HAND1 Antibody (OTI1G10)

Western Blot: HAND1 Antibody (OTI1G10) [NBP2-00576] - Analysis of extracts (10ug) from 9 Human tissue by using anti-HAND1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).

Immunocytochemistry/Immunofluorescence: HAND1 Antibody (OTI1G10) [NBP2-00576] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY HAND1.

Flow Cytometry: HAND1 Antibody (OTI1G10) [NBP2-00576] - Analysis of Jurkat cells, using anti-HAND1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Western Blot: HAND1 Antibody (OTI1G10) [NBP2-00576] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HAND1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HAND1.

Flow Cytometry: HAND1 Antibody (OTI1G10) [NBP2-00576] - Analysis of Hela cells, using anti-HAND1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, Func, ICC/IF
• Clone: OTI1G10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.82 mg/ml

HAND1 Antibody (OTI1G10) Summary

• Immunogen: Full length human recombinant protein of human HAND1 (NP_004812) produced in E.coli.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: HAND1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • In vitro assay
  • Western Blot 1:500
• Application Notes: Use in In vitro reported in scientific literature (PMID:33316598).
• Theoretical MW: 23.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.82 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HAND1 Antibody (OTI1G10)

• BHLHA27
• bHLHa27heart- and neural crest derivatives-expressed protein 1
• Class A basic helix-loop-helix protein 27
• eHand
• Extraembryonic tissues, heart, autonomic nervous system and neural crestderivatives-expressed protein 1
• HAND1
• heart and neural crest derivatives expressed 1
• Hxt
• Thing1

Background

The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, it has been suggested that this transcription factor may be required for early trophoblast differentiation. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HAND1 Antibody (NBP2-00576)(1)

Publication using NBP2-00576

• Leavens KF, Liao CM, Gagne AL et al. Generation of a double insulin and somatostatin reporter line, SCSe001-A-3, for the advancement of stem cell-derived pancreatic islets Stem cell research 2020-12-08 [PMID: 33316598] (In vitro, Human)

Bioinformatics

• Gene Symbol: HAND1
• Entrez:
  • 9421(Human)