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, PEP-ELISA
Species: Hu, Mu, Rt, Ca, Mk, Pm
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IP
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, Flow
Species: Hu, Mu, Rt, Ch, Sh
Applications: WB, Simple Western, Flow, ICC/IF
Species: Hu
Applications: WB, Flow, IHC
Species: Hu, Mu, Rt, Fi, Ha, Rb
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Hu
Applications: WB, IHC, IF
Species: Hu
Applications: WB, IP
Applications: WB

Publications for HRD1 Lysate (NBL1-16659) (0)

There are no publications for HRD1 Lysate (NBL1-16659).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for HRD1 Lysate (NBL1-16659) (0)

There are no reviews for HRD1 Lysate (NBL1-16659). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for HRD1 Lysate (NBL1-16659) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional HRD1 Overexpression Lysate (Native) Products

Related Products by Gene

Bioinformatics Tool for HRD1 Lysate (NBL1-16659)

Discover related pathways, diseases and genes to HRD1 Lysate (NBL1-16659). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for HRD1 Lysate (NBL1-16659)

Discover more about diseases related to HRD1 Lysate (NBL1-16659).

Pathways for HRD1 Lysate (NBL1-16659)

View related products by pathway.

PTMs for HRD1 Lysate (NBL1-16659)

Learn more about PTMs related to HRD1 Lysate (NBL1-16659).

Research Areas for HRD1 Lysate (NBL1-16659)

Find related products by research area.

Blogs on HRD1.

OS9: Taking proteins to the ER finish line
The OS9 protein is a lectin/glycoprotein that maintains endoplasmic reticulum (ER) quality control and ER-associated degradation (the so-called ERAD pathway) of newly synthesized proteins. It is essential for the recognition of terminally misfolded no...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol SYVN1

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBL1-16659 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought

HRD1 Recombinant Protein
ATF6 Antibody (70B1413.1)
ATF6 Antibody (70B1413.1)
ATF6 Antibody (70B1413.1)
HRD1 Antibody
HRD1 Antibody