GM130/GOLGA2 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [NBP2-53420] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NBP2-53420 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [NBP2-53420] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-GM130/GOLGA2 Antibody NBP2-53420 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [NBP2-53420] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-GM130/GOLGA2 Antibody NBP2-53420 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [NBP2-53420] - HeLa cells were fixed for 10 min using 10% formalin and then permeabilized for 5 min using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-GOLGA2 at 1:200 overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at 1:500. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at 1:1000 and detected with an anti-mouse DyLight 550 (Red) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Western Blot: GM130/GOLGA2 Antibody [NBP2-53420] - Total protein from human HeLa and mouse Neuro2A cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/mL anti-GOLGA2 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP2-53420] - Analysis of a FFPE tissue section of mouse intesting using GM130/GOLGA2 antibody at 1:1500. The antibody generated staining in the the Golgi apparatus in the perinuclear region.

Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [NBP2-53420] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with GM130/GOLGA2 Antibody conjugated to Biotin (NBP2-53420B) at 5 ug/ml for 1 hour at room temperature and detected with Streptavidin conjugated to DyLight 488. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [NBP2-53420] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with GM130/GOLGA2 Antibody conjugated to FITC (NBP2-53420F) at 2 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Flow Cytometry: GM130/GOLGA2 Antibody [NBP2-53420] - An intracellular stain was performed on NIH3T3 cells with NBP2-53420 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [NBP2-53420] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with GM130/GOLGA2 Antibody conjugated to FITC (NBP2-53420F) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Flow Cytometry: GM130/GOLGA2 Antibody [NBP2-53420] - An intracellular stain was performed on HeLa cells with NBP2-53420AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 min at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Flow Cytometry: GM130/GOLGA2 Antibody [NBP2-53420] - An intracellular stain was performed on MCF7 cells with GM130/GOLGA2 Antibody NBP2-53420AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Flow Cytometry: GM130/GOLGA2 Antibody [NBP2-53420] - An intracellular stain was performed on HeLa cells with NBP2-53420 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

GM130/GOLGA2 Antibody - BSA Free Summary

• Immunogen: Partial recombinant human GM130/GOLGA2 protein (amino acids 528-606). [UniPro Q08379]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GOLGA2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1-5 ug/mL
  • Immunohistochemistry 1:1000 - 1:1500
  • Immunohistochemistry-Paraffin 1:1000 - 1:1500
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GM130/GOLGA2 Antibody - BSA Free

• 130 kDa cis-Golgi matrix protein
• GM130 autoantigen
• GM130
• GM130Golgi matrix protein GM130
• GOLGA2
• golgi autoantigen, golgin subfamily a, 2
• golgin A2
• Golgin subfamily A member 2
• Golgin-95
• MGC20672
• SY11 Protein

Background

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. GM130 encodes a member of the Golgin protein family and is localised to the Golgi. The protein encoded by GM130 has been postulated to play roles in the stacking of Golgi cisternae, maintaining cis-Golgi structure and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. GM130 is part of a larger oligomeric complex which interacts with p115, GORASP1/GRASP65 and RAB1B that has been activated by GTP-binding. GM130 is useful as a cis-Golgi marker in immunohistochemistry.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GM130/GOLGA2 Antibody (NBP2-53420)(10)

Publications using NBP2-53420

• Brodsky IB, Fokin AI, Efremov AA et al. Divergent Contribution of the Golgi Apparatus to Microtubule Organization in Related Cell Lines International journal of molecular sciences 2022-12-19 [PMID: 36555819] (ICC/IF, Monkey)
• Kuhn M, Zhang Y, Favate J et al. IMP1/IGF2BP1 in human colorectal cancer extracellular vesicles American journal of physiology. Gastrointestinal and liver physiology 2022-10-04 [PMID: 36194131] (WB, Human)
  
  Details:
  Dilution used in WB 1:1000
• Jacquier A, Risson V, Simonet T et al. Severe congenital myasthenic syndromes caused by agrin mutations affecting secretion by motoneurons Acta neuropathologica 2022-08-10 [PMID: 35948834] (ICC/IF)
  
  Details:
  Dilutions: 1:400
• Vijayan K, Arang N, Wei L et al. A genome-wide CRISPR-Cas9 screen identifies CENPJ as a host regulator of altered microtubule organization during Plasmodium liver infection Cell chemical biology 2022-06-18 [PMID: 35738280] (ICC/IF)
• Zhang X, Wang X, Yuan Z et al. Amino acids-Rab1A-mTORC1 signaling controls whole-body glucose homeostasis Cell reports 2021-03-16 [PMID: 33730578]
• Shomron O, Hirschberg K, Burakov A et al. Positioning of endoplasmic reticulum exit sites around the Golgi depends on BicaudalD2 and Rab6 activity Traffic (Copenhagen, Denmark) 2020-12-12 [PMID: 33314495]
• Li F, Xu R, Lin CL et al. Maternal and fetal alkaline ceramidase 2 is required for placental vascular integrity in mice FASEB J. 2020-09-22 [PMID: 32959379] (WB, Mouse)
• Park SS, Ponce-Balbuena D, Kuick R et al. Kir2.1 interactome mapping uncovers PKP4 as a modulator of the Kir2.1-regulated inward rectifier potassium currents Mol. Cell Proteomics 2020-06-15 [PMID: 32541000]
• Kuang L, Hashimoto K, Huang EJ et al. Frontotemporal dementia nonsense mutation of progranulin rescued by aminoglycosides Hum. Mol. Genet. 2020-01-08 [PMID: 31913476] (ICC/IF, WB, Mouse)
• Ghoshal, A;Rodrigues, LC;Gowda, CP;Elcheva, IA;Liu, Z;Abraham, T;Spiegelman, VS; Extracellular vesicle-dependent effect of RNA-binding protein IGF2BP1 on melanoma metastasis Oncogene 2019-04-01 [PMID: 30936459] (WB, Mouse)

Bioinformatics

• Gene Symbol: GOLGA2