![Immunocytochemistry/Immunofluorescence: Hemoglobin beta Antibody [NBP2-14081] - Staining of human cell line Hep G2 shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/Hemoglobin-beta-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-14081-img0004.jpg "Immunocytochemistry/Immunofluorescence: Hemoglobin beta Antibody [NBP2-14081] - Staining of human cell line Hep G2 shows localization to vesicles. Antibody staining is shown in green.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | ICC/IF, IHC, IHC-P |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: FATLSELHCDKLHVDPENFRLLGN |
| Predicted Species | Mouse (96%), Rat (96%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | HBB |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100. |
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| Reviewed Applications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
![Western Blot: Hemoglobin A2 Antibody [NBP3-05569] - Western blot analysis of extracts of mouse liver, using Hemoglobin A2 antibody (NBP3-05569) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/images2/Hemoglobin-A2-Antibody-Western-Blot-NBP3-05569-img0003.jpg)
![Immunohistochemistry: Hemoglobin A2 Antibody [NBP3-05569] - Immunofluorescence analysis of Human placenta using Hemoglobin A2 antibody (NBP3-05569) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/images2/Hemoglobin-A2-Antibody-Immunohistochemistry-NBP3-05569-img0004.jpg)
![Immunohistochemistry: Hemoglobin A2 Antibody [NBP3-05569] - Immunofluorescence analysis of Rat placenta using Hemoglobin A2 antibody (NBP3-05569) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/images2/Hemoglobin-A2-Antibody-Immunohistochemistry-NBP3-05569-img0005.jpg)
![Immunohistochemistry: GATA-1 Antibody (4F5) [NBP1-47492] - Staining of Fetal Baboon Liver. Antigen retrieval: 10mM sodium citrate, pH 6.0, Primary Ab: 1:500 dilution, Secondary Ab (biotinylated): 1:200, Streptavidin-AlexaFluor488: 1:500 (green), Quench autofluorescence with 1% Sudan Black in 70% methanol. Image from verified customer review.](http://images.novusbio.com/images2/GATA-1-Antibody-(4F5)-Immunohistochemistry-NBP1-47492-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: GATA-1 Antibody (4F5) [NBP1-47492] - Analysis of K562(left) cells using GATA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/images2/GATA-1-Antibody-(4F5)-Immunocytochemistry-Immunofluorescence-NBP1-47492-img0004.jpg)
![Immunohistochemistry-Paraffin: GATA-1 Antibody (4F5) [NBP1-47492] - Analysis of pancreatic cancer, using GATA1 mouse mAb with DAB staining.](http://images.novusbio.com/images2/GATA-1-Antibody-(4F5)-Immunohistochemistry-NBP1-47492-img0005.jpg)
![Immunohistochemistry-Paraffin: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-82580] - Staining of human bone marrow shows moderate to strong nuclear positivity in hematopoietic cells.](http://images.novusbio.com/images2/Nuclear-Factor-Erythroid-Derived-2-Antibody-Immunohistochemistry-Paraffin-NBP1-82580-img0014.jpg)
![Immunohistochemistry-Paraffin: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-82580] - Staining of human liver shows no nuclear positivity in hepatocytes as expected.](http://images.novusbio.com/images2/Nuclear-Factor-Erythroid-Derived-2-Antibody-Immunohistochemistry-Paraffin-NBP1-82580-img0015.jpg)
![Immunohistochemistry-Paraffin: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-82580] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/images2/Nuclear-Factor-Erythroid-Derived-2-Antibody-Immunohistochemistry-Paraffin-NBP1-82580-img0016.jpg)
![ELISA: KLF1 Antibody (1B6A3) [NBP2-37380] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/images2/KLF1-Antibody-(1B6A3)-ELISA-NBP2-37380-img0001.jpg)
![Western Blot: KLF1 Antibody (1B6A3) [NBP2-37380] - Analysis using KLF1 mAb against human KLF1 recombinant protein. (Expected MW is 42.6 kDa)](http://images.novusbio.com/images2/KLF1-Antibody-(1B6A3)-Western-Blot-NBP2-37380-img0002.jpg)
![Flow Cytometry: KLF1 Antibody (1B6A3) [NBP2-37380] - Analysis of HeLa cells using KLF1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/images2/KLF1-Antibody-(1B6A3)-Flow-Cytometry-NBP2-37380-img0003.jpg)
