GR/NR3C1 Antibody - Azide and BSA Free

Images

 
Immunocytochemistry/ Immunofluorescence: GR/NR3C1 Antibody (59) [NB120-3579] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Polyclonal Antibody (59) shows staining in Hela Cells. Glucocorticoid ...read more
Immunocytochemistry/ Immunofluorescence: GR/NR3C1 Antibody (59) [NB120-3579] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Polyclonal Antibody (59) shows staining in A2058 Cells. Glucocorticoid ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, Simple Western, ChIP, GS, ICC/IF, IHC, IHC-Fr, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

GR/NR3C1 Antibody - Azide and BSA Free Summary

Immunogen
Synthetic peptide corresponding to residues C(245) K P L I L P D T K P K I K D(259) of human GR.
Localization
Cytoplasmic in the absence of ligand; nuclear after ligand-binding.
Specificity
NB120-3579 detects glucocorticoid receptor (GR) from human, mouse and rat tissues.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NR3C1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Gel Super Shift Assays 1:1 - 1:100
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1 ug/ml
  • Immunohistochemistry-Frozen 1 ug/ml
  • Immunoprecipitation 5 ug/ml
  • Simple Western
  • Western Blot 5 ug/ml
Application Notes
WB: Detects an approx. 97 kDa protein representing GR as well as two other unidentified proteins at approx. 135 - 145 kDa from rat liver extract. IHC: Staining of GR in rat hippocampus using NB120-3579 results in staining primarily in the nucleus. NB120-3579 does not disrupt receptor-DNA interactions.
Simple Western reported by an internal validation. Separated by Size- Wes/Sally Sue/Peggy Sue, antibody dilution of 1:10. Apparent MW in kDa on Simple Western was 48, 61, 95kDa; matrix was 12-230 kDa.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS.

Alternate Names for GR/NR3C1 Antibody - Azide and BSA Free

  • GCR
  • glucocorticoid receptor
  • GR
  • GRGCCR
  • GRLNuclear receptor subfamily 3 group C member 1
  • NR3C1
  • nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
  • nuclear receptor subfamily 3, group C, member 1

Background

Steroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocoticoid receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain glucocorticoid receptor. The corresponding gene for the glucocorticoid receptor is NR3C1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF3398
Species: Hu, Mu, Rt
Applications: ICC, KO, Simple Western, WB
NBP2-76932
Species: Hu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NB100-1533
Species: Hu, Mu, Po, Rt, Sh
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA, WB
NB300-562
Species: Ch, Hu, Mu, Rb, Rt, Sh
Applications: B/N, Flow, ICC/IF, IHC, IHC-P, WB
NBP2-24915
Species: Bv, Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP1-86615
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-58917
Species: Hu
Applications: IHC, IHC-P, WB
7570-GH
Species: Hu
Applications: EnzAct
NBP2-67471
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
AF5415
Species: Hu
Applications: ICC, IHC, Simple Western, WB
210-TA/CF
Species: Hu
Applications: BA
NB100-56603
Species: Ca, Hu, Mu, Pm, Rt
Applications: IHC, IHC-P, WB
NBP2-80461
Species: Hu
Applications: Flow, IHC, IHC-P, WB
NBP2-49118
Species: Hu
Applications: IHC, IHC-P
NBP1-89065
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
H00001392-M02
Species: Hu
Applications: ELISA, IHC, IHC-P, S-ELISA, WB
D6050
Species: Hu
Applications: ELISA
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow

Publications for GR/NR3C1 Antibody (NB120-3579) (0)

There are no publications for GR/NR3C1 Antibody (NB120-3579).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for GR/NR3C1 Antibody (NB120-3579) (0)

There are no reviews for GR/NR3C1 Antibody (NB120-3579). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for GR/NR3C1 Antibody (NB120-3579) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional GR/NR3C1 Products

Bioinformatics Tool for GR/NR3C1 Antibody (NB120-3579)

Discover related pathways, diseases and genes to GR/NR3C1 Antibody (NB120-3579). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for GR/NR3C1 Antibody (NB120-3579)

Discover more about diseases related to GR/NR3C1 Antibody (NB120-3579).
 

Pathways for GR/NR3C1 Antibody (NB120-3579)

View related products by pathway.

PTMs for GR/NR3C1 Antibody (NB120-3579)

Learn more about PTMs related to GR/NR3C1 Antibody (NB120-3579).
 

Research Areas for GR/NR3C1 Antibody (NB120-3579)

Find related products by research area.

Blogs on GR/NR3C1.

Epigenetics of Depression: How Can Psychological Stress Alter Your DNA?
By Emily Cartwright, PhDHow Can Psychological Stress Alter Your DNA? Traumatic events, work demands, relationship conflicts, and health problems are all examples of psychological stressors that can result in phy...  Read full blog post.

Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our GR/NR3C1 Antibody - Azide and BSA Free and receive a gift card or discount.

Bioinformatics

Gene Symbol NR3C1
Entrez
Uniprot