Hemoglobin alpha Antibody (SR2950) - BSA Free

Immunohistochemistry-Paraffin: Hemoglobin alpha Antibody (SR2950) [NBP3-27727] - Immunohistochemical analysis of paraffin-embedded human spleen

Western Blot: Hemoglobin alpha Antibody (SR2950) [NBP3-27727] - Western blot analysis of Hemoglobin alpha expression in K562 cell lysate.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC, IP
• Clone: SR2950
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Hemoglobin alpha Antibody (SR2950) - BSA Free Summary

• Description: Novus Biologicals Rabbit Hemoglobin alpha Antibody (SR2950) - BSA Free (NBP3-27727) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human Hemoglobin alpha (Uniprot #: P69905)
• Specificity: Detects endogenous levels of Hemoglobin alpha
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HBA1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50
  • Immunohistochemistry 1:50-1:200
  • Immunoprecipitation 1:50
  • Western Blot 1:500-1:2000
• Theoretical MW: 15 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 150mM NaCl, 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Hemoglobin alpha Antibody (SR2950) - BSA Free

• alpha one globin
• alpha-1 globin
• alpha-1-globin
• alpha-globin
• CD31
• hemoglobin alpha 1 globin chain
• Hemoglobin alpha chain
• hemoglobin alpha-1 chain
• hemoglobin subunit alpha
• hemoglobin, alpha 1
• MGC126895
• MGC126897

Background

Hemoglobin is involved in oxygen transport from the lung to the various peripheral tissues.The alpha (HBA) and beta (HBB) loci determine the structure of the 2 types of polypeptide chains in adult Hemoglobin. The normal adult Hemoglobin tetramer consists of two alpha chains and two beta chains. Mutant beta globin causes sickle cell anemia. Absence of beta chain causes beta zero thalassemia. Reduced amounts of detectable beta globin causes beta plus thalassemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HBA1