GM130/GOLGA2 Antibody

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Immunocytochemistry/ Immunofluorescence: GM130/GOLGA2 Antibody [NBP2-53420] - HeLa cells were fixed for 10 min using 10% formalin and then permeabilized for 5 min using 1X TBS + 0.5% Triton X-100. The cells were ...read more
Western Blot: GM130/GOLGA2 Antibody [NBP2-53420] - Total protein from human HeLa and mouse Neuro2A cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The ...read more
Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP2-53420] - Analysis of a FFPE tissue section of mouse intesting using GM130/GOLGA2 antibody at 1:1500. The antibody generated staining in the the Golgi apparatus ...read more
Flow Cytometry: GM130/GOLGA2 Antibody [NBP2-53420] - An intracellular stain was performed on MCF7 cells with GM130/GOLGA2 Antibody NBP2-53420AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% ...read more
Flow Cytometry: GM130/GOLGA2 Antibody [NBP2-53420] - An intracellular stain was performed on HeLa cells with NBP2-53420AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

GM130/GOLGA2 Antibody Summary

Immunogen
Partial recombinant human GM130/GOLGA2 protein (amino acids 528-606). [UniPro Q08379]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GOLGA2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 5 ug/mL
  • Immunohistochemistry 1:1000 - 1:1500
  • Immunohistochemistry-Paraffin 1:1000 - 1:1500
Control
GM130/GOLGA2 Knockout HeLa Cell Lysate
Publications
Read Publications using
NBP2-53420 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GM130/GOLGA2 Antibody

  • 130 kDa cis-Golgi matrix protein
  • GM130 autoantigen
  • GM130
  • GM130Golgi matrix protein GM130
  • GOLGA2
  • golgi autoantigen, golgin subfamily a, 2
  • golgin A2
  • Golgin subfamily A member 2
  • Golgin-95
  • MGC20672
  • SY11 Protein

Background

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. GM130 encodes a member of the Golgin protein family and is localised to the Golgi. The protein encoded by GM130 has been postulated to play roles in the stacking of Golgi cisternae, maintaining cis-Golgi structure and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. GM130 is part of a larger oligomeric complex which interacts with p115, GORASP1/GRASP65 and RAB1B that has been activated by GTP-binding. GM130 is useful as a cis-Golgi marker in immunohistochemistry.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GM130/GOLGA2 Antibody (NBP2-53420)(3)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GM130/GOLGA2 Antibody (NBP2-53420) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-53420AF350
Alexa Fluor 405 NBP2-53420AF405
Alexa Fluor 488 NBP2-53420AF488
Alexa Fluor 532 NBP2-53420AF532
Alexa Fluor 594 NBP2-53420AF594
Alexa Fluor 647 NBP2-53420AF647
Alexa Fluor 700 NBP2-53420AF700
Alexa Fluor 750 NBP2-53420AF750
Biotin NBP2-53420B
DyLight 350 NBP2-53420UV
DyLight 405 NBP2-53420V
DyLight 488 NBP2-53420G
DyLight 550 NBP2-53420R
DyLight 594 NBP2-53420DL594
DyLight 650 NBP2-53420C
DyLight 680 NBP2-53420FR
DyLight 755 NBP2-53420IR
FITC NBP2-53420F
HRP NBP2-53420H
Janelia Fluor 549 NBP2-53420JF549
Janelia Fluor 646 NBP2-53420JF646

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Blogs on GM130/GOLGA2

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Bioinformatics

Gene Symbol GOLGA2