Recombinant Human UbcH5b/UBE2D2 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

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Recombinant Human UbcH5b/UBE2D2 Protein, CF Summary

Details of Functionality
Recombinant Human UbcH5b/UBE2D2 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UbcH5b/UBE2D2 concentration of 0.1-1 μM.
Source
E. coli-derived human UbcH5b/UBE2D2 protein
Accession #
Protein/Peptide Type
Recombinant Enzymes
Gene
UBE2D2
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Applications/Dilutions

Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
E2-622 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Buffer

X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human UbcH5b/UBE2D2 Protein, CF

  • E2(17)KB2
  • EC 6.3.2.19
  • PUBC1
  • UBC4/5
  • UBC4ubiquitin-conjugating enzyme E2D 2 (homologous to yeast UBC4/5)
  • UBC5B
  • UBCH4
  • UbcH5b
  • UBE2D2
  • Ubiquitin carrier protein D2
  • ubiquitin-conjugating enzyme E2 D2
  • Ubiquitin-conjugating enzyme E2(17)KB 2
  • Ubiquitin-conjugating enzyme E2-17 kDa 2
  • ubiquitin-conjugating enzyme E2D 2 (UBC4/5 homolog, yeast)
  • Ubiquitin-protein ligase D2

Background

Ubiquitin-conjugating Enzyme H5b (UbcH5b), also known as Ubiquitin-conjugating Enzyme E2D 2 (UBE2D2), is a widely expressed member of the Ubiquitin-conjugating (E2) enzyme family (1). The protein has a predicted molecular weight of 16.5 kDa. UbcH5b/UBE2D2 localizes to both the nucleus and the cytoplasm (2). The human protein shares 100% and 92% amino acid sequence identity with the mouse and rat orthologs, respectively. This enzyme has an E2 catalytic core domain that contains an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin (3). UbcH5b/UBE2D2 is capable of mediating the formation of Ubiquitin chains linked through Lys11, Lys48, or Lys63 (4). Working with the SCF(Fbxw2) and MDM2/HDM2 Ubiquitin ligases (E3s), UbcH5b/UBE2D2 mediates the ubiquitination and degradation of the transcription factors GCM1 and p53, respectively (5-7). Along with UBE2N/Ubc13, UbcH5b/UBE2D2 may have a role in the endocytosis and endolysosomal degradation of MHC class I molecules (8). Non-proteolytic ubiquitination of TRIM5-alpha by UbcH5b/UBE2D2 has been reported to block HIV reverse transcription (9). Pathologically, UBE2D family members may be critical targets of cadmium during cadmium-induced renal toxicity (10). Additionally, overexpression of UbcH5b/UBE2D2 has been linked to inflammatory bowel disease (11).

  1. Jensen, J.P. et al. (1995) J. Biol. Chem. 270:30408.
  2. Umebayashi, K. et al. (2008) Mol. Biol. Cell 19:3454.
  3. Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.
  4. Ordureau, A. et al. (2008) Biochem. J. 409:43.
  5. Chiang, M.H. et al. (2008) Biol. Reprod. 79:914.
  6. Saville, M.K. et al. (2004) J. Biol. Chem. 279:42169.
  7. Iyappan, S. et al. (2010) J. Biol. Chem. 285:33065.
  8. Duncan, L.M. et al. (2006) EMBO J. 25:1635.
  9. Yamauchi, K. et al. (2008) FEBS J. 275:1540.
  10. Tokumoto, M. et al. (2011) J. Toxicol. Sci. 36:191.
  11. Hetzenecker, A.M. et al. (2012) Digestion 86:34.

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Publications for UbcH5b/UBE2D2 (E2-622)(21)

We have publications tested in 2 confirmed species: Human, N/A.

We have publications tested in 4 applications: Bioassay, Enzyme Assay, Ubiquitination, in vitro ubiquitination.


Filter By Application
Bioassay
(16)
Enzyme Assay
(1)
Ubiquitination
(2)
in vitro ubiquitination
(1)
All Applications
Filter By Species
Human
(10)
N/A
(6)
All Species
Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using E2-622 Applications Species
M Bosshard, R Aprigliano, C Gattiker, V Palibrk, E Markkanen, P Hoff Backe, S Pellegrino, FL Raymond, G Froyen, M Altmeyer, M Bjørås, GL Dianov, B van Loon Impaired oxidative stress response characterizes HUWE1-promoted X-linked intellectual disability Sci Rep, 2017;7(1):15050. 2017 [PMID: 29118367] (Bioassay, Human) Bioassay Human
N Lian, X Liu, X Wang, Y Zhou, H Li, J Li, T Mao COP1 mediates dark-specific degradation of microtubule-associated protein WDL3 in regulating Arabidopsis hypocotyl elongation Proc. Natl. Acad. Sci. U.S.A., 2017;114(46):12321-12326. 2017 [PMID: 29087315] (Bioassay, Human) Bioassay Human
B Zhang, M Holmlund, S Lorrain, M Norberg, L Bakó, C Fankhauser, O Nilsson BLADE-ON-PETIOLE proteins act in an E3 ubiquitin ligase complex to regulate PHYTOCHROME INTERACTING FACTOR 4 abundance Elife, 2017;6(0):. 2017 [PMID: 28826468] (Bioassay, Human) Bioassay Human
ZQ Fan, JY Chen, JF Kuang, WJ Lu, W Shan The Banana Fruit SINA Ubiquitin Ligase MaSINA1 Regulates the Stability of MaICE1 to be Negatively Involved in Cold Stress Response Front Plant Sci, 2017;8(0):995. 2017 [PMID: 28659946] (Bioassay) Bioassay
R Tawo, W Pokrzywa, É Kevei, ME Akyuz, V Balaji, S Adrian, J Höhfeld, T Hoppe The Ubiquitin Ligase CHIP Integrates Proteostasis and Aging by Regulation of Insulin Receptor Turnover Cell, 2017;169(3):470-482.e13. 2017 [PMID: 28431247] (Bioassay) Bioassay
X Xu, PK Kathare, VN Pham, Q Bu, A Nguyen, E Huq Reciprocal proteasome-mediated degradation of PIFs and HFR1 underlying photomorphogenic development in Arabidopsis Development, 2017;0(0):. 2017 [PMID: 28420710] (Bioassay) Bioassay
X Wei, X Wang, J Zhan, Y Chen, W Fang, L Zhang, H Zhang Smurf1 inhibits integrin activation by controlling Kindlin-2 ubiquitination and degradation J. Cell Biol., 2017;0(0):. 2017 [PMID: 28408404] (Bioassay) Bioassay
J Zhu, KY Lee, KA Jewett, HY Man, HJ Chung, NP Tsai Epilepsy-associated gene Nedd4-2 mediates neuronal activity and seizure susceptibility through AMPA receptors PLoS Genet, 2017;13(2):e1006634. 2017 [PMID: 28212375] (Bioassay, Human) Bioassay Human
Francesca Nazio Fine-tuning of ULK1 mRNA and protein levels is required for autophagy oscillation J. Cell Biol, 2016;215(6):841-856. 2016 [PMID: 27932573] (Bioassay, Human) Bioassay Human
Identification and Characterization of MCM3 as a Novel KEAP1 Substrate J Biol Chem, 2016;0(0):. 2016 [PMID: 27621311] (Ubiquitination, Human) Ubiquitination Human
Show All 21 Publications.

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Bioinformatics

Gene Symbol UBE2D2
Entrez
Uniprot