HR6A/UBE2A Antibody

Images

 
Western Blot: HR6A/UBE2A Antibody [NB100-554] - Detection of Human Rad6 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit ...read more
Immunohistochemistry: HR6A/UBE2A Antibody [NB100-554] - Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti- Rad6 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: HR6A/UBE2A Antibody [NB100-554] - Whole cell lysate (50 ug) from 293T cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

     

Order Details

HR6A/UBE2A Antibody Summary

Immunogen
A synthetic peptide which maps to a region between residues 10 and 50 of human ubiquitin-conjugating enzyme E2A using the numbering given in Swiss-Prot entry P49459 (GeneID 7319).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UBE2A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:25000
  • Immunohistochemistry-Paraffin 1:500-1:2000
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Positive Control
HR6A/UBE2A Lysate (NBL1-17511)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HR6A/UBE2A Antibody

  • EC 6.3.2.19
  • HHR6A
  • HR6A
  • RAD6A
  • RAD6ARAD6 homolog A
  • UBC2
  • UBE2A
  • Ubiquitin carrier protein A
  • ubiquitin-conjugating enzyme E2 A
  • ubiquitin-conjugating enzyme E2A (RAD6 homolog)
  • Ubiquitin-protein ligase A

Background

The rad6 protein catalyzes the monoubiquitination of H2B. Rad6-directed H2B ubiquitination defines regions of active chromatin. The RAD6 pathway is also central to post-replicative DNA repair in eukaryotic cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol UBE2A
Entrez
Uniprot