EOMES Antibody

Western blot shows lysates of BG01V human embryonic stem cells untreated (-) or mesoendoderm differentiated (+). PVDF Membrane was probed with 1 µg/mL of Human EOMES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6166) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for EOMES at approximately 85-105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

EOMES was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Human EOMES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6166) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

BG01V human embryonic stem cells differentiated to mesendoderm were stained with Sheep Anti-Human EOMES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6166, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

• Reactivity: Hu
• Applications: WB, CyTOF-ready, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

EOMES Antibody Summary

• Immunogen: E. coli-derived recombinant human EOMES
  Gly471-Pro686
  Accession # O95936
• Specificity: Detects human EOMES in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: EOMES
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EOMES Antibody

• EOMES
• eomesodermin (Xenopus laevis) homolog
• Eomesodermin
• t box, brain, 2
• T-box brain protein 2
• T-box brain2
• TBR2
• TBR-2
• TBR2eomesodermin homolog
• T-brain-2

Background

EOMES (Eomesodermin; Eo from Greek meaning "dawn"/early in mesoderm; also TBR2) is a 72 kDa member of the TBR1 subfamily, T-box family of transcription factors. It is expressed in NK and CD8+ T cells, where CTLA4 activation suppresses EOMES activation of IFN-gamma and granzyme B genes. It is also found in the embryo, where it occurs in forebrain floorplate and migrating neuroblasts at 12.5 weeks gestation. Notably, it is reported to undergo intercellular transfer in fetal Xenopus tissue destined to become mesoderm. Here, it synchronizes a multicellular commitment to a cell lineage. Human EOMES is 686 amino acids (aa) in length. It contains short poly-Ala, -Gly and -Asn motifs, and a DNA-binding T box (aa 276-456). There is one isoform that shows a 19 aa insertion after Ser460. Over aa 471‑686, human EOMES shares 91% aa identity with mouse EOMES.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EOMES Antibody (AF6166)(26)

