MADD Antibody

Images

 
Western Blot: MADD Antibody [NBP1-77033] - Whole cell lysates from the indicated cell lines with MADD antibody at 1:250 dilution.
Immunocytochemistry/ Immunofluorescence: MADD Antibody [NBP1-77033] - Immunofluorescence of MADD in Hela cells with MADD antibody at 20 ug/mL.
Immunocytochemistry/ Immunofluorescence: MADD Antibody [NBP1-77033] - human spleen tissue with MADD antibody at 10 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

MADD Antibody Summary

Immunogen
Antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human MADD. The immunogen is located within the last 50 amino acids of MADD.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MADD
Purity
Ion exchange chromatography
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
MADD antibody can be used for detection of MADD by Western blot at 1:250 to 1:500 dilution. 200 to 220 kDa bands should be detected.
Theoretical MW
183 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
MADD Peptide (NBP1-77033PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Ion exchange chromatography

Alternate Names for MADD Antibody

  • DENNFLJ36300
  • Differentially expressed in normal and neoplastic cells
  • FLJ35600
  • IG20
  • Insulinoma glucagonoma clone 20
  • insulinoma-glucagonoma protein 20
  • KIAA0358RAB3GEP
  • MAP kinase-activating death domain protein
  • MAP-kinase activating death domain
  • Rab3 GDP/GTP exchange factor

Background

MAP kinase-activating death domain protein (MADD) was initially identified as the type 1 tumor necrosis factor receptor (TNFR1) associated protein though their death domains. Overexpression of MADD activates MAP kinases ERK and JNK and induces the phosphorylation of cytosolic phospholipase A2. MADD shares 98% identity with DENN (for differentially expressed in neoplastic vs. normal cells), which was recently identified as a substrate for c-jun N-terminal kinase 3 (JNK3). MADD has greater than 94% overall identity to a GDP/GTP exchange protein Rab3-GEP. MADD is 87% identical to KIAA0358, a brain protein of unknown function. Identification of MADD as a component of the TNFR1 signaling complex and the similarity between MADD and Rab3-GEP provides a connection between TNFR1 activation and downstream MAP kinase activity through a guanine-nucleotide exchange protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Calculators

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Bioinformatics

Gene Symbol MADD
Uniprot