HDAC6 Antibody - BSA Free

Western Blot: HDAC6 Antibody [NB100-56343] - Analysis of HDAC-6 in NIH-3T3 cell lysate with this antibody.

Immunocytochemistry/Immunofluorescence: HDAC6 Antibody [NB100-56343] - HeLa cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-HDAC6 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Simple Western: HDAC6 Antibody [NB100-56343] - Lane view shows a specific band for HDAC6 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, ChIP, ICC/IF, IP, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

HDAC6 Antibody - BSA Free Summary

• Immunogen: Synthetic peptide made to an N-terminal portion of human HDAC6 (between amino acids 1-50) [UniProt Q9UBN7]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HDAC6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 1:20-1:1000
  • Chromatin Immunoprecipitation 1:20-1:1000. Use reported in scientific literature (Imbriano (2005))
  • Immunocytochemistry/ Immunofluorescence 2 - 5 ug/ml
  • Immunoprecipitation assay dependent
  • Simple Western 1:20
  • Western Blot 2 - 5 ug/ml
• Application Notes: In NIH 3T3, a 134 kDa band is observed. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in NIH-3T3 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:10, apparent MW was 131 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Control:

  NIH 3T3 Whole Cell Lysate
  HDAC6 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HDAC6 Antibody - BSA Free

• CPBHM
• EC 3.5.1.98
• FLJ16239
• HD6
• HD6FLJ16239
• HDAC6
• Histone Deacetylase 6
• JM21
• KIAA0901
• PPP1R90

Background

Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins (1). Eight members of HDAC family have been identified in the past several years (2,3). These HDAC family members are divided into two classes, I and II. Class I of the HDAC family comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about twofold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns (3). These results suggested that each member of the HDAC family exhibits a different, individual substrate specificity and function in vivo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HDAC6 Antibody (NB100-56343)(2)

Publications using NB100-56343

• Bandolik JJ, Hamacher A, Schrenk C et al. Class I-Histone Deacetylase (HDAC) Inhibition is Superior to pan-HDAC Inhibition in Modulating Cisplatin Potency in High Grade Serous Ovarian Cancer Cell Lines International Journal of Molecular Sciences 2019-06-22 [PMID: 31234549] (WB, Human)
• Imbriano C, Gurtner A, Cocchiarella F et al. Direct p53 transcriptional repression: in vivo analysis of CCAAT-containing G2/M promoters. Mol Cell Biol. 2005-05-01 [PMID: 15831478]

Bioinformatics

• Gene Symbol: HDAC6
• Entrez:
  • 10013(Human)
  • 15185(Mouse)