GOLPH3 Antibody

Images

 
Western Blot: GOLPH3 Antibody [NBP1-76241] - Western blot analysis of GOLPH3 in rat lung tissue lysate with GOLPH3 antibody at (A) 0.5 and (B) 1 ug/mL.
Immunocytochemistry/ Immunofluorescence: GOLPH3 Antibody [NBP1-76241] - Immunofluorescence of GOLPH3 in Rat Lung cells with GOLPH3 antibody at 20 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

GOLPH3 Antibody Summary

Immunogen
Antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human GOLPH3. The immunogen is located within the first 50 amino acids of GOLPH3.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GOLPH3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
Control Peptide
GOLPH3 Peptide (NBP1-76241PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GOLPH3 Antibody

  • Coat protein GPP34
  • FLJ90675
  • golgi phosphoprotein 3 (coat-protein)
  • Golgi phosphoprotein 3
  • golgi protein
  • golgi-associated protein
  • GOPP1
  • GPP34coat-protein
  • MIDASgolgi peripheral membrane protein 1, 34 kDa
  • Mitochondrial DNA absence factor

Background

GOLPH3 was initially identified as a peripheral membrane protein localized to the trans-Golgi network, but others reported it to be primarily a mitochondrial protein that regulated the mitochondrial mass through the regulation of the mitochondria-specific phospholipid cardiolipin. GOLPH3 has since been implicated in the target of rapamycin (TOR) signalling pathway. Its overexpression in transfected cells led to and increase in anchorage-independent growth and cell proliferation in vitro. Furthermore, GOLPH3-transfected cells enhanced S6 Kinase activity in response to growth factor stimulation by EGF. Simultaneously, AKT phosphorylation increased in these cells, while these events were abrogated in GOLPH3 siRNA treated cells compared to control cells, indicating the GOLPH3 can enhance signalling through TOR-associated complexes. These results suggest that GOLPH3 is a bona fide oncogene and may be a useful target for therapeutic strategies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GOLPH3
Entrez
Uniprot