GOLM1 Antibody

Images

 
Western Blot: GOLM1 Antibody [NBP1-50627] - Western Blot Image of GOLM1. Whole cell protein from HeLa (lane 1), 3T3 (lane 2), MEF (lane 3), Raw264.7 (lane 4), L929 (lane 5), Neuro2A (lane 6) and Hek293 (lane 7) were ...read more
Immunocytochemistry/ Immunofluorescence: GOLM1 Antibody [NBP1-50627] - Confocal immunofluorescent analysis of HeLa cells using GOLM1 antibody (NBP1-50627, 1:25). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used ...read more
Immunocytochemistry/ Immunofluorescence: GOLM1 Antibody [NBP1-50627] - ICC staining of GOLM1 in HEK293T cells with FITC (green). Nuclei (blue) were counterstained with Dapi.
Immunocytochemistry/ Immunofluorescence: GOLM1 Antibody [NBP1-50627] - GOLM1 antibody was tested in HEK-293 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.61 mg/ml

Order Details

GOLM1 Antibody Summary

Immunogen
A genomic peptide made to an internal region of the human GOLM1 protein (within residues 250-400). [Swiss-Prot Q8NBJ4]
Localization
Golgi apparatus - cis-Golgi network membrane
Marker
Golgi Apparatus Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GOLM1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:5000
  • Immunocytochemistry/Immunofluorescence 1:500
Application Notes
This GOLPH2 antibody is useful for ICC/IF and Western blot.
Positive Control
HeLa Lysate (NB800-PC1)
Reviewed Applications
Read 2 Reviews rated 5
using
NBP1-50627 in the following applications:

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 0.1% BSA, and 50% Glycerol
Preservative
0.05% Sodium Azide
Concentration
0.61 mg/ml
Purity
Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for GOLM1 Antibody

  • bA379P1.3
  • FLJ23608
  • golgi membrane protein 1
  • Golgi membrane protein GP73
  • Golgi phosphoprotein 2C9orf155
  • golgi protein, 73-kD
  • GOLPH2 FLJ22634
  • GP73 chromosome 9 open reading frame 155
  • PSEC0257

Background

In liver, prostate and lung, both viral and non-viral related disease is correlated to the upregulation of GOLPH2, also known as GOLM1, GP73 or Golgi phosphoprotein 2. While it remains unclear how viral insertions are manifested into the Golgi apparatus and subsequent shepherding of proteins, GOLPH2 seems to be affected by the insertions in such a way that it can be attributed to disease phenotypes. Furthermore, because GOLPH2 is so uniquely regulated in cells, it can be successfully used as a clinically relevant molecular biomarker for cancer. As it is secreted and excreted, GOLPH2 is a useful biomarker because of relative ease of sampling in urine and direct correlation to urine concentrations and disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GOLM1 Antibody (NBP1-50627) (0)

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Reviews for GOLM1 Antibody (NBP1-50627) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.
We have 2 reviews tested in 2 application: ICC, WB.

Reviews using NBP1-50627:
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ICC
(1)
WB
(1)
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Human
(2)
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Images Ratings Applications Species Date Details
Immunocytochemistry GOLM1 NBP1-50627
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reviewed by:
Anony- mous
ICC Human 06/12/2014
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Summary

ApplicationImmunocytochemistry
SpeciesHuman
Special ApplicationsConfocal immunofluorescent analysis of HeLa cells using GOLM1 antibody (NBP1-50627, 1:25). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
FileView PDF
Western Blot GOLM1 NBP1-50627
Enlarge
reviewed by:
Anony- mous
WB Human 06/12/2014
View

Summary

ApplicationWestern Blot
SpeciesHuman
Special ApplicationsWestern blot analysis of extracts from LNCaP cells using GOLM1 antibody (NBP1-50627, 1:5000).
FileView PDF

Product General Protocols

View specific protocols for GOLM1 Antibody (NBP1-50627): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GOLM1 Antibody (NBP1-50627) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for GOLM1 Antibody (NBP1-50627)

Discover related pathways, diseases and genes to GOLM1 Antibody (NBP1-50627). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on GOLM1.

6 Fictional Character Protein Names
Check out our list of some of the funniest fictional character protein names including GOLM1, SMURF1, HOMER1, Hunchback, Lilliputian, Pokemon, Tinman Paralog, and Draculin. What are your favorite or funniest protein names? Share them in the comments;...  Read full blog post.

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Recent Reviews

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2
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1
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Anony- mous
06/12/2014
Application: ICC
Species: Human

Anony- mous
06/12/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol GOLM1
Entrez
Uniprot