HDAC7 Antibody

Western Blot: HDAC7 Antibody [NB100-61587] - Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibody used for WB at 0.4 ug/ml.

Immunohistochemistry-Paraffin: HDAC7 Antibody [NB100-61587] - Human prostate carcinoma (left) and mouse colon carcinoma (right). Antibody used at a dilution of 1:5,000.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IHC-P, IP (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

HDAC7 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 902 and 952 of human histone deacetylase 7A using the numbering given in entry NP_056216.1 (GeneID 51564).
• Predicted Species: Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HDAC7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin 1:1000 - 1:5000
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Immunoprecipitation Not recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HDAC7 Antibody

• DKFZP586J0917
• EC 3.5.1.98
• HD7
• HD7a
• HDAC7
• HDAC7A
• HDAC7AFLJ99588
• histone deacetylase 7
• Histone deacetylase 7ADKFZp586J0917

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to mouse HDAC7 gene whose protein promotes repression mediated via transcriptional corepressor SMRT. Multiple alternatively spliced transcript variants encoding several isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HDAC7 Antibody (NB100-61587)(2)

Publications using NB100-61587

• Yano M, Yasuda M, Sakaki M, Nagata K. Association of histone deacetylase expression with histology and prognosis of ovarian cancer. Oncol Lett. 2018-03-01 [PMID: 29456726] (IHC-P, Human)
• Murata H, Yoshimoto K, Hatae R et al. Detection of proneural/mesenchymal marker expression in glioblastoma: temporospatial dynamics and association with chromatin-modifying gene expression. J. Neurooncol. 2015-08-14 [PMID: 26272600] (IHC-P, Human)

Bioinformatics

• Gene Symbol: HDAC7
• Entrez:
  • 51564(Human)
• Uniprot:
  • Q8WUI4(Human)