Mouse HO-1/HMOX1/HSP32 ELISA Kit (Colorimetric)

ELISA: Mouse HO-1/HMOX1/HSP32 ELISA Kit (Colorimetric) [NBP3-18029] - Samples were spiked with high concentrations of Mouse HO-1/HMOX1/HSP32 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse HO-1/HMOX1/HSP32 ELISA Kit (Colorimetric) [NBP3-18029] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 62.5-4000 pg/mL
• Sensitivity: 37.5 pg/mL

Mouse HO-1/HMOX1/HSP32 ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 62.5-4000 pg/mL
• Sensitivity: 37.5 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 6.00
• Intra-Assay: %CV < 5.45
• Spike Recovery: 84-102%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: HMOX1

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse HO-1/HMOX1/HSP32 ELISA Kit (Colorimetric)

• bK286B10
• EC 1.14.99.3
• heat shock protein, 32-kD
• heme oxygenase (decycling) 1
• HMOX1
• HO
• HO1
• HO-1
• HO-1heme oxygenase 1
• HSP32

Background

Stressed mammalian cells up-regulate heme oxygenase 1 (HO-1), which catabolizes heme to biliverdin, carbon monoxide, and free iron. Results provide genetic evidence that up-regulation of HO-1 serves as an adaptive mechanism to protect cells from oxidative damage during stress (1). When cells are injured they release their contents, resulting in a local accumulation of free heme proteins and heme. HO-1 plays a crucial role in the inflammatory process during wound healing (2). TNFalpha accelerates inflammatory responses by down-regulating HO-1 expression in human monocytes. TNF antagonists may block this TNF-dependent suppression of HO-1 expression, resulting in an amelioration of inflammation (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: HMOX1