Home » HDAC7 » HDAC7 Antibodies » HDAC7 Antibody

HDAC7 Antibody

 (1 Review)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western Blot: HDAC7 Antibody [NBP1-44092] - Lane 1: mouse brain homogenate. Lane 2: mouse brain homogenate blocked with peptide. Load: 4 ug per lane. Primary antibody: HDAC7 antibody at 1ug/mL for overnight at 4C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 103kDa/ 103, 70, and 40kDa for HDAC7. Other band(s): HDAC7 isoforms 70 and 40kDa.
Western Blot: HDAC7 Antibody [NBP1-44092] - Lane 1: mouse brain homogenate. Lane 2: mouse brain homogenate blocked with peptide. Load: 4 ug per lane. Primary antibody: HDAC7 antibody at 1ug/mL for overnight at 4C. ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.16 mg/ml

Order Details

100% Guarantee on every product

HDAC7 Antibody Summary

Immunogen
HDAC7 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N terminal region of human HDAC7.
Specificity
This antibody is specific to HDAC7
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HDAC7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • ELISA 1:20000 - 1:60000
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
Reviewed Applications
Read 1 Review rated 5
using
NBP1-44092 in the following applications:

Reactivity Notes

Cross reactivity with HDAC7 from other sources has not been tested.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
1.16 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HDAC7 Antibody

  • DKFZP586J0917
  • EC 3.5.1.98
  • HD7
  • HD7A
  • HDAC7AFLJ99588
  • histone deacetylase 7
  • Histone deacetylase 7ADKFZp586J0917

Background

HDAC7 is a member of the class II mammalian histone deacetylases, which plays an important role in modulating the eukaryotic chromatin structure. Human HDAC7 is composed of 912 amino acid residues. Although HDAC7 is localized mostly to the cell nucleus, it is also found in the cytoplasm, suggesting nucleo-cytoplasmic shuttling. The histone deacetylase activity of HDAC7 maps to a carboxy-terminal domain and is dependent on interaction with class I HDACs in the nucleus. It is an active component of different transcriptional corepressor complexes that can be recruited to specific promoter regions via interactions with a growing number of sequence specific transcriptional factors. HDAC7 catalyzes removal of acetyl-groups from acetyl-lysines of histones and promotes compaction of chromatin in these regions, leading to the inhibition of gene transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

