![Immunocytochemistry/Immunofluorescence: Hemoglobin beta Antibody [NBP2-14081] - Staining of human cell line Hep G2 shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/Hemoglobin-beta-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-14081-img0004.jpg "Immunocytochemistry/Immunofluorescence: Hemoglobin beta Antibody [NBP2-14081] - Staining of human cell line Hep G2 shows localization to vesicles. Antibody staining is shown in green.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | ICC/IF, IHC, IHC-P |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: FATLSELHCDKLHVDPENFRLLGN |
| Specificity | Specificity of human Hemoglobin beta antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins. |
| Predicted Species | Mouse (96%), Rat (96%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | HBB |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100. |
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| Reviewed Applications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||
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reviewed by:
Sitara Sankar |
IHC | Mouse | 04/28/2017 |
Summary
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Secondary Antibodies |
Isotype Controls |
PTMs for Hemoglobin beta Antibody (NBP2-14081)Learn more about PTMs related to Hemoglobin beta Antibody (NBP2-14081).
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| Gene Symbol | HBB |
![Immunohistochemistry-Paraffin: Hemoglobin beta Antibody [NBP2-14081] - Staining of human placenta shows erythrocyte positivity in vessels.](http://images.novusbio.com/fullsize2/Hemoglobin-beta-Antibody-Immunohistochemistry-Paraffin-NBP2-14081-img0003.jpg "Immunohistochemistry-Paraffin: Hemoglobin beta Antibody [NBP2-14081] - Staining of human placenta shows erythrocyte positivity in vessels.")
![Western Blot: GATA-1 Antibody (4F5) [NBP1-47492] - Analysis using GATA1 mouse mAb against K562 (1) cell lysate.](http://images.novusbio.com/images2/GATA-1-Antibody-(4F5)-Western-Blot-NBP1-47492-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: GATA-1 Antibody (4F5) [NBP1-47492] - Analysis of K562(left) cells using GATA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/images2/GATA-1-Antibody-(4F5)-Immunocytochemistry-Immunofluorescence-NBP1-47492-img0004.jpg)
![Immunohistochemistry: GATA-1 Antibody (4F5) [NBP1-47492] - Staining of Fetal Baboon Liver. Antigen retrieval: 10mM sodium citrate, pH 6.0, Primary Ab: 1:500 dilution, Secondary Ab (biotinylated): 1:200, Streptavidin-AlexaFluor488: 1:500 (green), Quench autofluorescence with 1% Sudan Black in 70% methanol. Image from verified customer review.](http://images.novusbio.com/images2/GATA-1-Antibody-(4F5)-Immunohistochemistry-NBP1-47492-img0007.jpg)
![Immunohistochemistry-Paraffin: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-82580] - Staining in human bone marrow and liver tissues. Corresponding NFE2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/Nuclear-Factor-Erythroid-Derived-2-Antibody-Immunohistochemistry-Paraffin-NBP1-82580-img0017.jpg)
![Western Blot: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-82580] - Analysis in control (vector only transfected HEK293T lysate) and NFE2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/images2/Nuclear-Factor-Erythroid-Derived-2-Antibody-Western-Blot-NBP1-82580-img0019.jpg)
![Western Blot: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-82580] - Analysis in human cell line HEL.](http://images.novusbio.com/images2/Nuclear-Factor-Erythroid-Derived-2-Antibody-Western-Blot-NBP1-82580-img0018.jpg)
![Western Blot: KLF1 Antibody (1B6A3) [NBP2-37380] - Western blot analysis using KLF1 mAb against human KLF1 recombinant protein. (Expected MW is 42.6 kDa)](http://images.novusbio.com/images2/KLF1-Antibody-(1B6A3)-Western-Blot-NBP2-37380-img0002.jpg)
![Immunohistochemistry: KLF1 Antibody (1B6A3) [NBP2-37380] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using KLF1 mouse mAb with DAB staining.](http://images.novusbio.com/images2/KLF1-Antibody-(1B6A3)-Immunohistochemistry-NBP2-37380-img0005.jpg)
