Western Blot: HDAC7 Antibody [NBP1-44092] - Lane 1: mouse brain homogenate. Lane 2: mouse brain homogenate blocked with peptide. Load: 4 ug per lane. Primary antibody: HDAC7 antibody at 1ug/mL for overnight at 4C. ...read more
This antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
HDAC7 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N terminal region of human HDAC7. (Uniprot: Q8WUI4)
Cell nucleus, Cytoplasm
Immunogen affinity purified
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This product has been tested by ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~103kDa and isoforms at ~70, and 40 kDa corresponding to the appropriate cell lysate or extract.
Read 1 Review rated 5 using NBP1-44092 in the following applications:
Cross-reactivity with HDAC7 from other sources has not been determined
Packaging, Storage & Formulations
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% Sodium Azide
Immunogen affinity purified
Alternate Names for HDAC7 Antibody
histone deacetylase 7
Histone deacetylase 7ADKFZp586J0917
HDAC7 is a member of the class II mammalian histone deacetylases, which plays an important role in modulating the eukaryotic chromatin structure. Human HDAC7 is composed of 912 amino acid residues. Although HDAC7 is localized mostly to the cell nucleus, it is also found in the cytoplasm, suggesting nucleo-cytoplasmic shuttling. The histone deacetylase activity of HDAC7 maps to a carboxy-terminal domain and is dependent on interaction with class I HDACs in the nucleus. It is an active component of different transcriptional corepressor complexes that can be recruited to specific promoter regions via interactions with a growing number of sequence specific transcriptional factors. HDAC7 catalyzes removal of acetyl-groups from acetyl-lysines of histones and promotes compaction of chromatin in these regions, leading to the inhibition of gene transcription.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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