![Western Blot: Hemoglobin epsilon Antibody [NBP2-43557] - Human tissue extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin epsilon antibody [N1N2], N-term diluted at 1:500.](http://images.novusbio.com/images2/Hemoglobin-epsilon-Antibody-Western-Blot-NBP2-43557-img0001.jpg)
![Western Blot: Hemoglobin epsilon Antibody [NBP2-43557] - Untreated (-) and treated (+) mouse tissue extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin epsilon antibody [N1N2], N-term. (CFA = Complete Freund's adjuvant)](http://images.novusbio.com/images2/Hemoglobin-epsilon-Antibody-Western-Blot-NBP2-43557-img0003.jpg)
![Western Blot: Hemoglobin epsilon Antibody [NBP2-43557] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin epsilon antibody [N1N2], N-term diluted at 1:1000.](http://images.novusbio.com/images2/Hemoglobin-epsilon-Antibody-Western-Blot-NBP2-43557-img0004.jpg)
![Immunohistochemistry: Spectrin beta 1 Antibody (4C3) [NB300-574] - Neu5Gc co-staining with beta spectrin, CD11b, CD8 or Pax7 in normal, BMD or DMD human muscle. Otherwise normal, Becker muscular dystrophy (BMD) and Duchenne muscular dystrophy (DMD) muscle biopsy sections were stained for Neu5Gc (green),Spectrin beta 1 (red) and DAPI (blue). Arrows indicate Neu5Gc puncta in cytoplasmic or perimembranous regions of BMD and DMD skeletal myofibers. Bar is 50 um for all panels. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0088226) licensed under a CC-BY licence.](http://images.novusbio.com/images2/Spectrin-beta-1-Antibody-(4C3)-Immunohistochemistry-NB300-574-img0010.jpg)
![Flow Cytometry: Spectrin beta 1 Antibody (4C3) [NB300-574] - Analysis of SH-SY5Y cells compared to an isotype control (blue).](http://images.novusbio.com/images2/Spectrin-beta-1-Antibody-(4C3)-Flow-Cytometry-NB300-574-img0001.jpg)
![Flow Cytometry: Spectrin beta 1 Antibody (4C3) [NB300-574] - Analysis of NIH/3T3 cells compared to an isotype control (blue).](http://images.novusbio.com/images2/Spectrin-beta-1-Antibody-(4C3)-Flow-Cytometry-NB300-574-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: ZCCHC6 Antibody [NBP1-82257] - Staining of human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm.](http://images.novusbio.com/images2/ZCCHC6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82257-img0005.jpg)
![Immunohistochemistry-Paraffin: ZCCHC6 Antibody [NBP1-82257] - Staining of human skeletal muscle shows cytoplasmic positivity in myocytes.](http://images.novusbio.com/images2/ZCCHC6-Antibody-Immunohistochemistry-Paraffin-NBP1-82257-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: ZCCHC6 Antibody [NBP1-82257] - Staining of human cell line A-431 shows localization to cytosol.](http://images.novusbio.com/images2/ZCCHC6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82257-img0007.jpg)
![Western Blot: GPHA2 Antibody [NBP3-17255] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: RT4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: Tonsil](http://images.novusbio.com/images2/GPHA2-Antibody-Western-Blot-NBP3-17255-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: GPHA2 Antibody [NBP3-17255] - Staining of human cell line Hep G2 shows localization to vesicles.](http://images.novusbio.com/images2/GPHA2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-17255-img0001.jpg)
![Western Blot: ATP6V0A2 Antibody [NBP1-59069] - Hela tissue lysate at a concentration of 1ug/ml.](http://images.novusbio.com/images2/ATP6V0A2-Antibody-Western-Blot-NBP1-59069-img0001.jpg)
![Western Blot: ATP6V0A2 Antibody [NBP1-59069] - HeLa cells, concentration 3.3 ug/ml.](http://images.novusbio.com/images2/ATP6V0A2-Antibody-Western-Blot-NBP1-59069-img0002.jpg)
![Immunohistochemistry-Paraffin: ATP6V0A2 Antibody [NBP1-59069] - overexpressed mouse sequences tissue](http://images.novusbio.com/images2/ATP6V0A2-Antibody-Immunohistochemistry-Paraffin-NBP1-59069-img0005.jpg)
![Flow Cytometry: c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on U-937 cells with c-Myc Antibody (9E10) NB600-302AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/images2/c-Myc-Antibody-(9E10)-Flow-Cytometry-NB600-302-img0014.jpg)
![Flow Cytometry: c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on Jurkat cells with c-Myc Antibody (9E10) NB600-302R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.](http://images.novusbio.com/images2/c-Myc-Antibody-(9E10)-Flow-Cytometry-NB600-302-img0019.jpg)
