hnRNP F Antibody

Images

 
Western Blot: hnRNP F Antibody [NBP1-33607] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: hnRNP F Antibody [NBP1-33607] - Paraformaldehyde-fixed A431, using hnRNP F (NBP1-33607) antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: hnRNP F Antibody [NBP1-33607] - Paraffin-embedded mouse uterus. hnRNP F antibody [N1N3] diluted at 1:500.
Immunohistochemistry-Paraffin: hnRNP F Antibody [NBP1-33607] - Paraffin-embedded A549 Xenograft, using antibody at 1:100 dilution.
Immunoprecipitation: hnRNP F Antibody [NBP1-33607] - Immunoprecipitation of hnRNP F protein from 293T whole cell extracts using 5 ug of hnRNP F antibody [N1N3]. Western blot analysis was performed using hnRNP F antibody ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Mk, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.72 mg/ml

Order Details

hnRNP F Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human hnRNP F. The exact sequence is proprietary.
Localization
Nucleus, nucleoplasm
Predicted Species
Rat (98%), Primate (100%), Bovine (97%), Monkey (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HNRNPF
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Rhesus Monkey (100%) and Chimpanzee (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.72 mg/ml
Purity
Immunogen affinity purified

Alternate Names for hnRNP F Antibody

  • heterogeneous nuclear ribonucleoprotein F
  • hnRNP F
  • HNRPFHnRNP F protein
  • mcs94-1
  • MGC110997
  • Nucleolin-like protein mcs94-1
  • OK/SW-cl.23

Background

Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to pre-mRNA processing and transport, and also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II. hnRNP complexes are the major constituents of the spliceosome and in particular, the hnRNP A1 protein is one of the major premRNA/ mRNA binding proteins and also one of the most abundant proteins in the nucleus. hnRNP A1 and A2/B1 regulate the processing of pre-mRNA by directly antagonizing the association of various splicing factors and by influencing the splice site selection on pre-mRNA. The majority of hnRNP proteins components are localized to the nucleus; however some shuttle between the nucleus and the cytoplasm. Most hnRNP proteins, including hnRNP C1 and C2, contain one or more RNA binding domains and are implicated in the processing of pre-mRNA. hnRNPs F and H are largely related factors that preferentially associate with poly(rG) regions on RNA. Isoforms of these proteins are often generated by alternative processing of the premRNA and by posttranslational modifications such as phosphorylation on serines and threonines and methylation of arginines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol HNRNPF