HDAC9 Antibody
NBP2-03993
Western Blot: HDAC9 Antibody [NBP2-03993] - Analysis of HDAC-9 in human HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) and mouse RAW cell lysate using this antibody. 0 ug/ml, 1.0 ug/ml and 0.5 ug/ml respectively. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunohistochemistry-Paraffin: HDAC9 Antibody [NBP2-03993] - Analysis of HDAC-9 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top left) and NBP2-03993 (bottom left, right) at 5 ug/ml.
(1 Review)
Species: Hu, Bv, Ca, Pm
Applications: WB, IHC, IHC-P
Histone
Deacetylase 4/HDAC4 A
NBP2-22151
Western Blot: HDAC4 Antibody [NBP2-22151] - WB analysis of HDAC4 in NIH/3T3 lysate.
Immunocytochemistry/Immunofluorescence: Histone Deacetylase 4/HDAC4 Antibody [NBP2-22151] - HDAC4 antibody was tested in HeLa cells at a 1:100 dilution against Dylight 488 (Green) using a 40X objective. Alpha tubulin and nuclei were counterstained against Dylight 568 (Red) and DAPI (Blue), respectively.
Immunohistochemistry: HDAC4 Antibody [NBP2-22151] - IHC analysis of HDAC4 in mouse pancreas.
(3 Publications)
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
PKC mu Antibody
NBP1-87789
Immunocytochemistry/Immunofluorescence: PKC mu Antibody [NBP1-87789] - Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.
Immunohistochemistry-Paraffin: PKC mu Antibody [NBP1-87789] - Staining of human parathyroid gland shows cytoplasmic positivity in glandular cells.
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NGFI-B
alpha/Nur77/NR4A1 Ant
NB100-56745
Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745] - Analysis of Nur77 (Nak-1) in 15 ugs of NIH3T3 cell lysate using Nur77 antibody at 1:500 dilution
Immunocytochemistry/Immunofluorescence: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-NGFI-B alpha at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunocytochemistry/Immunofluorescence: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745] - NUR77 antibody was tested in Jurkat cells at a 1:50 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 568 (Red) and DAPI (Blue), respectively.
(11 Publications)
Species: Hu, Mu
Applications: WB, ChIP, ICC/IF, IP
HDAC5 Antibody
NBP2-22152
Western Blot: HDAC5 Antibody [NBP2-22152] - LNCaP cells with normal medium and with androgen depletion. Image from verified customer review.
Immunocytochemistry/Immunofluorescence: HDAC5 Antibody [NBP2-22152] - HDAC5 antibody was tested in A431 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry: HDAC5 Antibody [NBP2-22152] - IHC analysis of HDAC5 in mouse pancreas.
(1 Review)
(1 Publication)
Species: Hu, Mu, Pm
Applications: WB, ICC/IF, IHC, IHC-P
HDAC3 Antibody
NB100-1669
Western Blot: HDAC3 Antibody [NB100-1669] - Analysis of HDAC3 in HeLa cell lysate with anti-HDAC3 this antibody.
Immunocytochemistry/Immunofluorescence: HDAC3 Antibody [NB100-1669] - HDAC3 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei were counterstained with DAPI (blue). An antibody dilution of 1:10 was used. Image objective 40x.
Immunohistochemistry-Paraffin: HDAC3 Antibody [NB100-1669] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human colon cancer using 1:50 conc. of HDAC3 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining of HDAC3 was observed. Staining was performed by Histowiz.
(7 Publications)
Species: Hu
Applications: WB, Simple Western, ChIP, ICC/IF, IHC, IHC-P, IP
MEF2A Antibody
(JA33-04)
NBP2-66943
Western Blot: MEF2A Antibody (JA33-04) [NBP2-66943] - Analysis of MEF2A on rat spleen tissue lysate using anti-MEF2A antibody at 1/1,000 dilution.
Immunohistochemistry-Paraffin: MEF2A Antibody (JA33-04) [NBP2-66943] - Analysis of paraffin-embedded mouse spleen tissue using anti-MEF2A antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: MEF2A Antibody (JA33-04) [NBP2-66943] - Analysis of paraffin-embedded human spleen tissue using anti-MEF2A antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
Histone
Deacetylase 2/HDAC2 A ...
NBP2-67451
Western Blot: Histone Deacetylase 2/HDAC2 Antibody (SY29-02) [NBP2-67451] - Analysis of HDAC2 on different lysates using anti-HDAC2 antibody at 1/1,000 dilution. Positive control: Lane 1: 293T Lane 2: K562
Immunocytochemistry/Immunofluorescence: Histone Deacetylase 2/HDAC2 Antibody (SY29-02) [NBP2-67451] - Staining HDAC2 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Histone Deacetylase 2/HDAC2 Antibody (SY29-02) [NBP2-67451] - Analysis of paraffin-embedded rat spinal cord tissue using anti-HDAC2 antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
HDAC1 Antibody
NB100-56340
Western Blot: HDAC1 Antibody [NB100-56340] - analysis of HDAC1 in 293 cell lysate with this antibody.
Immunohistochemistry-Paraffin: HDAC1 Antibody [NB100-56340] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human testis using HDAC1 antibody at 1:250 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.
Simple Western: HDAC1 Antibody [NB100-56340] - Simple Western lane view shows a specific band for HDAC1 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
(2 Reviews)
(10 Publications)
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-Fr, IHC-P
p21/CIP1/CDKN1A
Antibody (WA-1 (HJ21)
NBP2-29463
Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (WA-1 (HJ21)) [NBP2-29463] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 Monoclonal Antibody (WA-1).
(11 Publications)
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
TIP60 Antibody
NBP2-24613
Western Blot: TIP60 Antibody [NBP2-24613] - analysis of TIP60 in human brain lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain lysate using this antibody. 25 ug/ml.
Immunohistochemistry-Paraffin: TIP60 Antibody [NBP2-24613] - analysis of TIP60 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.
Simple Western: TIP60 Antibody [NBP2-24613] - Simple Western lane view shows a specific band for TIP60 in 0.2 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
(1 Publication)
Species: Hu
Applications: WB, Simple Western, IHC-P
HIF-1 alpha
Antibody (H1alpha67)
NB100-105
Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha induction by CoCl2 on Caki-1 cell lysate. Image from verified customer review.
Knockout Validated: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha was detected in immersion fixed DFO treated Hela cells (left) but was not detected in HIF-1 knockout Hela cells (right) using Mouse Anti-human HIF-1 alpha monoclonal antibody (Catalog #NB100-105) at 25 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei.
Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Analysis using the HRP conjugate of NB100-105. Detection of 50ug cobalt chloride induced COS-7 nuclear extracts (NB800-PC26) using NB100-105.
(32 Reviews)
(742 Publications)
Species: Hu, Mu, Rt, Po, Bv, Ft, Mk, Pm, Rb, Sh, Xp
Applications: WB, ChIP, ELISA, Flow, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, TCS, KO, LA
TNF-alpha
[Unconjugated]
210-TA
Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
(21 Reviews)
(525 Publications)
Species: Hu
KAT3B/p300
Antibody (RW105)
NB100-616
Western Blot: KAT3B/p300 Antibody (RW105) [NB100-616] - p300 detected in a HeLa nuclear extract using NB 100-616 (1:250). ECL: 20 minute exposure.
Immunocytochemistry/Immunofluorescence: KAT3B/p300 Antibody (RW105) [NB100-616] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-KAT3B/p300 [RW105] at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Flow (Intracellular): KAT3B/p300 Antibody (RW105) [NB100-616] - An intracellular stain was performed on RAW 246.7 cells with KAT3B/p300 antibody (RW105) NB100-616PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.
(9 Publications)
Species: Hu, Mu, Rt, Md, Pm
Applications: WB, ChIP, EM, ICC/IF, IP, In vitro, Flow-IC
14-3-3 Antibody
NBP2-27202
Western Blot: 14-3-3 Antibody [NBP2-27202] - analysis of 14-3-3 using 14-3-3 antibody. Human Brain tissue lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain, and 4) rat brain lysate probed with 2 ug/ml of 14-3-3 antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Simple Western: 14-3-3 Antibody [NBP2-27202] - Simple Western lane view shows a specific band for 14-3-3 Gamma in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
(1 Review)
Species: Hu, Mu, Rt, Po, Ca, Ch, ChHa, Fe, Op, Pm
Applications: WB, Simple Western
CD4 Antibody
NBP1-19371
Western Blot: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in the following cell lysates: Lane 1) human spleen 2) human tonsil 3) human placenta 4) human kidney 5) human liver 6) mouse spleen 7) mouse placenta 8) rat placenta.
Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in Jurkat cells with Dylight 488 (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry-Frozen: CD4 Antibody [NBP1-19371] - Staining in mouse liver tissue.
(7 Reviews)
(14 Publications)
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
MEF2C Antibody
(OTI1H5)
NBP2-00493
Western Blot: MEF2C Antibody (1H5) [NBP2-00493] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MEF2C (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MEF2C.
Immunocytochemistry/Immunofluorescence: MEF2C Antibody (1H5) [NBP2-00493] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY MEF2C.
Immunohistochemistry-Paraffin: MEF2C Antibody (1H5) [NBP2-00493] - Staining of paraffin-embedded Human Ovary tissue using anti-MEF2C mouse monoclonal antibody.
(1 Publication)
Species: Hu, Mk
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Histone
Deacetylase 8/HDAC8 A ...
NBP2-67837
Western Blot: Histone Deacetylase 8/HDAC8 Antibody (JJ0845) [NBP2-67837] - Analysis of HDAC8 on different lysates using anti-HDAC8 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: K562
Immunocytochemistry/Immunofluorescence: Histone Deacetylase 8/HDAC8 Antibody (JJ0845) [NBP2-67837] - Staining HDAC8 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Histone Deacetylase 8/HDAC8 Antibody (JJ0845) [NBP2-67837] - Analysis of paraffin-embedded human pancreas tissue using anti-HDAC8 antibody. Counter stained with hematoxylin.
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
TCEAL1 Antibody
(OTI2G10)
NBP2-46085
Western Blot: TCEAL1 Antibody (2G10) [NBP2-46085] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TCEAL1.
Immunohistochemistry: TCEAL1 Antibody (2G10) [NBP2-46085] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(
Immunohistochemistry: TCEAL1 Antibody (2G10) [NBP2-46085] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Species: Hu
Applications: WB, IHC
HDAC7 Antibody
NBP1-44092
HDAC7 Antibody
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, ICC/IF, IHC, IHC-P