![Flow Cytometry: KLF1 Antibody (1B6A3) [NBP2-37380] - Flow cytometric analysis of HeLa cells using KLF1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/images2/KLF1-Antibody-(1B6A3)-Flow-Cytometry-NBP2-37380-img0003.jpg)
![Western Blot: DNASE1 Antibody [NBP1-84999] - Analysis in control (vector only transfected HEK293T lysate) and DNASE1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/images2/DNASE1-Antibody-Western-Blot-NBP1-84999-img0006.jpg)
![Immunohistochemistry-Paraffin: DNASE1 Antibody [NBP1-84999] - Staining of human duodenum shows high expression.](http://images.novusbio.com/images2/DNASE1-Antibody-Immunohistochemistry-Paraffin-NBP1-84999-img0015.jpg)
![Immunohistochemistry-Paraffin: DNASE1 Antibody [NBP1-84999] - Analysis in human duodenum and liver tissues using Anti-DNASE1 antibody. Corresponding DNASE1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/DNASE1-Antibody-Immunohistochemistry-Paraffin-NBP1-84999-img0011.jpg)
![Western Blot: Hemoglobin epsilon Antibody [NBP2-43557] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin epsilon antibody [N1N2], N-term diluted at 1:1000.](http://images.novusbio.com/images2/Hemoglobin-epsilon-Antibody-Western-Blot-NBP2-43557-img0004.jpg)
![Western Blot: Hemoglobin epsilon Antibody [NBP2-43557] - Human tissue extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin epsilon antibody [N1N2], N-term diluted at 1:500.](http://images.novusbio.com/images2/Hemoglobin-epsilon-Antibody-Western-Blot-NBP2-43557-img0001.jpg)
![Western Blot: Hemoglobin epsilon Antibody [NBP2-43557] - Untreated (-) and treated (+) mouse tissue extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin epsilon antibody [N1N2], N-term. (CFA = Complete Freund's adjuvant)](http://images.novusbio.com/images2/Hemoglobin-epsilon-Antibody-Western-Blot-NBP2-43557-img0003.jpg)
![Western Blot: Spectrin beta 1 Antibody (4C3) [NB300-574] - Analysis of 25 ug of Hela cell lysates.](http://images.novusbio.com/images2/Spectrin beta 1 Antibody (4C3)-Western Blot-NB300-574-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Spectrin beta 1 Antibody (4C3) [NB300-574] - Analysis of Spectrin beta-1 (green) showing staining in the cytoplasm of A431 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/images2/Spectrin-beta-1-Antibody-(4C3)-Immunocytochemistry-Immunofluorescence-NB300-574-img0005.jpg)
![Immunohistochemistry: Spectrin beta 1 Antibody (4C3) [NB300-574] - Neu5Gc co-staining with beta spectrin, CD11b, CD8 or Pax7 in normal, BMD or DMD human muscle. Otherwise normal, Becker muscular dystrophy (BMD) and Duchenne muscular dystrophy (DMD) muscle biopsy sections were stained for Neu5Gc (green),Spectrin beta 1 (red) and DAPI (blue). Arrows indicate Neu5Gc puncta in cytoplasmic or perimembranous regions of BMD and DMD skeletal myofibers. Bar is 50 um for all panels. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0088226) licensed under a CC-BY licence.](http://images.novusbio.com/images2/Spectrin-beta-1-Antibody-(4C3)-Immunohistochemistry-NB300-574-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: ZCCHC6 Antibody [NBP1-82257] - Staining of human cell line A-431 shows localization to cytosol.](http://images.novusbio.com/images2/ZCCHC6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82257-img0007.jpg)
![Immunohistochemistry-Paraffin: ZCCHC6 Antibody [NBP1-82257] - Staining of human skeletal muscle shows cytoplasmic positivity in myocytes.](http://images.novusbio.com/images2/ZCCHC6-Antibody-Immunohistochemistry-Paraffin-NBP1-82257-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: ZCCHC6 Antibody [NBP1-82257] - Staining of human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm.](http://images.novusbio.com/images2/ZCCHC6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82257-img0005.jpg)