Publications using AF6166

• Lindenhofer, D;Haendeler, S;Esk, C;Littleboy, JB;Brunet Avalos, C;Naas, J;Pflug, FG;van de Ven, EGP;Reumann, D;Baffet, AD;von Haeseler, A;Knoblich, JA; Cerebral organoids display dynamic clonal growth and tunable tissue replenishment Nature cell biology 2024-05-07 [PMID: 38714853] (Immunohistochemistry, Human)
• Noack, F;Vangelisti, S;Ditzer, N;Chong, F;Albert, M;Bonev, B; Joint epigenome profiling reveals cell-type-specific gene regulatory programmes in human cortical organoids Nature cell biology 2023-11-23 [PMID: 37996647] (IHC, Human)
• Chiaradia, I;Imaz-Rosshandler, I;Nilges, BS;Boulanger, J;Pellegrini, L;Das, R;Kashikar, ND;Lancaster, MA; Tissue morphology influences the temporal program of human brain organoid development Cell stem cell 2023-10-05 [PMID: 37802039] (IHC, Human)
• Perez, Y;Velmeshev, D;Wang, L;White, M;Siebert, C;Baltazar, J;Dutton, NG;Wang, S;Haeussler, M;Chamberlain, S;Kriegstein, A; Single cell analysis of dup15q syndrome reveals developmental and postnatal molecular changes in autism bioRxiv : the preprint server for biology 2023-09-22 [PMID: 37790331] (IHC, Human)
• J Sidhaye, P Trepte, N Sepke, M Novatchkov, M Schutzbier, G Dürnberger, K Mechtler, JA Knoblich Integrated transcriptome and proteome analysis reveals posttranscriptional regulation of ribosomal genes in human brain organoids Elife, 2023-03-29;12(0):. 2023-03-29 [PMID: 36989136] (IHC, Human)
• B Krämer, AP Nalin, F Ma, S Eickhoff, P Lutz, S Leonardell, F Goeser, C Finnemann, G Hack, J Raabe, M ToVinh, S Ahmad, C Hoffmeiste, KM Kaiser, S Manekeller, V Branchi, T Bald, M Hölzel, R Hüneburg, HD Nischalke, A Semaan, B Langhans, DJ Kaczmarek, B Benner, MR Lordo, J Kowalski, A Gerhardt, J Timm, M Toma, R Mohr, A Türler, A Charpentie, T van Bremen, G Feldmann, A Sattler, K Kotsch, AT Abdallah, CP Strassburg, U Spengler, WE Carson, BL Mundy-Boss, M Pellegrini, TE O'Sullivan, AG Freud, J Nattermann Single-cell RNA sequencing identifies a population of human liver-type ILC1s Cell Reports, 2023-01-01;42(1):111937. 2023-01-01 [PMID: 36640314] (Flow Cytometry, IHC, Human)
• P Mesci, JS de Souza, L Martin-San, A Macia, A Saleh, X Yin, C Snethlage, JW Adams, SH Avansini, RH Herai, A Almenar-Qu, Y Pu, RA Szeto, G Goldberg, PT Bruck, F Papes, SK Chanda, AR Muotri SARS-CoV-2 infects human brain organoids causing cell death and loss of synapses that can be rescued by treatment with Sofosbuvir PloS Biology, 2022-11-03;20(11):e3001845. 2022-11-03 [PMID: 36327326] (IHC, Human)
• I Kelava, I Chiaradia, L Pellegrini, AT Kalinka, MA Lancaster Androgens increase excitatory neurogenic potential in human brain organoids Nature, 2022-01-19;0(0):. 2022-01-19 [PMID: 35046577] (IHC, Human)
• Jacopo A. Carpentieri, Amandine Di Cicco, Marusa Lampic, David Andreau, Laurence Del Maestro, Fatima El Marjou et al. Endosomal trafficking defects alter neural progenitor proliferation and cause microcephaly Nature Communications 2022-01-10 [PMID: 35013230]
• G Popova, SS Soliman, CN Kim, MG Keefe, KM Hennick, S Jain, T Li, D Tejera, D Shin, BB Chhun, CS McGinnis, M Speir, ZJ Gartner, SB Mehta, M Haeussler, KB Hengen, RR Ransohoff, X Piao, TJ Nowakowski Human microglia states are conserved across experimental models and regulate neural stem cell responses in chimeric organoids Cell Stem Cell, 2021-09-17;0(0):. 2021-09-17 [PMID: 34536354] (IHC, Human)
• Lin Yang, Zhenmeiyu Li, Guoping Liu, Xiaosu Li, Zhengang Yang Developmental Origins of Human Cortical Oligodendrocytes and Astrocytes Neuroscience Bulletin 2022-01-01 [PMID: 34374948]
• MY Ou, Q Xiao, XC Ju, PM Zeng, J Huang, AL Sheng, ZG Luo The CTNNBIP1-CLSTN1 fusion transcript regulates human neocortical development Cell Reports, 2021-06-29;35(13):109290. 2021-06-29 [PMID: 34192541] (IHC, Human)
