HDAC7 Antibody

Western Blot: HDAC7 Antibody [NBP1-44092] - Lane 1: mouse brain homogenate. Lane 2: mouse brain homogenate blocked with peptide. Load: 4 ug per lane. Primary antibody: HDAC7 antibody at 1ug/mL for overnight at 4C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 103kDa/ 103, 70, and 40kDa for HDAC7. Other band(s): HDAC7 isoforms 70 and 40kDa.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

HDAC7 Antibody Summary

• Description: This antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: HDAC7 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N terminal region of human HDAC7. (Uniprot: Q8WUI4)
• Localization: Cell nucleus, Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HDAC7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:20000-1:60000
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Western Blot 1 ug/ml
• Application Notes: This product has been tested by ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~103kDa and isoforms at ~70, and 40 kDa corresponding to the appropriate cell lysate or extract.

Reactivity Notes

Cross-reactivity with HDAC7 from other sources has not been determined

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for HDAC7 Antibody

• DKFZP586J0917
• EC 3.5.1.98
• HD7
• HD7a
• HDAC7
• HDAC7A
• HDAC7AFLJ99588
• histone deacetylase 7
• Histone deacetylase 7ADKFZp586J0917

Background

HDAC7 is a member of the class II mammalian histone deacetylases, which plays an important role in modulating the eukaryotic chromatin structure. Human HDAC7 is composed of 912 amino acid residues. Although HDAC7 is localized mostly to the cell nucleus, it is also found in the cytoplasm, suggesting nucleo-cytoplasmic shuttling. The histone deacetylase activity of HDAC7 maps to a carboxy-terminal domain and is dependent on interaction with class I HDACs in the nucleus. It is an active component of different transcriptional corepressor complexes that can be recruited to specific promoter regions via interactions with a growing number of sequence specific transcriptional factors. HDAC7 catalyzes removal of acetyl-groups from acetyl-lysines of histones and promotes compaction of chromatin in these regions, leading to the inhibition of gene transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for HDAC7 Antibody (NBP1-44092) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Corey . WB Human 09/26/2013

Summary

• Application: Western Blot
• Sample Tested: Human MCF-7 cell lysate
• Species: Human

Bioinformatics

• Gene Symbol: HDAC7
• Entrez:
  • 51564(Human)
• Uniprot:
  • Q8WUI4(Human)