![Western Blot: GPHA2 Antibody [NBP3-04883] - Analysis of extracts of rat brain, using GPHA2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit.](http://images.novusbio.com/images2/GPHA2 Antibody-Western Blot-NBP3-04883-img0001.jpg)
![Western Blot: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Analysis of alpha sarcoglycan on rat heart tissue lysates using anti-alpha sarcoglycan antibody at 1:500 dilution.](http://images.novusbio.com/images2/alpha-Sarcoglycan Antibody (JA51-81)-Western Blot-NBP2-67150-img0003.jpg)
![Immunohistochemistry-Frozen: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Human skeletal muscle tissue. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/images2/alpha-Sarcoglycan-Antibody-(JA51-81)-Immunohistochemistry-Frozen-NBP2-67150-img0005.jpg)
![Immunohistochemistry-Paraffin: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Analysis of paraffin-embedded mouse heart tissue using anti-alpha sarcoglycan antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/alpha-Sarcoglycan-Antibody-(JA51-81)-Immunohistochemistry-Paraffin-NBP2-67150-img0001.jpg)
![Western Blot: ATP6V0A2 Antibody [NBP1-59069] - HeLa cells, concentration 3.3 ug/ml.](http://images.novusbio.com/images2/ATP6V0A2-Antibody-Western-Blot-NBP1-59069-img0002.jpg)
![Immunohistochemistry: ATP6V0A2 Antibody [NBP1-59069] - Human kidney lysate tissue at an antibody concentration of 5.0 ug/ml using anti-ATP6V0A2 antibody](http://images.novusbio.com/images2/ATP6V0A2-Antibody-Immunohistochemistry-NBP1-59069-img0008.jpg)
![Western Blot: ATP6V0A2 Antibody [NBP1-59069] - Hela tissue lysate at a concentration of 1ug/ml.](http://images.novusbio.com/images2/ATP6V0A2-Antibody-Western-Blot-NBP1-59069-img0001.jpg)
![Western Blot: c-Myc Antibody (9E10) [NB600-302] - CtIP is phosphorylated by CDKs at multiple sites. Myc-BRCA1 and/or HA-CtIP WT or indicated mutants were expressed in 293T cells and co-immunoprecipitation was performed. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pgen.1003277) licensed under a CC-BY licence.](http://images.novusbio.com/images2/c-Myc-Antibody-(9E10)-Western-Blot-NB600-302-img0022.jpg)
![Immunohistochemistry-Paraffin: c-Myc Antibody (9E10) [NB600-302] - c-Myc was detected in immersion fixed paraffin-embedded sections of human breast cancer using anti-human mouse monoclonal antibody (Catalog # NB600-302, clone 9E10) at 1:50 dilution overnight at 4 C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/images2/c-Myc-Antibody-(9E10)-Immunohistochemistry-Paraffin-NB600-302-img0009.jpg)
![Simple Western: c-Myc Antibody (9E10) [NB600-302] - Simple Western lane view shows a specific band for c-Myc in 0.5 mg/ml of Jurkat lysate. This experiment as performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/images2/c-Myc-Antibody-(9E10)-Simple-Western-NB600-302-img0006.jpg)
![Western Blot: Rpn2 Antibody [NBP2-38323] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/images2/Rpn2-Antibody-Western-Blot-NBP2-38323-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Rpn2 Antibody [NBP2-38323] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & actin filaments.](http://images.novusbio.com/images2/Rpn2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38323-img0004.jpg)
![Immunohistochemistry-Paraffin: Rpn2 Antibody [NBP2-38323] - Staining of human liver.](http://images.novusbio.com/images2/Rpn2-Antibody-Immunohistochemistry-Paraffin-NBP2-38323-img0008.jpg)
![Western Blot: Hemoglobin gamma-2 chain Antibody [NBP2-16806] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin gamma-2 chain antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/images2/Hemoglobin-gamma-2-chain-Antibody-Western-Blot-NBP2-16806-img0002.jpg)
![Western Blot: Hemoglobin gamma-2 chain Antibody [NBP2-16806] - Sample (30 ug of whole cell lysate) A: U87-MG 15% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/images2/Hemoglobin gamma-2 chain Antibody-Western Blot-NBP2-16806-img0001.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")