![Immunocytochemistry/Immunofluorescence: Hemoglobin beta Antibody [NBP2-14081] - Staining of human cell line Hep G2 shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/Hemoglobin-beta-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-14081-img0004.jpg "Immunocytochemistry/Immunofluorescence: Hemoglobin beta Antibody [NBP2-14081] - Staining of human cell line Hep G2 shows localization to vesicles. Antibody staining is shown in green.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | ICC/IF, IHC, IHC-P |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: FATLSELHCDKLHVDPENFRLLGN |
| Specificity | Specificity of human Hemoglobin beta antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins. |
| Predicted Species | Mouse (96%), Rat (96%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | HBB |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. |
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| Reviewed Applications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||
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reviewed by:
Sitara Sankar |
IHC | Mouse | 04/28/2017 |
Summary
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Secondary Antibodies |
Isotype Controls |
PTMs for Hemoglobin beta Antibody (NBP2-14081)Learn more about PTMs related to Hemoglobin beta Antibody (NBP2-14081).
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| Gene Symbol | HBB |
![Immunohistochemistry-Paraffin: Hemoglobin beta Antibody [NBP2-14081] - Staining of human placenta shows erythrocyte positivity in vessels.](http://images.novusbio.com/fullsize2/Hemoglobin-beta-Antibody-Immunohistochemistry-Paraffin-NBP2-14081-img0003.jpg "Immunohistochemistry-Paraffin: Hemoglobin beta Antibody [NBP2-14081] - Staining of human placenta shows erythrocyte positivity in vessels.")
![SDS-Page: Recombinant Human Hemoglobin A2 Protein [NBP2-23111]](http://images.novusbio.com/fullsize2/Recombinant-Human-Hemoglobin-A2-Protein-SDS-Page-NBP2-23111-img0002.jpg)
![Western Blot: GATA-1 Antibody (4F5) [NBP1-47492] - Western blot analysis using GATA1 mouse mAb against K562 (1) cell lysate.](http://images.novusbio.com/fullsize2/GATA-1-Antibody-(4F5)-Western-Blot-NBP1-47492-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: GATA-1 Antibody (4F5) [NBP1-47492] - Immunofluorescence analysis of K562(left) cells using GATA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize2/GATA-1-Antibody-(4F5)-Immunocytochemistry-Immunofluorescence-NBP1-47492-img0004.jpg)
![Immunohistochemistry: GATA-1 Antibody (4F5) [NBP1-47492] - Immunohistochemical analysis of paraffin-embedded pancreatic cancer, using GATA1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize2/GATA-1-Antibody-(4F5)-Immunohistochemistry-NBP1-47492-img0005.jpg)
![Western Blot: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-82580] - Analysis in human cell line HEL.](http://images.novusbio.com/fullsize2/Nuclear-Factor-Erythroid-Derived-2-Antibody-Western-Blot-NBP1-82580-img0018.jpg)
![Immunohistochemistry-Paraffin: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-82580] - Staining in human bone marrow and liver tissues. Corresponding NFE2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/Nuclear-Factor-Erythroid-Derived-2-Antibody-Immunohistochemistry-Paraffin-NBP1-82580-img0017.jpg)
![Immunohistochemistry-Paraffin: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-82580] - Staining of human bone marrow shows moderate to strong nuclear positivity in hematopoietic cells.](http://images.novusbio.com/fullsize2/Nuclear-Factor-Erythroid-Derived-2-Antibody-Immunohistochemistry-Paraffin-NBP1-82580-img0014.jpg)
![Western Blot: KLF1 Antibody (1B6A3) [NBP2-37380] - Western blot analysis using KLF1 mAb against human KLF1 recombinant protein. (Expected MW is 42.6 kDa)](http://images.novusbio.com/fullsize2/KLF1-Antibody-(1B6A3)-Western-Blot-NBP2-37380-img0002.jpg)
![Immunohistochemistry: KLF1 Antibody (1B6A3) [NBP2-37380] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using KLF1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize2/KLF1-Antibody-(1B6A3)-Immunohistochemistry-NBP2-37380-img0005.jpg)
![Flow Cytometry: KLF1 Antibody (1B6A3) [NBP2-37380] - Flow cytometric analysis of HeLa cells using KLF1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize2/KLF1-Antibody-(1B6A3)-Flow-Cytometry-NBP2-37380-img0003.jpg)
![Western Blot: DNASE1 Antibody [NBP1-84999] - Analysis in control (vector only transfected HEK293T lysate) and DNASE1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize2/DNASE1-Antibody-Western-Blot-NBP1-84999-img0006.jpg)
![Immunohistochemistry-Paraffin: DNASE1 Antibody [NBP1-84999] - Staining in human duodenum and liver tissues using anti-DNASE1 antibody. Corresponding DNASE1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/DNASE1-Antibody-Immunohistochemistry-Paraffin-NBP1-84999-img0014.jpg)
![Immunohistochemistry-Paraffin: DNASE1 Antibody [NBP1-84999] - Analysis in human duodenum and liver tissues using Anti-DNASE1 antibody. Corresponding DNASE1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/DNASE1-Antibody-Immunohistochemistry-Paraffin-NBP1-84999-img0011.jpg)
![Western Blot: Hemoglobin epsilon Antibody [NBP2-43557] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin epsilon antibody [N1N2], N-term diluted at 1:1000.](http://images.novusbio.com/fullsize2/Hemoglobin-epsilon-Antibody-Western-Blot-NBP2-43557-img0004.jpg)
