HDAC10 Antibody (SR3178) - BSA Free

Western Blot: HDAC10 Antibody (SR3178) [NBP3-27754] - Western blot analysis of HDAC10 expression in (1) HeLa cell lysate; (2) 3T3 cell lysate.

Immunocytochemistry/ Immunofluorescence: HDAC10 Antibody (SR3178) [NBP3-27754] - Immunofluorescent analysis of Hela cells

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IP
• Clone: SR3178
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HDAC10 Antibody (SR3178) - BSA Free Summary

• Description: Novus Biologicals Rabbit HDAC10 Antibody (SR3178) - BSA Free (NBP3-27754) is a recombinant monoclonal antibody validated for use in WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human HDAC10 (Uniprot #: Q969S8)
• Specificity: Detects endogenous levels of HDAC10
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HDAC10
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunoprecipitation 1:50
  • Western Blot 1:1000-1:2000
• Theoretical MW: 75 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 150mM NaCl, 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for HDAC10 Antibody (SR3178) - BSA Free

• DKFZP761B039
• EC 3.5.1.48
• EC 3.5.1.62
• EC 3.5.1.98
• HD10
• HDAC10
• histone deacetylase 10
• MGC149722

Background

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (for p300/CBP-associated factor), p300/CBP and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated mammalian RPD3) and HDAC3, all of which are related to the yeast transcriptional regulator Rpd3p, have been identified as histone deacetylases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HDAC10