Granulin Antibody (CL5692) - BSA Free

Immunohistochemistry-Paraffin: Granulin Antibody (CL5692) [NBP2-61424] - Staining of human skeletal muscle shows absence of positivity in striated muscle fibers as expected (negative control).

Immunohistochemistry-Paraffin: Granulin Antibody (CL5692) [NBP2-61424] - Staining of human prostate shows moderate granular cytoplasmic immunoreactivity in glandular cells.

Immunohistochemistry-Paraffin: Granulin Antibody (CL5692) [NBP2-61424] - Staining of human kidney shows cytoplasmic immunoreactivity in both renal glomeruli and tubules.

Immunohistochemistry-Paraffin: Granulin Antibody (CL5692) [NBP2-61424] - Staining of human small intestine shows moderate granular cytoplasmic positivity in glandular cells.

• Reactivity: Hu
• Applications: IHC
• Clone: CL5692
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Granulin Antibody (CL5692) - BSA Free Summary

• Description: Novus Biologicals Mouse Granulin Antibody (CL5692) - BSA Free (NBP2-61424) is a monoclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a recombinant protein derived from P28799, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GRN
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  Granulin Recombinant Protein Antigen (NBP2-61424PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for Granulin Antibody (CL5692) - BSA Free

• acrogranin
• GEP
• GP88
• granulin
• granulin-epithelin
• granulins
• PC cell-derived growth factor
• PCDGF
• PEPI
• PGRN
• proepithelin
• progranulin

Background

Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GRN