MADD Antibody - BSA Free

Western Blot: MADD Antibody [NBP1-77033] - Whole cell lysates from the indicated cell lines with MADD antibody at 1:250 dilution.

Immunocytochemistry/Immunofluorescence: MADD Antibody [NBP1-77033] - human spleen tissue with MADD antibody at 10 ug/ml.

Immunocytochemistry/ Immunofluorescence: MADD Antibody - BSA Free [NBP1-77033] - Immunofluorescence of MADD in Hela cells with MADD antibody at 20 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

MADD Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit MADD Antibody - BSA Free (NBP1-77033) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human MADD. The immunogen is located within the last 50 amino acids of MADD. Amino Acid Squence: AAVHSSEEDLRTPPRPVSS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MADD
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 10-20 ug/ml
  • Western Blot 1-2mg/mL
• Application Notes: 200 to 220 kDa bands should be detected in Western Blot.
• Theoretical MW: 200-220 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  MADD Peptide (NBP1-77033PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Ion exchange chromatography

Alternate Names for MADD Antibody - BSA Free

• DENNFLJ36300
• Differentially expressed in normal and neoplastic cells
• FLJ35600
• IG20
• Insulinoma glucagonoma clone 20
• insulinoma-glucagonoma protein 20
• KIAA0358RAB3GEP
• MAP kinase-activating death domain protein
• MAP-kinase activating death domain
• Rab3 GDP/GTP exchange factor

Background

MAP kinase-activating death domain protein (MADD) was initially identified as the type 1 tumor necrosis factor receptor (TNFR1) associated protein though their death domains. Overexpression of MADD activates MAP kinases ERK and JNK and induces the phosphorylation of cytosolic phospholipase A2. MADD shares 98% identity with DENN (for differentially expressed in neoplastic vs. normal cells), which was recently identified as a substrate for c-jun N-terminal kinase 3 (JNK3). MADD has greater than 94% overall identity to a GDP/GTP exchange protein Rab3-GEP. MADD is 87% identical to KIAA0358, a brain protein of unknown function. Identification of MADD as a component of the TNFR1 signaling complex and the similarity between MADD and Rab3-GEP provides a connection between TNFR1 activation and downstream MAP kinase activity through a guanine-nucleotide exchange protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MADD
• Uniprot:
  • AAD12154(Human)