Histone H2B Antibody

Immunohistochemistry-Paraffin: Histone H2B Antibody [NBP2-42854] - Analysis of mouse middle brain. Histone H2B antibody diluted at 1:500.

Immunohistochemistry-Paraffin: Histone H2B Antibody [NBP2-42854] - Analysis of H1299 xenograft, using Histone H2B antibody at 1:500 dilution.

Western Blot: Histone H2B Antibody [NBP2-42854] -The equivalent of 4 larvae was loadedA: 120hpf larvae4-12% NuPAGE Bis Tris precast gel diluted at 1:1000

Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2B antibody (NBP2-42854) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Histone H2B antibody detects Histone H2B protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min.Green: Histone H2B protein stained by Histone H2B antibody (NBP2-42854) diluted at 1:500.Blue: Hoechst 33342 staining.

Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2B antibody (NBP2-42854) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Ze, Rt, Po, Bv
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Histone H2B Antibody Summary

• Description: Novus Biologicals Rabbit Histone H2B Antibody (NBP2-42854) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Histone H2B Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human Histone H2B Recombinant protein.
• Predicted Species: Rat (98%), Porcine (99%), Bovine (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: H2BC18
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:10000

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Histone H2B Antibody

• FLJ35099
• FLJ56780
• FLJ56787
• HIST1H2BK
• histone 2, H2bf
• histone cluster 2, H2bf
• histone H2B type 2-F
• Histone H2B
• MGC131639

Background

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility tothe cellular machineries which require DNA as a template. Histones thereby play a central role in transcriptionregulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set ofpost-translational modifications of histones, also called histone code, and nucleosome remodeling

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Histone H2B Antibody (NBP2-42854)(1)

Publication using NBP2-42854

• Lyu Y, Yang Y, Talwar V et al Hypoxia-inducible factor 1 recruits FACT and RNF20/40 to mediate histone ubiquitination and transcriptional activation of target genes Cell Rep 2024-03-22 [PMID: 38517892] (WB, Human)

Bioinformatics

• Gene Symbol: H2BC18