HAND1 Antibody (OTI1G10)

Images

 
Western Blot: HAND1 Antibody (OTI1G10) [NBP2-00576] - Analysis of extracts (10ug) from 9 Human tissue by using anti-HAND1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; ...read more
Immunocytochemistry/ Immunofluorescence: HAND1 Antibody (OTI1G10) [NBP2-00576] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY HAND1.
Flow Cytometry: HAND1 Antibody (OTI1G10) [NBP2-00576] - Analysis of Jurkat cells, using anti-HAND1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: HAND1 Antibody (OTI1G10) [NBP2-00576] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HAND1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Flow Cytometry: HAND1 Antibody (OTI1G10) [NBP2-00576] - Analysis of Hela cells, using anti-HAND1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, In vitro
Clone
OTI1G10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.82 mg/ml

Order Details

HAND1 Antibody (OTI1G10) Summary

Immunogen
Full length human recombinant protein of human HAND1 (NP_004812) produced in E.coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
HAND1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • In vitro assay
Application Notes
Use in In vitro reported in scientific literature (PMID:33316598).
Theoretical MW
23.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-00576 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.82 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HAND1 Antibody (OTI1G10)

  • BHLHA27
  • bHLHa27heart- and neural crest derivatives-expressed protein 1
  • Class A basic helix-loop-helix protein 27
  • eHand
  • Extraembryonic tissues, heart, autonomic nervous system and neural crestderivatives-expressed protein 1
  • HAND1
  • heart and neural crest derivatives expressed 1
  • Hxt
  • Thing1

Background

The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, it has been suggested that this transcription factor may be required for early trophoblast differentiation. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HAND1 Antibody (NBP2-00576)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: In vitro.


Filter By Application
In vitro
(1)
All Applications
Filter By Species
Human
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HAND1 Antibody (NBP2-00576) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-71459AF350
Alexa Fluor 405 NBP2-71459AF405
Alexa Fluor 488 NBP2-71459AF488
Alexa Fluor 532 NBP2-71459AF532
Alexa Fluor 594 NBP2-71459AF594
Alexa Fluor 647 NBP2-71459AF647
Alexa Fluor 700 NBP2-71459AF700
Alexa Fluor 750 NBP2-71459AF750
Allophycocyanin NBP2-71459APC
Allophycocyanin/Cy7 NBP2-71459APCCY7
Biotin NBP2-71459B
DyLight 350 NBP2-71459UV
DyLight 405 NBP2-71459V
DyLight 488 NBP2-71459G
DyLight 550 NBP2-71459R
DyLight 594 NBP2-71459DL594
DyLight 650 NBP2-71459C
DyLight 680 NBP2-71459FR
DyLight 755 NBP2-71459IR
FITC NBP2-71459F
HRP NBP2-71459H
Janelia Fluor 549 NBP2-71459JF549
Janelia Fluor 646 NBP2-71459JF646
PE NBP2-71459PE
PE/Atto594 NBP2-71459PEATT594
PE/Cy5.5 NBP2-71459PECY55
PE/Cy7 NBP2-71459PECY7
PerCP NBP2-71459PCP

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Bioinformatics

Gene Symbol HAND1
Entrez