RAD18 Antibody (3H7)

Images

 
Western Blot: RAD18 Antibody (3H7) [H00056852-M01] - Analysis of RAD18 expression in transfected 293T cell line by RAD18 monoclonal antibody (M01), clone 3H7.Lane 1: RAD18 transfected lysate(56.2 KDa).Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: RAD18 Antibody (3H7) [H00056852-M01] - Analysis of monoclonal antibody to RAD18 on HeLa cell. Antibody concentration 25 ug/ml.
Immunohistochemistry-Paraffin: RAD18 Antibody (3H7) [H00056852-M01] - Analysis of monoclonal antibody to RAD18 on formalin-fixed paraffin-embedded human testis. Antibody concentration 1.5 ug/ml.
Western Blot: RAD18 Antibody (3H7) [H00056852-M01] - Analysis of RAD18 over-expressed 293 cell line, cotransfected with RAD18 Validated Chimera RNAi ( Cat # H00056852-R01V ) (Lane 2) or non-transfected control (Lane 1). ...read more
Western Blot: RAD18 Antibody (3H7) [H00056852-M01] - RAD18 monoclonal antibody (M01), clone 3H7. Analysis of RAD18 expression in PC-12.
Western Blot: RAD18 Antibody (3H7) [H00056852-M01] - RAD18 monoclonal antibody (M01), clone 3H7 Analysis of RAD18 expression in Hela S3 NE.
ELISA: RAD18 Antibody (3H7) [H00056852-M01] - Detection limit for recombinant GST tagged RAD18 is approximately 0.1ng/ml as a capture antibody.
Immunofluorescence: RAD18 Antibody (3H7) [H00056852-M01] - The antibody was used to detect RAD18 in U2os Cells. Anti-mouse 2nd antibody was used for detection (Red). DAPI was used for nuclear staining (Blue). This image ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, ICC/IF
Clone
3H7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

RAD18 Antibody (3H7) Summary

Immunogen
RAD18 (NP_064550, 332 a.a. ~ 430 a.a) partial recombinant protein with GST tag.EKEIDEIHSKYRKKHKSEFQLLVDQARKGYKKIAGMSQKTVTITKEDESTEKLSSVCMGQEDNMTSVTNHFSQSKLDSPEELEPDREEDSSSCIDIQEV
Specificity
RAD18 - RAD18 homolog (S. cerevisiae)
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
RAD18
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 25 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1.5 ug/ml
  • Immunofluorescence
Application Notes
Antibody reactive against recominant protein and cell lysate for Western Blot. Has also been used for immunofluorescence, immunohistochemistry (paraffin), RNAi validation and ELISA.
Reviewed Applications
Read 2 Reviews rated 3.5
using
H00056852-M01 in the following applications:

Publications
Read Publications using
H00056852-M01 in the following applications:

  • 3 publications
  • WB
    2 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26549024). Other species have not been tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RAD18 Antibody (3H7)

  • EC 6.3.2.-
  • hHR18
  • hRAD18
  • postreplication repair protein hRAD18p
  • Postreplication repair protein RAD18
  • RAD18 homolog (S. cerevisiae)
  • RAD18, S. cerevisiae, homolog
  • RING finger protein 73
  • RNF73E3 ubiquitin-protein ligase RAD18

Background

The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ELISA, ICC/IF, IHC, IHC-P, ICC/IF

Publications for RAD18 Antibody (H00056852-M01)(12)

We have publications tested in 2 confirmed species: Human, Human;Mouse.

We have publications tested in 2 applications: ICC/IF, WB.


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ICC/IF
(3)
WB
(2)
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Human
(3)
Human;Mouse
(1)
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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using H00056852-M01 Applications Species
Kanu N, Zhang T, Burrell RA et al. RAD18, WRNIP1 and ATMIN promote ATM signalling in response to replication stress. Oncogene. 2015 Nov 09 [PMID: 26549024] (ICC/IF, Human;Mouse) ICC/IF Human;Mouse
Kile AC, Chavez DA, Bacal J et al. HLTF's Ancient HIRAN Domain Binds 3' DNA Ends to Drive Replication Fork Reversal Mol. Cell. 2015 Jun 18 [PMID: 26051180] (WB, Human) WB Human
Sy SM, Jiang J, O WS et al. The ubiquitin specific protease USP34 promotes ubiquitin signaling at DNA double-strand breaks. Nucleic Acids Res. 2013 Jul 17 [PMID: 23863847]
Ghosal G, Leung JW, Nair BC et al. PCNA-binding protein C1orf124 is a regulator of translesion synthesis J Biol Chem 2012 Aug 17 [PMID: 22902628] (WB, ICC/IF, Human) WB, ICC/IF Human
Huehls AM, Wagner JM, Huntoon CJ, Karnitz LM. Identification of DNA Repair Pathways That Affect the Survival of Ovarian Cancer Cells Treated with a Poly(ADP-Ribose) Polymerase Inhibitor in a Novel Drug Combination Mol Pharmacol 2012 Oct [PMID: 22833573]
Panier S, Ichijima Y, Fradet-Turcotte A et al. Tandem Protein Interaction Modules Organize the Ubiquitin-Dependent Response to DNA Double-Strand Breaks Mol Cell 2012 Jun 26 [PMID: 22742833] (ICC/IF, Human) ICC/IF Human
Kato D, Waki M, Umezawa M et al. Phosphorylation of human INO80 is involved in DNA damage tolerance Biochem Biophys Res Commun. 2011 Dec 07 [PMID: 22166198]
Wong RP, Aguissa-Toure AH, Wani AA et al. Elevated expression of Rad18 regulates melanoma cell proliferation. Pigment Cell Melanoma Res. 2011 Dec 06 [PMID: 22145991]
Yanagihara H, Kobayashi J, Tateishi S et al. NBS1 Recruits RAD18 via a RAD6-like Domain and Regulates Pol ettab-Dependent Translesion DNA Synthesis. Mol Cell. 2011 Sep 02 [PMID: 21884979]
Wong RP, Lin H, Khosravi S et al. Tumour suppressor ING1b maintains genomic stability upon replication stress. Nucleic Acids Res. 2011 Jan 11 [PMID: 21227930]
Show All 12 Publications.

Reviews for RAD18 Antibody (H00056852-M01) (2) 3.52

Average Rating: 3.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using H00056852-M01:
Filter by Applications
WB
(1)
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Filter by Species
Human
(2)
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Images Ratings Applications Species Date Details
 RAD18 H00056852-M01
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reviewed by:
Lisa Moreau
Human 09/12/2017
View

Summary

Sample TestedHuman cells
SpeciesHuman
LotG4081-3H7
 
reviewed by:
Anonymous
WB Human 07/06/2009
View

Summary

ApplicationWestern Blot
Sample TestedHuman cell culture, Sample Amount: 50ug
SpeciesHuman
Lot0075036

Blocking

Blocking DetailsBlocking Buffer: 5%Milk in TBS, Blocking Time: 1 hour, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioDilution Ratio: 1/5000, Incubation Dilution Buffer: blocking buffer, Incubation Time: overnight, Incubation Temp: 2-8 C

Secondary Antibody

Secondary DescriptionSecondary Ab: Invitrogen, Secondary Ab Dilution Ratio: 1/10000

Details

Detection NotesMethod:Millipore ECL, ExposureTime:.5-1min, Ctrl:Wildtype(pos)&Knockout(neg), Bands:75kDa(specific), 60,100,110kDa(non-specific)

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RAD18 Antibody (H00056852-M01) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

3.5
5
0
4
1
3
1
2
0
1
0

Lisa Moreau
09/12/2017
Application:
Species: Human

 
Anonymous
07/06/2009
Application: WB
Species: Human

Bioinformatics

Gene Symbol RAD18
Entrez
OMIM
Uniprot