gp96/HSP90B1/GRP94 Antibody - BSA Free

Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP90 beta antibody diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody

Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - GRP94 Antibody [NBP1-32118] - Analysis of methanol-fixed HCT116, using antibody at 1:500 dilution.

Immunohistochemistry: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - Mouse lung. HSP90 beta antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - GRP94 Antibody [NBP1-32118] - Sample(30 ug of whole cell lysate)A:293TB:A431 7. 5% SDS PAGE, antibody diluted at 1:1500.

Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP90 beta antibody diluted at 1:1500. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - GRP94 Antibody [NBP1-32118] - Paraffin-embedded BT483 xenograft, using antibody at 1:100 dilution.

Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - Mouse skin. HSP90 beta antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

IHC-WM-gp96/HSP90B1/GRP94 Antibody [NBP1-32118] -Hsp90 beta antibody detects Hsp90 beta protein on whole-mount zebrafish embryos by immunohistochemical analysis.Sample: Paraformaldehyde-fixed zebrafish embryos.Hsp90 beta antibody dilution: 1:200.

Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - Sample (30 ug of whole cell lysate) A: zebrafish eye 7.5% SDS PAGE NBP1-32118 diluted at 1:10000

• Reactivity: Hu, Mu, Rt, Ze
• Applications: WB, ELISA, ICC/IF, IHC, IP, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

gp96/HSP90B1/GRP94 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit gp96/HSP90B1/GRP94 Antibody - BSA Free (NBP1-32118) is a polyclonal antibody validated for use in IHC, WB, ELISA, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human gp96/HSP90B1/GRP94. The exact sequence is proprietary.
• Localization: Cytoplasm , Melanosome
• Marker: ER Stress Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HSP90B1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Assay dependent
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry Whole-Mount Assay dependent
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation Assay dependent
  • Western Blot 1:500-1:3000
• Theoretical MW: 83 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for gp96/HSP90B1/GRP94 Antibody - BSA Free

• 94 kDa glucose-regulated protein
• ECGP
• Endoplasmin
• endothelial cell (HBMEC) glycoprotein
• glucose regulated protein, 94 kDa
• gp96
• GP96ECGP
• Grp94
• GRP-94
• GRP94gp96 homolog
• Heat shock protein 90 kDa beta member 1
• heat shock protein 90kDa beta (Grp94), member 1
• HSP90B1
• TRA1endoplasmin
• tumor rejection antigen (gp96) 1
• Tumor rejection antigen 1
• Tumor rejection antigen-1 (gp96)

Background

Hsp90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. From a functional perspective, hsp90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex (1-4). Despite its label of being a heat-shock protein, hsp90 is one of the most highly expressed proteins in unstressed cells (1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: HSP90B1