GOLM1 Antibody

Images

 
Genetic Strategies: Knockout Validated: GOLM1 Antibody [NBP1-50627] - Western blot shows lysates of HEK293 human embryonic kidney parental cell line and GOLM1 knockout (KO) HEK293 human embryonic kidney cell ...read more
Western Blot: GOLM1 Antibody [NBP1-50627] - Western Blot Image of GOLM1. Whole cell protein from HeLa (lane 1), 3T3 (lane 2), MEF (lane 3), Raw264.7 (lane 4), L929 (lane 5), Neuro2A (lane 6) and PC12 (lane 7) were ...read more
Immunocytochemistry/ Immunofluorescence: GOLM1 Antibody [NBP1-50627] - Confocal immunofluorescent analysis of HeLa cells using GOLM1 antibody (NBP1-50627, 1:25). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used ...read more
Immunocytochemistry/ Immunofluorescence: GOLM1 Antibody [NBP1-50627] - ICC staining of GOLM1 in HEK293T cells with FITC (green). Nuclei (blue) were counterstained with Dapi.
Immunocytochemistry/ Immunofluorescence: GOLM1 Antibody [NBP1-50627] - GOLM1 antibody was tested in HEK-293 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.61 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

GOLM1 Antibody Summary

Immunogen
A genomic peptide made to an internal region of the human GOLM1 protein (within residues 250-400). [Swiss-Prot Q8NBJ4]
Localization
Golgi apparatus - cis-Golgi network membrane
Marker
Golgi Apparatus Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GOLM1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:500
  • Knockout Validated
  • Western Blot 1:5000
Control
HeLa Whole Cell Lysate
Reviewed Applications
Read 3 Reviews rated 4.7
using
NBP1-50627 in the following applications:

Publications
Read Publication using
NBP1-50627 in the following applications:

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 0.1% BSA, 50% Glycerol
Preservative
0.05% Sodium Azide
Concentration
0.61 mg/ml
Purity
Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for GOLM1 Antibody

  • bA379P1.3
  • FLJ23608
  • golgi membrane protein 1
  • Golgi membrane protein GP73
  • Golgi phosphoprotein 2C9orf155
  • golgi protein, 73-kD
  • GOLPH2 FLJ22634
  • GP73 chromosome 9 open reading frame 155
  • GP73
  • PSEC0257

Background

In liver, prostate and lung, both viral and non-viral related disease is correlated to the upregulation of GOLPH2, also known as GOLM1, GP73 or Golgi phosphoprotein 2. While it remains unclear how viral insertions are manifested into the Golgi apparatus and subsequent shepherding of proteins, GOLPH2 seems to be affected by the insertions in such a way that it can be attributed to disease phenotypes. Furthermore, because GOLPH2 is so uniquely regulated in cells, it can be successfully used as a clinically relevant molecular biomarker for cancer. As it is secreted and excreted, GOLPH2 is a useful biomarker because of relative ease of sampling in urine and direct correlation to urine concentrations and disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GOLM1 Antibody (NBP1-50627)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC/IF.


Filter By Application
ICC/IF
(1)
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Filter By Species
Human
(1)
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Reviews for GOLM1 Antibody (NBP1-50627) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.

Reviews using NBP1-50627:
Filter by Applications
Western Blot
(2)
Immunocytochemistry
(1)
All Applications
Filter by Species
Human
(3)
All Species
Images Ratings Applications Species Date Details
Western Blot GOLM1 NBP1-50627
Enlarge
4
reviewed by:
Liyong Zhang
Western Blot Human 12/15/2017
View

Summary

ApplicationWestern Blot
Sample Testedhuman liver and primary hepatocytes
SpeciesHuman
LotA-2

Comments

Commentshuman liver and primary hepatocytes
Immunocytochemistry GOLM1 NBP1-50627
Enlarge
5
reviewed by:
Verified Customer
Immunocytochemistry Human 06/12/2014
View

Summary

ApplicationImmunocytochemistry
SpeciesHuman
Western Blot GOLM1 NBP1-50627
Enlarge
5
reviewed by:
Verified Customer
Western Blot Human 06/12/2014
View

Summary

ApplicationWestern Blot
SpeciesHuman

Product General Protocols

View specific protocols for GOLM1 Antibody (NBP1-50627): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GOLM1 Antibody (NBP1-50627) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for GOLM1 Antibody (NBP1-50627)

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

Liyong Zhang
12/15/2017
Application: Western Blot
Species: Human

Verified Customer
06/12/2014
Application: Immunocytochemistry
Species: Human

Verified Customer
06/12/2014
Application: Western Blot
Species: Human

Bioinformatics

Gene Symbol GOLM1
Entrez
Uniprot