GOLGB1/Giantin Antibody

Western Blot: GOLGB1/Giantin Antibody [NBP2-22321] - HeLa cell lysate sample treated with DMSO was run on 8% SDS-PAGE. Both giantin monomer and dimer are indicated. Western blot image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: GOLGB1/Giantin Antibody [NBP2-22321] - PC-3 human prostate cancer cell line. Image was captured using EVOS M5000 microscope. Red - giantin; blue - nucleus, DAPI; bar 40 micron. ICC/IF image submitted by a verified customer review.

Western Blot: GOLGB1/Giantin Antibody [NBP2-22321] - Whole cell lysate (50 ug) from 293T, HeLa, and Jurkat cells. NBP2-22321 used for WB at 0.1 ug/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.

Immunocytochemistry/Immunofluorescence: GOLGB1/Giantin Antibody [NBP2-22321] - Untreated MDA-MB-231 cells showed perinuclear colonization of Giantin and other proteins, demonstrating this cell line exhibits the architecture of a typical Golgi apparatus. Cells were fixed, permeabilized, and immunolabeled with rabbit polyclonal antibody to Giantin. Secondary antibodies in other channels. Nuclei were stained with DAPI (gray channel). Fluorescence microscopy was used to examine stained cells. The final row is a merged image form the previous 3 rows. Overlapping of different combinations of rows indicated in different colors. Bar: 10 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29614107/) licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: GOLGB1/Giantin Antibody [NBP2-22321] - Effect of ActD on the perinuclear localization of the Golgi apparatus of MDA-MB-231 cells assessed by immunofluorescence to Giantin, GM130 and TGN46. Cells were fixed, permeabilized, and immunolabeled with rabbit polyclonal antibody to Giantin. The final row is a merged image form the previous 3 rows. Overlapping of different combinations of rows indicated in different colors. Bar: 10 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29614107/) licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: GOLGB1/Giantin Antibody [NBP2-22321] - Shows the combination effects of antitumor drugs and ARF1 disruptors on the MDA-MB-231 cells Golgi apparatus. Cells were fixed, permeabilized, and immunolabeled with rabbit polyclonal antibody to Giantin. The final row is a merged image form the previous 3 rows. Overlapping of different combinations of rows indicated in different colors. Bar: 10 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29614107/) licensed under a CC-BY licence.

Immunoprecipitation: GOLGB1/Giantin Antibody [NBP2-22321] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22321 used for IP at 6 ug/mg lysate. GOLGB1 was also immunoprecipitated by rabbit anti-GOLGB1 antibodies NBP2-22322 and NBP2-22323. For blotting immunoprecipitated GOLGB1, NBP2-22321 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

• Reactivity: Hu
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

GOLGB1/Giantin Antibody Summary

• Immunogen: The immunogen this antibody was made to, maps to a region between residue 425 to 475 of human Golgin B1 using the numbering given in entry NP_004478.3 (GeneID 2804).
• Marker: Golgi Apparatus Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GOLGB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation 2 - 10 ug/mg
  • Western Blot 1:2000 - 1:10000
• Application Notes: Use in ICC/IF reported in scientific literature (PMID: 30453527). GOLGB1/Giantin Antibody is validated for Western blot and ICC/IF from verified customer reviews.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GOLGB1/Giantin Antibody

• 372 kDa Golgi complex-associated protein
• GCP
• GCP372
• GCP372GIANTIN
• Giantin
• GOLGB1
• golgi autoantigen, golgin subfamily b, macrogolgin (with transmembrane signal)1,macrogolgin
• golgi integral membrane protein 1
• golgin B1
• golgin B1, golgi integral membrane protein
• Golgin subfamily B member 1
• GOLIM1
• Macrogolgin

Background

The giantin (GOLGB1) gene codes for a golgin subfamily B member 1 protein that exists in isoform 1 at a length of 3,259 amino acids and a mass of 376 kDa or isoform 2 at 3,269 amino acids long and 377 kDA. GOLGB1 is thought to participate in the development of intercisternal cross-bridges of the Golgi complex. Researchers have investigated its role in hypertension, myopathy, hematopoiesis, encephalitis, and arthritis. GOLGB1 is known to interact with other genes such as PFN2, CAV2, USO1, SLC2A3, and GORASP2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GOLGB1/Giantin Antibody (NBP2-22321)(5)

Publications using NBP2-22321

• Casey CA, Thomes P, Manca S, Petrosyan A. Giantin Is Required for Post-Alcohol Recovery of Golgi in Liver Cells. Biomolecules. 2018-11-16 [PMID: 30453527] (ICC/IF, WB, Human)
• Manca S, Frisbie CP, LaGrange CA et al. The Role of Alcohol-induced Golgi Fragmentation for Androgen Receptor Signaling in Prostate Cancer. Mol. Cancer Res. 2018-09-17 [PMID: 30224543] (ICC/IF, Human)
• Luchsinger C, Aguilar M, Burgos PV et al. Functional disruption of the Golgi apparatus protein ARF1 sensitizes MDA-MB-231 breast cancer cells to the antitumor drugs Actinomycin D and Vinblastine through ERK and AKT signaling. PLoS ONE 2018-04-03 [PMID: 29614107] (Human)
  
  Details:
  Novus Giantin antibody was used in a study looking at the effect of treatment with actinomycin d and vinblastine on the golgi apparatus of MDA-MB-231 cells
• Casey C, Thomes P, Manca S, Petrosyan A. The Role of Golgi Morphology in Post-Alcohol Recovery of Hepatocytes: Observations in Cellular and Animal Models Preprints 2018-10-25 (IP, Human)
• Frisbie CP, Lushnikov AY, Krasnoslobodtsev AV, et al. Post-ER Stress Biogenesis of Golgi Is Governed by Giantin Cells 2019-12-13 [PMID: 31847122] (IP, Human)

Reviews for GOLGB1/Giantin Antibody (NBP2-22321) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Human and PC-3 cells.

reviewed by: Verified Customer IF Human and PC-3 cells 08/19/2020

Summary

• Application: Immunofluorescence
• Sample Tested: PC-3 cells,PC-3 human prostate cancer cell line
• Species: Human and PC-3 cells
• Lot: A1

reviewed by: Verified Customer WB Human 11/20/2018

Summary

• Application: Western Blot
• Sample Tested: HeLa Cell Lysate
• Species: Human
• Lot: A1

Bioinformatics

• Gene Symbol: GOLGB1
• Uniprot:
  • NP_004478.3(Human)