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Histone H2B Antibody

Images

 
Western Blot: Histone H2B Antibody [NBP2-42854] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2B antibody diluted at 1:1000. The HRP-conjugated ...read more
Immunocytochemistry/ Immunofluorescence: Histone H2B Antibody [NBP2-42854] - Analysis of Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: Histone H2B protein stained by Histone H2B antibody ...read more
Immunohistochemistry-Paraffin: Histone H2B Antibody [NBP2-42854] - Analysis of mouse middle brain. Histone H2B antibody diluted at 1:500.
Western Blot: Histone H2B Antibody [NBP2-42854] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2B antibody diluted at 1:5000. The HRP-conjugated ...read more
Immunohistochemistry-Paraffin: Histone H2B Antibody [NBP2-42854] - Analysis of H1299 xenograft, using Histone H2B antibody at 1:500 dilution.
Western Blot: Histone H2B Antibody [NBP2-42854] -The equivalent of 4 larvae was loadedA: 120hpf larvae4-12% NuPAGE Bis Tris precast gel diluted at 1:1000

Product Details

Summary
Reactivity Hu, Mu, Ze, Rt, Po, BvSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Histone H2B Antibody Summary

Immunogen
Full length human Histone H2B Recombinant protein.
Predicted Species
Rat (98%), Porcine (99%), Bovine (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HIST2H2BF
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:10000
Publications
Read Publication using
NBP2-42854 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for Histone H2B Antibody

  • FLJ35099
  • FLJ56780
  • FLJ56787
  • HIST1H2BK
  • histone 2, H2bf
  • histone cluster 2, H2bf
  • histone H2B type 2-F
  • Histone H2B
  • MGC131639

Background

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility tothe cellular machineries which require DNA as a template. Histones thereby play a central role in transcriptionregulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set ofpost-translational modifications of histones, also called histone code, and nucleosome remodeling

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Histone H2B Antibody (NBP2-42854)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Histone H2B Antibody (NBP2-42854) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HIST2H2BF