GRP78/HSPA5 Antibody [Biotin]

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Product Details

Summary
Reactivity Hu, Mu, Rt, SyHa, PmSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Biotin

GRP78/HSPA5 Antibody [Biotin] Summary

Immunogen
Synthetic peptide made to a C-terminal portion of rat GRP78 (within residues 600-654). [Swiss-Prot# P06761]
Localization
Endoplasmic reticulum lumen. Melanosome. Note= Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Marker
ER Stress Marker
Predicted Species
Primate (92%), Golden Syrian Hamster (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HSPA5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
Application Notes
This GRP78 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at ~78 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Readout System

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for GRP78/HSPA5 Antibody [Biotin]

  • 78 kDa glucose-regulated protein
  • BIP
  • Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78
  • FLJ26106
  • GRP78
  • GRP-78
  • GRP78BIP
  • Heat shock 70 kDa protein 5
  • heat shock 70kD protein 5 (glucose-regulated protein, 78kD)
  • heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
  • HSP70-5
  • HSPA5
  • Immunoglobulin heavy chain-binding protein
  • MIF2

Background

GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell. BiP is also overexpressed in many cancers and has been hypothesized to actively regulate multiple malignant phenotypes including cell growth, migration, and invasion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GRP78/HSPA5 Antibody (NBP1-06277B) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 488 Labeled NBP1-06277G
DyLight 550 Labeled NBP1-06277R
DyLight 650 Labeled NBP1-06277C
HRP Labeled NBP1-06277H

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Bioinformatics Tool for GRP78/HSPA5 Antibody (NBP1-06277B)

Discover related pathways, diseases and genes to GRP78/HSPA5 Antibody (NBP1-06277B). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on GRP78/HSPA5.

GRP78 - molecular chaperone and negative regulator of the unfolded protein response
The 78 kDa glucose-regulated protein (GRP78) is the eukaryotic orthologue to the prokaryotic heat shock 70 kDa protein 5 (HSPA5). GRP78 is also sometimes referred to as BiP. GRP78 is a member of the HSP70 family and plays dynamic roles in protein...  Read full blog post.

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Bioinformatics

Gene Symbol HSPA5
Entrez