Publications for HDAC7 Antibody (NBP1-44092) (0)

There are no publications for HDAC7 Antibody (NBP1-44092).
By submitting your publication information earn gift cards and discounts for future purchases.

Review for HDAC7 Antibody (NBP1-44092) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-44092:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot HDAC7 NBP1-44092
Enlarge
reviewed by:
Corey.
 WB Human 09/26/2013
View

Summary

ApplicationWestern Blot
Sample TestedHuman MCF-7 cell lysate
SpeciesHuman

Blocking

Blocking Details5% NFDM

Primary Anitbody

Dilution Ratio1:500, 4C O/N

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HDAC7 Antibody (NBP1-44092) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for HDAC7 Antibody (NBP1-44092)

Discover related pathways, diseases and genes to HDAC7 Antibody (NBP1-44092). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for HDAC7 Antibody (NBP1-44092)

Discover more about diseases related to HDAC7 Antibody (NBP1-44092).
 

Pathways for HDAC7 Antibody (NBP1-44092)

View related products by pathway.

PTMs for HDAC7 Antibody (NBP1-44092)

Learn more about PTMs related to HDAC7 Antibody (NBP1-44092).
 

Research Areas for HDAC7 Antibody (NBP1-44092)

Find related products by research area.

Blogs on HDAC7

There are no specific blogs for HDAC7, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0
Western Blot HDAC7 NBP1-44092
Enlarge
 Corey.
09/26/2013
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol HDAC7
Entrez
Uniprot