HNF-4 alpha/NR2A1 Antibodies

HAF018). Specific bands were detected for HNF-4a/NR2A1 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF018). Specific bands were detected for HNF-4a/NR2A1 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. Gels were loaded with 10 µg of cytoplasmic (Cyto) and 5 µg of nuclear (Nuc) extracts. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human HNF-4a/ NR2A1 Monoclonal Antibody (Catalog # MAB4605) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for HNF-4a/NR2A1 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

MAB0041, open histogram) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules." class="big_lightbox"> MAB0041, open histogram) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules." class="big_thumb" />

SC019B) was stained with Mouse Anti-Human HNF-4 alpha/NR2A1 Monoclonal Antibody (Catalog # MAB4605, filled histogram or isotype control antibody (Catalog # MAB0041, open histogram) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules." class="big_lightbox"> SC019B) was stained with Mouse Anti-Human HNF-4 alpha/NR2A1 Monoclonal Antibody (Catalog # MAB4605, filled histogram or isotype control antibody (Catalog # MAB0041, open histogram) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules." class="big_thumb" />

HAF019). A specific band for HNF-4a/NR2A1 was detected at approximately 55-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF019). A specific band for HNF-4a/NR2A1 was detected at approximately 55-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Goat Anti-Human HNF-4a/NR2A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4605) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band for HNF-4a/NR2A1 was detected at approximately 55-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox"> HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />