CSRP3 Antibody

Images

 
Western Blot: CSRP3 Antibody [NBP1-06983] - (0.01ug/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry-Paraffin: CSRP3 Antibody [NBP1-06983] - staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: CSRP3 Antibody [NBP1-06983] - Staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC-P, IP, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

CSRP3 Antibody Summary

Immunogen
Peptide with sequence CGKSLESTNVTDKD corresponding to internal region according to NP_003467.1.
Epitope
internal region
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CSRP3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.01-0.03 ug/ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Immunoprecipitation
  • Peptide ELISA Detection limit 1:32000
Application Notes
Western blot: Approx. 20kDa band observed in Human, Mouse and Rat Heart lysates (calculated MW of 21.0kDa according to Human NP_003467.1 and 20.8kDa according to Rat NP_476485.1). Primary incubation was 1 hour.
IHC: Paraffin embedded Human Skeletal Muscle and Heart.
Use in Immunocytochemistry/immunofluorescence and Immunoprecipitation reported in scientific literature PMID: 27551448.
Control
CSRP3 Overexpression Lysate
Publications
Read Publications using
NBP1-06983 in the following applications:

  • 1 publication
  • IP
    1 publication
  • WB
    1 publication

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CSRP3 Antibody

  • Cardiac LIM protein
  • CLPMGC14488
  • CMD1Mcardiac LIM domain protein
  • CMH12
  • CRP3cysteine and glycine-rich protein 3
  • cysteine and glycine-rich protein 3 (cardiac LIM protein)
  • Cysteine-rich protein 3
  • LIM domain only 4
  • LIM domain protein, cardiac
  • LMO4
  • MLPMGC61993
  • Muscle LIM protein

Background

This gene encodes a member of the CSRP family of LIM domain proteins, which may be involved in regulatory processes important for development and cellular differentiation. The LIM/double zinc-finger motif found in this protein is found in a group of proteins with critical functions in gene regulation, cell growth, and somatic differentiation. Mutations in this gene are thought to cause heritable forms of hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM) in humans. Alternatively spliced transcript variants with different 5' UTR, but encoding the same protein, have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CSRP3 Antibody (NBP1-06983)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: ICC/IF, IP, WB.


Filter By Application
ICC/IF
(1)
IP
(1)
WB
(1)
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Mouse
(1)
All Species

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CSRP3
Entrez
Uniprot