HAND1 Antibody (OTI1G10) - Azide and BSA Free

Western Blot: HAND1 Antibody (OTI1G10) - Azide and BSA Free [NBP2-71459] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HAND1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HAND1.

Immunocytochemistry/Immunofluorescence: HAND1 Antibody (OTI1G10) - Azide and BSA Free [NBP2-71459] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY HAND1.

Flow Cytometry: HAND1 Antibody (OTI1G10) - Azide and BSA Free [NBP2-71459] - Analysis of Jurkat cells, using anti-HAND1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Western Blot: HAND1 Antibody (OTI1G10) - Azide and BSA Free [NBP2-71459] - Analysis of extracts (10ug) from 9 Human tissue by using anti-HAND1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).

Flow Cytometry: HAND1 Antibody (OTI1G10) - Azide and BSA Free [NBP2-71459] - Analysis of Hela cells, using anti-HAND1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, CyTOF-ready
• Clone: OTI1G10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

HAND1 Antibody (OTI1G10) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human HAND1 (NP_004812) produced in E.coli.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: HAND1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Western Blot 1:500
• Theoretical MW: 23.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for HAND1 Antibody (OTI1G10) - Azide and BSA Free

• BHLHA27
• bHLHa27heart- and neural crest derivatives-expressed protein 1
• Class A basic helix-loop-helix protein 27
• eHand
• Extraembryonic tissues, heart, autonomic nervous system and neural crestderivatives-expressed protein 1
• HAND1
• heart and neural crest derivatives expressed 1
• Hxt
• Thing1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HAND1