• Mirjam Kalusa, Maren D. Heinrich, Christine Sauerland, Markus Morawski, Simone A. Fietz Developmental Differences in Neocortex Neurogenesis and Maturation Between the Altricial Dwarf Rabbit and Precocial Guinea Pig Frontiers in Neuroanatomy 2021-05-31 [PMID: 34135738] (Immunohistochemistry, Guinea Pig, Rabbit)
• Silvia Benito-Kwiecinski, Stefano L. Giandomenico, Magdalena Sutcliffe, Erlend S. Riis, Paula Freire-Pritchett, Iva Kelava et al. An early cell shape transition drives evolutionary expansion of the human forebrain Cell 2021-04-15 [PMID: 33765444]
• Ugomma C. Eze, Aparna Bhaduri, Maximilian Haeussler, Tomasz J. Nowakowski, Arnold R. Kriegstein Single-cell atlas of early human brain development highlights heterogeneity of human neuroepithelial cells and early radial glia Nature Neuroscience 2021-04-01 [PMID: 33723434]
• L Pellegrini, A Albecka, DL Mallery, MJ Kellner, D Paul, AP Carter, LC James, MA Lancaster SARS-CoV-2 Infects the Brain Choroid Plexus and Disrupts the Blood-CSF Barrier in Human Brain Organoids Cell Stem Cell, 2020-10-13;0(0):. 2020-10-13 [PMID: 33113348] (IHC, Human)
• Madeline G Andrews, Lakshmi Subramanian, Arnold R Kriegstein mTOR signaling regulates the morphology and migration of outer radial glia in developing human cortex eLife 2020-09-02 [PMID: 32876565]
• Wei Huang, Aparna Bhaduri, Dmitry Velmeshev, Shaohui Wang, Li Wang, Catherine A. Rottkamp et al. Origins and Proliferative States of Human Oligodendrocyte Precursor Cells Cell 2020-08-01 [PMID: 32679030]
• N Matsumoto, S Tanaka, T Horiike, Y Shinmyo, H Kawasaki A discrete subtype of neural progenitor crucial for cortical folding in the gyrencephalic mammalian brain Elife, 2020-04-21;9(0):. 2020-04-21 [PMID: 32312384] (IHC, Mouse)
• Ayse Güven, Nereo Kalebic, Katherine R Long, Marta Florio, Samir Vaid, Holger Brandl et al. Extracellular matrix-inducing Sox9 promotes both basal progenitor proliferation and gliogenesis in developing neocortex eLife 2020-03-19 [PMID: 32191207]
• Simone A. Fietz, Takashi Namba, Holger Kirsten, Wieland B. Huttner, Robert Lachmann Signs of Reduced Basal Progenitor Levels and Cortical Neurogenesis in Human Fetuses with Open Spina Bifida at 11–15 Weeks of Gestation The Journal of Neuroscience 2020-02-19 [PMID: 31953373]
• Wada H, Ikoma K, Oka Y et al. Human 3D cellular model of hypoxic brain injury of prematurity Nat. Med. 5/1/2019 [PMID: 31061540]
• Pollen AA, Bhaduri A, Andrews MG et al. Establishing Cerebral Organoids as Models of Human-Specific Brain Evolution Cell 2/7/2019 [PMID: 30735633]
• Nicole Barger, Janet Keiter, Anna Kreutz, Anjana Krishnamurthy, Cody Weidenthaler, Verónica Martínez-Cerdeño et al. Microglia: An Intrinsic Component of the Proliferative Zones in the Fetal Rhesus Monkey (Macaca mulatta) Cerebral Cortex Cerebral Cortex 2019-07-05 [PMID: 29992243]
• S Raab, M Klingenste, A Möller, A Illing, J Tosic, M Breunig, G Kuales, L Linta, T Seufferlei, SJ Arnold, A Kleger, S Liebau Reprogramming to pluripotency does not require transition through a primitive streak-like state Sci Rep, 2017-11-29;7(1):16543. 2017-11-29 [PMID: 29185460] (ICC, Western Blot, Human)
• Felipe Mora-Bermúdez, Farhath Badsha, Sabina Kanton, J Gray Camp, Benjamin Vernot, Kathrin Köhler et al. Differences and similarities between human and chimpanzee neural progenitors during cerebral cortex development eLife 2016-09-26 [PMID: 27669147] (Immunohistochemistry, Human, Primate - Pan troglodytes (Chimpanzee), Primate - Pongo (Orangutan))

FAQs for EOMES Antibody (AF6166). (Showing 1 - 1 of 1 FAQs).

1. Please supply a list of eomes antibodies
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Bioinformatics

• Gene Symbol: EOMES
• Uniprot:
  • O95936(Human)