Histone Deacetylase 4/HDAC4 Antibody

Immunoprecipitation: Histone Deacetylase 4/HDAC4 Antibody [NB600-1262] - Rabbit Anti-HDAC4 was used to immunoprecipitate HDAC4 from HeLa whole cell extract. Detection antibody: Rabbit Anti-HDAC4 Lanes 1. 10 ug antibody 2. 5 ug antibody 3. Negative control: No cell extract 4. Positive control: HeLa whole cell extract

Product Discontinued

Histone Deacetylase 4/HDAC4 Antibody Summary

• Immunogen: Synthetic peptide: DQPVELLNPARVNHM with C-terminal added lysine, conjugated to KLH, corresponding to amino acids 14-28 of Human HDAC4.
• Localization: Cytoplasmic and Nuclear
• Specificity: HDAC4
• Predicted Species: Chicken (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HDAC4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:250
  • Immunoprecipitation 10-20 ug
  • Microarray
  • Western Blot 1:500-1:1000
• Theoretical MW: 140 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  NIH 3T3 Whole Cell Lysate
  HeLa Nuclear Cell Lysate
  Histone Deacetylase 4/HDAC4 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4) and 1.0% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Histone Deacetylase 4/HDAC4 Antibody

• HA6116
• HD4EC 3.5.1.98
• HDAC4
• HDAC-A
• HDACABDMR
• Histone Deacetylase 4
• histone deacetylase A
• KIAA0288AHO3

Background

Chromatin is a highly specialized structure composed of tightly compacted chromosomal DNA. Gene expression within the nucleus is controlled, in part, by a host of protein complexes which continuously pack and unpack the chromosomal DNA. One of the known mechanisms of this packing and unpacking process involves the acetylation and deacetylation of the histone proteins comprising the nucleosomal core. Acetylated histone proteins confer accessibility of the DNA template to the transcriptional machinery for expression. Histone deacetylases (HDACs) are chromatin remodeling factors that deacetylate histone proteins and thus, may act as transcriptional repressors. HDACs are classified by their sequence homology to the yeast HDACs and there are currently 2 classes. Class I proteins are related to Rpd3 and members of class II resemble Hda1p.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Histone Deacetylase 4/HDAC4 Antibody (NB600-1262)(1)

Publication using NB600-1262

• Geng, L, Cuneo, K C, Fu, A, Tu, T, Atadja, P W, Hallahan, D E. Histone Deacetylase (HDAC) Inhibitor LBH589 Increases Duration of {gamma}-H2AX Foci Confines HDAC4 to the Cytoplasm in Irradiated Non-Small Cell Lung Cancer, . 66, 11298-11304. 2006-01-01 [PMID: 17145876]

Bioinformatics

• Gene Symbol: HDAC4
• Entrez:
  • 9759(Human)
  • 208727(Mouse)
  • 363287(Rat)
• Uniprot:
  • P56524(Human)