UBE2K/E2-25K Antibody (701316)

Images

 
Western blot shows lysates of human brain (cortex) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human UBE2K/E2‑25K Monoclonal Antibody (Catalog # MAB6609) followed by HRP-conjugated Anti-Mouse IgG ...read more
UBE2K/E2‑25K was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Mouse Anti-Human UBE2K/E2‑25K Monoclonal Antibody (Catalog # MAB6609) at 15 µg/mL overnight at 4 °C. Before ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC
Clone
701316
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

UBE2K/E2-25K Antibody (701316) Summary

Immunogen
E. coli-derived recombinant human UBE2K/E2‑25K
Ala2-Asn200
Accession # P61086
Specificity
Detects human UBE2K/E2‑25K in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
UBE2K
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL
Publications
Read Publication using
MAB6609 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for UBE2K/E2-25K Antibody (701316)

  • E2(25K)
  • E225K
  • E2-25K
  • EC 6.3.2.19
  • Hip2
  • HIP-2
  • huntingtin interacting protein 2
  • Huntingtin-interacting protein 2
  • HYPG
  • LIG
  • UbcH1
  • UBE2K
  • UBE2K-25K
  • ubiquitin carrier protein
  • ubiquitin-conjugating enzyme E2 K
  • Ubiquitin-conjugating enzyme E2(25K)
  • Ubiquitin-conjugating enzyme E2-25 kDa
  • Ubiquitin-conjugating enzyme E2-25K
  • ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast)
  • ubiquitin-protein ligase

Background

UBE2K (Ubiquitin-conjugating enzyme E2; also E2-25K, HIP-2, UBE2K-25K and LIG) is a 24-26 kDa cytosolic member of the UBE family of proteins. It is widely expressed (in neurons, monocytes and neutrophils), and acts to ubiquitinate proteins targeted for degradation. UBE2K serves as an E2 ubiquitin conjugating enzyme with a preference for ubiquitin chain generation through Lys48. It appears to polyubiquitinate targets once they have been monoubiquitinated, and to act as either a monomer or dimer. Human UBE2K is 200 amino acids (aa) in length. It contains one UBA domain (aa 160-200). There are two potential splice variants that show a deletion of aa 22-72 and 134-176, respectively. Human and mouse UBE2K are identical in aa sequence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-89522
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
H00007337-M01
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, KD, WB
NBP1-89150
Species: Hu, Rt
Applications: IHC, IHC-P, WB
NBP1-31302
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
H00011026-M01
Species: Hu
Applications: ELISA, WB
AF9024
Species: Mu, Rt
Applications: IHC, WB
NBP2-13961
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-54301
Species: Hu, Mu, Rt
Applications: CyTOF-ready, ELISA, IHC, IHC-P, WB
E2-622
Species: Hu
Applications: EnzAct
H00023327-P01
Species: Hu
Applications: ELISA, AP, PA, WB
NB100-91662
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP1-86509
Species: Hu
Applications: IHC, IHC-P
NBP2-27197
Species: Eq, Hu, Mu, Pm, Rt
Applications: WB
NBP3-13106
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NB200-103
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
U-100H
Species: Hu
Applications: EnzAct

Publications for UBE2K/E2-25K Antibody (MAB6609)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


Filter By Application
Western Blot
(1)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for UBE2K/E2-25K Antibody (MAB6609) (0)

There are no reviews for UBE2K/E2-25K Antibody (MAB6609). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for UBE2K/E2-25K Antibody (MAB6609) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our UBE2K/E2-25K Antibody (701316) and receive a gift card or discount.

Bioinformatics

Gene Symbol UBE2K
Uniprot