![Western Blot: c-Myc Antibody (9E10) [NB600-302] - CtIP is phosphorylated by CDKs at multiple sites. Myc-BRCA1 and/or HA-CtIP WT or indicated mutants were expressed in 293T cells and co-immunoprecipitation was performed. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pgen.1003277) licensed under a CC-BY licence.](http://images.novusbio.com/images2/c-Myc-Antibody-(9E10)-Western-Blot-NB600-302-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: Rpn2 Antibody [NBP2-38323] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & actin filaments.](http://images.novusbio.com/images2/Rpn2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38323-img0004.jpg)
![Immunohistochemistry-Paraffin: Rpn2 Antibody [NBP2-38323] - Staining of human cerebral cortex, kidney, liver and testis using Anti-PSMD1 antibody NBP2-38323 (A) shows similar protein distribution across tissues to independent antibody NBP2-38324 (B).](http://images.novusbio.com/images2/Rpn2-Antibody-Immunohistochemistry-Paraffin-NBP2-38323-img0005.jpg)
![Immunohistochemistry-Paraffin: Rpn2 Antibody [NBP2-38323] - Staining of human testis.](http://images.novusbio.com/images2/Rpn2-Antibody-Immunohistochemistry-Paraffin-NBP2-38323-img0006.jpg)
| Images | Ratings | Applications | Species | Date | Details | ||||||
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reviewed by:
Verified Customer |
Immunohistochemistry | Mouse | 04/28/2017 |
Summary
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Secondary Antibodies |
Isotype Controls |
PTMs for Hemoglobin beta Antibody (NBP2-14081)Learn more about PTMs related to Hemoglobin beta Antibody (NBP2-14081).
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| Verified Customer 04/28/2017 |
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| Application: | Immunohistochemistry | |
| Species: | Mouse |
| Gene Symbol | HBB |
![Immunohistochemistry-Paraffin: Hemoglobin beta Antibody [NBP2-14081] - Staining of human placenta shows erythrocyte positivity in vessels.](http://images.novusbio.com/fullsize2/Hemoglobin-beta-Antibody-Immunohistochemistry-Paraffin-NBP2-14081-img0003.jpg "Immunohistochemistry-Paraffin: Hemoglobin beta Antibody [NBP2-14081] - Staining of human placenta shows erythrocyte positivity in vessels.")
![Immunohistochemistry-Paraffin: DNASE1 Antibody [NBP1-84999] - Analysis in human duodenum and liver tissues using Anti-DNASE1 antibody. Corresponding DNASE1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/DNASE1-Antibody-Immunohistochemistry-Paraffin-NBP1-84999-img0011.jpg)
![Western Blot: DNASE1 Antibody [NBP1-84999] - Analysis in control (vector only transfected HEK293T lysate) and DNASE1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/images2/DNASE1-Antibody-Western-Blot-NBP1-84999-img0006.jpg)
![Immunohistochemistry-Paraffin: DNASE1 Antibody [NBP1-84999] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/images2/DNASE1-Antibody-Immunohistochemistry-Paraffin-NBP1-84999-img0013.jpg)
![Immunohistochemistry-Paraffin: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Analysis of paraffin-embedded mouse heart tissue using anti-alpha sarcoglycan antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/alpha-Sarcoglycan-Antibody-(JA51-81)-Immunohistochemistry-Paraffin-NBP2-67150-img0001.jpg)
![Immunohistochemistry-Paraffin: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Analysis of paraffin-embedded mouse skeletal muscle tissue using anti-alpha sarcoglycan antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/alpha-Sarcoglycan-Antibody-(JA51-81)-Immunohistochemistry-Paraffin-NBP2-67150-img0002.jpg)
![Immunohistochemistry-Frozen: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Human skeletal muscle tissue. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/images2/alpha-Sarcoglycan-Antibody-(JA51-81)-Immunohistochemistry-Frozen-NBP2-67150-img0005.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](http://images.novusbio.com/images2/Goat-anti-Rabbit-IgG-(H+L)-Secondary-Antibody-[HRP]-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](http://images.novusbio.com/images2/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")