![Western Blot: Hemoglobin epsilon Antibody [NBP2-43557] - Human tissue extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin epsilon antibody [N1N2], N-term diluted at 1:500.](http://images.novusbio.com/fullsize2/Hemoglobin-epsilon-Antibody-Western-Blot-NBP2-43557-img0001.jpg)
![Western Blot: Hemoglobin epsilon Antibody [NBP2-43557] - Untreated (-) and treated (+) mouse tissue extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin epsilon antibody [N1N2], N-term. (CFA = Complete Freund's adjuvant)](http://images.novusbio.com/fullsize2/Hemoglobin-epsilon-Antibody-Western-Blot-NBP2-43557-img0003.jpg)
![Western Blot: Spectrin beta 1 Antibody (4C3) [NB300-574] - Analysis of 25 ug of Hela cell lysates.](http://images.novusbio.com/fullsize2/Spectrin beta 1 Antibody (4C3)-Western Blot-NB300-574-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Spectrin beta 1 Antibody (4C3) [NB300-574] - Analysis of Spectrin beta-1 (green) showing staining in the cytoplasm of A431 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize2/Spectrin-beta-1-Antibody-(4C3)-Immunocytochemistry-Immunofluorescence-NB300-574-img0005.jpg)
![Immunohistochemistry-Paraffin: Spectrin beta 1 Antibody (4C3) [NB300-574] - Analysis showing positive staining in the cytoplasm and membrane of Mouse skeletal muscle (right) compared with a negative control in the absence of primary antibody (left).](http://images.novusbio.com/fullsize2/Spectrin-beta-1-Antibody-(4C3)-Immunohistochemistry-Paraffin-NB300-574-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ZCCHC6 Antibody [NBP1-82257] - Staining of human cell line A-431 shows localization to cytosol.](http://images.novusbio.com/fullsize2/ZCCHC6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82257-img0007.jpg)
![Immunohistochemistry-Paraffin: ZCCHC6 Antibody [NBP1-82257] - Staining of human skeletal muscle shows cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize2/ZCCHC6-Antibody-Immunohistochemistry-Paraffin-NBP1-82257-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: ZCCHC6 Antibody [NBP1-82257] - Staining of human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm.](http://images.novusbio.com/fullsize2/ZCCHC6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82257-img0005.jpg)
![Western Blot: GPHA2 Overexpression Lysate (Adult Normal) [NBL1-11228] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GPHA2.](http://images.novusbio.com/fullsize/GPHA2 Overexpression Lysate (Adult Normal)-Western Blot-NBL1-11228-img0002.jpg)
![Western Blot: alpha Sarcoglycan Antibody [NBP1-90306] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize2/alpha Sarcoglycan Antibody-Western Blot-NBP1-90306-img0004.jpg)
![Immunohistochemistry-Paraffin: alpha Sarcoglycan Antibody [NBP1-90306] - Staining of human heart muscle shows distinct positivity of intercalated discs in myocytes.](http://images.novusbio.com/fullsize2/alpha-Sarcoglycan-Antibody-Immunohistochemistry-Paraffin-NBP1-90306-img0001.jpg)
![Western Blot: ATP6V0A2 Antibody [NBP1-59069] - HeLa cells, concentration 3.3 ug/ml.](http://images.novusbio.com/fullsize2/ATP6V0A2-Antibody-Western-Blot-NBP1-59069-img0002.jpg)
![Immunohistochemistry: ATP6V0A2 Antibody [NBP1-59069] - Human kidney lysate tissue at an antibody concentration of 5.0 ug/ml using anti-ATP6V0A2 antibody](http://images.novusbio.com/fullsize2/ATP6V0A2-Antibody-Immunohistochemistry-NBP1-59069-img0008.jpg)
![Western Blot: ATP6V0A2 Antibody [NBP1-59069] - Hela tissue lysate at a concentration of 1ug/ml.](http://images.novusbio.com/fullsize2/ATP6V0A2-Antibody-Western-Blot-NBP1-59069-img0001.jpg)
![Western Blot: c-Myc Antibody [NB600-335] - Detection of c-myc-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-c-myc antibody NB600-335 used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize2/c-Myc-Antibody-Western-Blot-NB600-335-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: c-Myc Antibody [NB600-335] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-c-Myc conjugated to Alexa Fluor 488 [NB600-335AF488] at 10ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the Alexa Fluor 488 version of this antibody.](http://images.novusbio.com/fullsize2/c-Myc Antibody-Immunocytochemistry-Immunofluorescence-NB600-335-img0007.jpg)
![Western Blot: c-Myc Antibody [NB600-335] - Analysis using the HRP conjugate of NB600-335. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at 0.4 ug/ml (1:25,000).](http://images.novusbio.com/fullsize2/c-Myc-Antibody-Western-Blot-NB600-335-img0005.jpg)
![Western Blot: Rpn2 Antibody [NB120-2941] - Analysis of proteasome 19S subunit S1 from HeLa cell extract.](http://images.novusbio.com/fullsize2/Rpn2 Antibody-Western Blot-NB120-2941-img0003.jpg)
![Western Blot: Hemoglobin gamma-2 chain Antibody [NBP1-98291] - Sample Type: Human Fetal Liver Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize2/Hemoglobin-gamma-2-chain-Antibody-Western-Blot-NBP1-98291-img0003.jpg)
![Western Blot: Hemoglobin gamma-2 chain Antibody [NBP1-98291] - Human Fetal Kidney Lysate 1.0ug/ml, Gel Concentration: 10-20%](http://images.novusbio.com/fullsize2/Hemoglobin-gamma-2-chain-Antibody-Western-Blot-NBP1-98291-img0002.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")