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Dihydrolipoamide Dehydrogenase/DLD Antibody (OTI6D5)

Images

 
Western Blot: Lipoamide Dehydrogenase Antibody (6D5) [NBP2-01109] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Lipoamide Dehydrogenase (Right lane) cDNA for 48 hrs and lysed. ...read more
Immunocytochemistry/ Immunofluorescence: Lipoamide Dehydrogenase Antibody (6D5) [NBP2-01109] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Lipoamide Dehydrogenase.
Immunohistochemistry-Paraffin: Lipoamide Dehydrogenase Antibody (6D5) [NBP2-01109] - Staining of paraffin-embedded Human pancreas tissue using anti-Lipoamide Dehydrogenase mouse monoclonal antibody.
Flow Cytometry: Lipoamide Dehydrogenase Antibody (6D5) [NBP2-01109] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Lipoamide ...read more
Immunohistochemistry-Paraffin: Lipoamide Dehydrogenase Antibody (6D5) [NBP2-01109] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Lipoamide Dehydrogenase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Lipoamide Dehydrogenase Antibody (6D5) [NBP2-01109] - Staining of paraffin-embedded Human endometrium tissue using anti-Lipoamide Dehydrogenase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Lipoamide Dehydrogenase Antibody (6D5) [NBP2-01109] - Staining of paraffin-embedded Human lymph node tissue using anti-Lipoamide Dehydrogenase mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
OTI6D5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.82 mg/ml

Order Details

Dihydrolipoamide Dehydrogenase/DLD Antibody (OTI6D5) Summary

Immunogen
Full length human recombinant protein of human DLD(NP_000099) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
DLD
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
Theoretical MW
50.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-01109.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.82 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Dihydrolipoamide Dehydrogenase/DLD Antibody (OTI6D5)

  • 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex)
  • branched chain keto acid dehydrogenase complex
  • Diaphorase
  • dihydrolipoamide dehydrogenase (E3 component of pyruvate dehydrogenase complex
  • Dihydrolipoamide Dehydrogenase
  • dihydrolipoamide dehydrogenasemitochondrial
  • DLD
  • DLDD
  • DLDH
  • E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex
  • E3
  • EC 1.8.1
  • EC 1.8.1.4
  • GCSL
  • Glycine cleavage system L protein
  • glycine cleavage system protein L
  • LAD
  • Lipoamide Dehydrogenase
  • lipoamide reductase
  • lipoyl dehydrogenase
  • PHE3

Background

This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-01109
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P

Publications for Dihydrolipoamide Dehydrogenase/DLD Antibody (NBP2-01109)(1)


Showing Publication 1 - 1 of 1.
Publication using NBP2-01109 Applications Species
Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol DLD
Entrez