HDAC7 Antibody

Images

 
Western Blot: HDAC7 Antibody [NBP1-44092] - Lane 1: mouse brain homogenate. Lane 2: mouse brain homogenate blocked with peptide. Load: 4 ug per lane. Primary antibody: HDAC7 antibody at 1ug/mL for overnight at 4C. ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.16 mg/ml

Order Details

HDAC7 Antibody Summary

Immunogen
A synthetic peptide from the N terminal region of HDAC7.
Specificity
This antibody is specific to HDAC7
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HDAC7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • ELISA 1:20000 - 1:60000
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
Reviewed Applications
Read 1 Review rated 5
using
NBP1-44092 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
1.16 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HDAC7 Antibody

  • DKFZP586J0917
  • EC 3.5.1.98
  • HD7
  • HD7A
  • HDAC7AFLJ99588
  • histone deacetylase 7
  • Histone deacetylase 7ADKFZp586J0917

Background

HDAC7 is a member of the class II mammalian histone deacetylases, which plays an important role in modulating the eukaryotic chromatin structure. Human HDAC7 is composed of 912 amino acid residues. Although HDAC7 is localized mostly to the cell nucleus, it is also found in the cytoplasm, suggesting nucleo-cytoplasmic shuttling. The histone deacetylase activity of HDAC7 maps to a carboxy-terminal domain and is dependent on interaction with class I HDACs in the nucleus. It is an active component of different transcriptional corepressor complexes that can be recruited to specific promoter regions via interactions with a growing number of sequence specific transcriptional factors. HDAC7 catalyzes removal of acetyl-groups from acetyl-lysines of histones and promotes compaction of chromatin in these regions, leading to the inhibition of gene transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HDAC7 Antibody (NBP1-44092) (0)

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Review for HDAC7 Antibody (NBP1-44092) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-44092:
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Images Ratings Applications Species Date Details
Western Blot HDAC7 NBP1-44092
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reviewed by:
Corey.
WB Human 09/26/2013
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Summary

ApplicationWestern Blot
Sample TestedHuman MCF-7 cell lysate
SpeciesHuman

Blocking

Blocking Details5% NFDM

Primary Anitbody

Dilution Ratio1:500, 4C O/N

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HDAC7 Antibody (NBP1-44092) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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1
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Corey.
09/26/2013
Application: WB
Species: Human

Bioinformatics

Gene Symbol HDAC7
Entrez
Uniprot