GOLGB1/Giantin Antibody

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Biological Strategies: Western Blot: GOLGB1/Giantin Antibody [NBP2-22321] - Whole cell lysate (50 ug) from 293T, HeLa, and Jurkat cells. NBP2-22321 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an ...read more
Immunoprecipitation: GOLGB1/Giantin Antibody [NBP2-22321] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22321 used for IP at 6 ug/mg lysate. GOLGB1 was also immunoprecipitated ...read more
Immunofluorescence: GOLGB1/Giantin Antibody [NBP2-22321] - Immunofluorescence analytics of untreated MDA-MB-231 cells showed perinuclear colonization of Giantin and other proteins, demonstrating this cell line exhibits ...read more
Immunofluorescence: GOLGB1/Giantin Antibody [NBP2-22321] - Effect of ActD on the perinuclear localization of the Golgi apparatus of MDA-MB-231 cells assessed by immunofluorescence to Giantin, GM130 and TGN46. Cells were ...read more
Immunofluorescence: GOLGB1/Giantin Antibody [NBP2-22321] - Shows the combination effects of antitumor drugs and ARF1 disruptors on the MDA-MB-231 cells Golgi apparatus. Cells were fixed, permeabilized, and immunolabeled ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

GOLGB1/Giantin Antibody Summary

Immunogen
The immunogen this antibody was made to, maps to a region between residue 425 to 475 of human Golgin B1 using the numbering given in entry NP_004478.3 (GeneID 2804).
Marker
Golgi Apparatus Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GOLGB1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000 - 1:10000
  • Immunoprecipitation 2 - 10 ug/mg
  • Immunofluorescence
Control
GOLGB1/Giantin Lysate (NBP2-64628)
Publications
Read Publications using
NBP2-22321 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GOLGB1/Giantin Antibody

  • 372 kDa Golgi complex-associated protein
  • GCP
  • GCP372
  • GCP372GIANTIN
  • Giantin
  • GOLGB1
  • golgi autoantigen, golgin subfamily b, macrogolgin (with transmembrane signal)1,macrogolgin
  • golgi integral membrane protein 1
  • golgin B1
  • golgin B1, golgi integral membrane protein
  • Golgin subfamily B member 1
  • GOLIM1
  • Macrogolgin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GOLGB1/Giantin Antibody (NBP2-22321)(2)

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Video Protocols

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Control Lysate(s)

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Diseases for GOLGB1/Giantin Antibody (NBP2-22321)

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Bioinformatics

Gene Symbol GOLGB1
Uniprot