MLX Antibody

Images

 
Western blot shows nuclear extracts of PC‑3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MLX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4186) followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

MLX Antibody Summary

Immunogen
E. coli-derived recombinant human MLX
Glu197-Tyr298
Accession # EAW60842
Specificity
Detects human MLX in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MLX
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunocytochemistry 5-15 ug/mL
Publications
Read Publication using
AF4186 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MLX Antibody

  • BHLHD13
  • bHLHd13Max-like bHLHZip protein
  • BigMax alpha
  • BigMax protein
  • Class D basic helix-loop-helix protein 13
  • MAD7
  • MAX-like protein X
  • MLX
  • MXD7
  • TCFL4
  • TCFL4Protein BigMax
  • transcription factor-like 4
  • Transcription factor-like protein 4

Background

MLX (Max-like protein X; also known as transcription factor-like protein 4) is a 30 kDa member of the Max-like bHLHZip family of proteins. It is widely expressed and is found in both cytoplasm and nucleus. MLX is a transcriptional repressor when dimerized with MAD family members and a transcriptional activator when dimerized with MONDO family members. Human MLX is 298 amino acids (aa) in length. It contains one DNA-binding region (aa 76‑87), an HLH domain (aa 127‑192) and a Leu-zipper domain (aa 140‑160). There are three alternate splice short forms. One shows the deletion of aa 15‑68, a second shows deletion of aa 15‑68 and 81‑110, and a third shows a 3 aa substitution for aa 111‑298. Over aa 197‑298, human MLX is 99% identical to mouse and canine MLX.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MLX Antibody (AF4186)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ChIP.


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ChIP
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for MLX Antibody (AF4186) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for MLX Antibody (AF4186)

Discover related pathways, diseases and genes to MLX Antibody (AF4186). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Pathways for MLX Antibody (AF4186)

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PTMs for MLX Antibody (AF4186)

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Blogs on MLX.

ChREBP, a glucose sensitive transcription factor with role in glucose-lipids homeostasis and cancer
ChREBP (carbohydrate response element-binding protein) is a glucose responsive basic helix-loop-helix/leucine zipper (bHLH/LZ) transcription factor that binds MLX and then carbohydrate response element /ChoRE for the induction of genes involved in...  Read full blog post.

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Bioinformatics

Gene Symbol MLX
Uniprot