CSRP3 Antibody

Western Blot: CSRP3 Antibody [NBP1-06983] - (0.01ug/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Immunohistochemistry-Paraffin: CSRP3 Antibody [NBP1-06983] - staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: CSRP3 Antibody [NBP1-06983] - Staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

CSRP3 Antibody Summary

• Description: Novus Biologicals Goat CSRP3 Antibody (NBP1-06983) is a polyclonal antibody validated for use in IHC, WB, ELISA, ICC/IF and IP. Anti-CSRP3 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence CGKSLESTNVTDKD corresponding to internal region according to NP_003467.1.
• Epitope: internal region
• Predicted Species: Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CSRP3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Immunoprecipitation
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.01-0.03 ug/ml
• Application Notes: Western blot: Approx. 20kDa band observed in Human, Mouse and Rat Heart lysates (calculated MW of 21.0kDa according to Human NP_003467.1 and 20.8kDa according to Rat NP_476485.1). Primary incubation was 1 hour.
  IHC: Paraffin embedded Human Skeletal Muscle and Heart.
  Use in Immunocytochemistry/immunofluorescence and Immunoprecipitation reported in scientific literature PMID: 27551448.
• Control:

  CSRP3 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CSRP3 Antibody

• Cardiac LIM protein
• CLPMGC14488
• CMD1Mcardiac LIM domain protein
• CMH12
• CRP3cysteine and glycine-rich protein 3
• cysteine and glycine-rich protein 3 (cardiac LIM protein)
• Cysteine-rich protein 3
• LIM domain only 4
• LIM domain protein, cardiac
• LMO4
• MLPMGC61993
• Muscle LIM protein

Background

This gene encodes a member of the CSRP family of LIM domain proteins, which may be involved in regulatory processes important for development and cellular differentiation. The LIM/double zinc-finger motif found in this protein is found in a group of proteins with critical functions in gene regulation, cell growth, and somatic differentiation. Mutations in this gene are thought to cause heritable forms of hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM) in humans. Alternatively spliced transcript variants with different 5' UTR, but encoding the same protein, have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CSRP3 Antibody (NBP1-06983)(2)

Publications using NBP1-06983

• Rashid MM, Runci A, Polletta L et al. Muscle LIM protein/CSRP3: a mechanosensor with a role in autophagy. Cell Death Discov. [PMID: 27551448] (IP, ICC/IF, WB, Mouse)
• Zheng B, Wen JK, Han M. hhLIM is a novel F-actin binding protein involved in actin cytoskeleton remodeling. FEBS J 2008-04-01 [PMID: 18331358]

Bioinformatics

• Gene Symbol: CSRP3
• Entrez:
  • 8048(Human)
  • 13009(Mouse)
  • 117505(Rat)
• Uniprot:
  • NP_003467.1(Human)