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HDAC7 Antibody

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Western Blot: HDAC7 Antibody [NBP1-44092] - Lane 1: mouse brain homogenate. Lane 2: mouse brain homogenate blocked with peptide. Load: 4 ug per lane. Primary antibody: HDAC7 antibody at 1ug/mL for overnight at 4C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 103kDa/ 103, 70, and 40kDa for HDAC7. Other band(s): HDAC7 isoforms 70 and 40kDa.
Western Blot: HDAC7 Antibody [NBP1-44092] - Lane 1: mouse brain homogenate. Lane 2: mouse brain homogenate blocked with peptide. Load: 4 ug per lane. Primary antibody: HDAC7 antibody at 1ug/mL for overnight at 4C. ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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HDAC7 Antibody Summary

Immunogen
HDAC7 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N terminal region of human HDAC7. (Uniprot: Q8WUI4)
Localization
Cell nucleus, Cytoplasm
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HDAC7
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:20000-1:60000
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Western Blot 1 ug/ml
Application Notes
Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~103kDa and isoforms at ~70, and 40 kDa corresponding to the appropriate cell lysate or extract.
Theoretical MW
102.93 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-44092 in the following applications:

Reactivity Notes

Cross-reactivity with HDAC7 from other sources has not been determined

Packaging, Storage & Formulations

Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Notes

This antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for HDAC7 Antibody

  • DKFZP586J0917
  • EC 3.5.1.98
  • HD7
  • HD7a
  • HDAC7
  • HDAC7A
  • HDAC7AFLJ99588
  • histone deacetylase 7
  • Histone deacetylase 7ADKFZp586J0917

Background

HDAC7 is a member of the class II mammalian histone deacetylases, which plays an important role in modulating the eukaryotic chromatin structure. Human HDAC7 is composed of 912 amino acid residues. Although HDAC7 is localized mostly to the cell nucleus, it is also found in the cytoplasm, suggesting nucleo-cytoplasmic shuttling. The histone deacetylase activity of HDAC7 maps to a carboxy-terminal domain and is dependent on interaction with class I HDACs in the nucleus. It is an active component of different transcriptional corepressor complexes that can be recruited to specific promoter regions via interactions with a growing number of sequence specific transcriptional factors. HDAC7 catalyzes removal of acetyl-groups from acetyl-lysines of histones and promotes compaction of chromatin in these regions, leading to the inhibition of gene transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: Flow, ICC/IF, IHC, IHC-P, IP, KO, WB
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Immunocytochemistry/Immunofluorescence: MEF2C Antibody [NBP2-17260] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MEF2C protein stained by MEF2C antibody diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.
Immunohistochemistry-Frozen: MEF2C Antibody [NBP2-17260] - Mouse cerebral cortex. Green: MEF2C stained by MEF2C antibody diluted at 1:250.Blue: Fluoroshield with DAPI.
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Deacetylase 8/HDAC8 A ...
NBP3-16713
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Immunohistochemistry-Paraffin: Histone Deacetylase 8/HDAC8 Antibody (1O2Y5) [NBP3-16713] - Immunohistochemistry of paraffin-embedded rat brain using Histone Deacetylase 8/HDAC8 Rabbit mAb (NBP3-16713) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: Histone Deacetylase 8/HDAC8 Antibody (1O2Y5) [NBP3-16713] - Immunohistochemistry of paraffin-embedded human liver cancer using Histone Deacetylase 8/HDAC8 Rabbit mAb (NBP3-16713) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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(OTI2G10)
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Immunohistochemistry: TCEAL1 Antibody (2G10) [NBP2-46085] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: TCEAL1 Antibody (2G10) [NBP2-46085] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Species: Hu
Applications: IHC, WB

Publications for HDAC7 Antibody (NBP1-44092) (0)

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Review for HDAC7 Antibody (NBP1-44092) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-44092:
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Western Blot HDAC7 NBP1-44092
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 Western Blot Human 09/26/2013
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Summary

ApplicationWestern Blot
Sample TestedHuman MCF-7 cell lysate
SpeciesHuman

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Western Blot HDAC7 NBP1-44092
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 Verified Customer
09/26/2013
Application: Western Blot
Species: Human
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Bioinformatics

Gene Symbol HDAC7
Entrez
Uniprot