Hormone-sensitive Lipase/HSL [p Ser563] Antibody - Azide and BSA Free

Western Blot: Hormone-sensitive Lipase/HSL [p Ser563] Antibody [NBP3-05457] - Western blot analysis of extracts of various cell lines, using Hormone-sensitive Lipase/HSL antibody (NBP3-05457) at 1:1000 dilution.3T3L1 cells were treated by lambda-PP mixed solution (1ul) at 30C for 30 minutes.H9C2 cells were treated with Isoproterenol (10 uM for 20 min) at 37C. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Western Blot: Hormone-sensitive Lipase/HSL [p Ser563] Antibody - Azide and BSA Free [Hormone-sensitive Lipase/HSL] - Western blot analysis of lysates from Mouse brown fat using Hormone-sensitive Lipase/HSL Rabbit pAb at 1:5000 dilution incubated overnight at 4C. Mouse brown fat were treated by lambda-PP mixed solution (1 ul) at 30C for 30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 30 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Hormone-sensitive Lipase/HSL [p Ser563] Antibody - Azide and BSA Free Summary

• Immunogen: A synthetic phosphorylated peptide around S563 of rat Hormone-sensitive Lipase/HSL (XP_006228456.1). RRSVS
• Modification: p Ser563
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LIPE
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:1000
• Application Notes: Hormone-sensitive Lipase/HSL [p Ser563] antibody validated for WB from a verified customer review.

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Hormone-sensitive Lipase/HSL [p Ser563] Antibody - Azide and BSA Free

• hormone-sensitive lipase testicular isoform
• Hormonesensitive Lipase
• Hormone-sensitive Lipase
• HSL
• HSLEC 3.1.1.79
• LHS
• lipase, hormone-sensitive
• LIPE

Background

Hormone-sensitive lipase (HSL) is an intracellular neutral lipase found in a variety of tissues, but primarily in adipose and steroidogenic tissues. It hydrolyzes triglycerides into free fatty acids and cholesteryl esters into free cholesterol for steroid hormone synthesis.

HSL is the major enzyme responsible for the mobilization of free fatty acids from adopise tissue, and HSL antibodies are useful tools for lipid and metabolism research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Review for Hormone-sensitive Lipase/HSL Antibody (NBP3-05457) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Robin Wilson WB Mouse 05/05/2021

Summary

• Application: Western Blot
• Sample Tested: Differentiated adipocytes
• Species: Mouse
• Lot: 2158340101

Comments

• Comments: ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***

Bioinformatics

• Gene Symbol: LIPE