GOLGB1/Giantin Antibody - BSA Free

Western Blot: GOLGB1/Giantin Antibody [NBP2-22321] - HeLa cell lysate sample treated with DMSO was run on 8% SDS-PAGE. Both giantin monomer and dimer are indicated. Western blot image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: GOLGB1/Giantin Antibody [NBP2-22321] - PC-3 human prostate cancer cell line. Image was captured using EVOS M5000 microscope. Red - giantin; blue - nucleus, DAPI; bar 40 micron. ICC/IF image submitted by a verified customer review.

Western Blot: GOLGB1/Giantin Antibody [NBP2-22321] - Whole cell lysate (50 ug) from 293T, HeLa, and Jurkat cells. NBP2-22321 used for WB at 0.1 ug/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.

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Immunoprecipitation: GOLGB1/Giantin Antibody [NBP2-22321] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22321 used for IP at 6 ug/mg lysate. GOLGB1 was also immunoprecipitated by rabbit anti-GOLGB1 antibodies NBP2-22322 and NBP2-22323. For blotting immunoprecipitated GOLGB1, NBP2-22321 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Effect of Golgi disrupting treatments on the Golgi apparatus of MDA-MB-231 cells.Cells were left untreated (A; Control), or transfected to transiently express the HA-epitope-tagged ARF1 constitutively-activated mutant for 16 h (B; HA-ARF1-Q71L), or treated for 60 min either with 5 μg/ml Brefeldin A (C; BFA) or 10 μM Golgicide A (D; GCA). Cells were fixed, permeabilized, and immunolabeled with mouse monoclonal antibody to GM130, rabbit polyclonal antibody to Giantin, and sheep antibody to TGN46. Secondary antibodies were Alexa-594-conjugated donkey anti-mouse IgG (red channel), Alexa-488-conjugated donkey anti-rabbit IgG (green channel), and Alexa-647-conjugated donkey anti-sheep IgG (blue channel). Nuclei were stained with DAPI (gray channel). Stained cells were examined by fluorescence microscopy. Merging red, green, blue, and grey channels generated the fourth image on each row; yellow indicates overlapping localization of the red and green channels, cyan indicates overlapping localization of the green and blue channels, magenta indicates overlapping localization of the red and blue channels, and white indicates overlapping localization of all three channels. Bar, 10 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29614107), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Effect of Actinomycin D and Vinblastine on the Golgi apparatus of MDA-MB-231 cells.Cells were left untreated (A; Control), or treated for 60 min either with 10 ng/ml Actinomycin D (B; ActD) or 25 nM Vinblastine (C; VLB). Cells were fixed, permeabilized, and immunolabeled with mouse monoclonal antibody to GM130, rabbit polyclonal antibody to Giantin, and sheep antibody to TGN46. Secondary antibodies were Alexa-594-conjugated donkey anti-mouse IgG (red channel), Alexa-488-conjugated donkey anti-rabbit IgG (green channel), and Alexa-647-conjugated donkey anti-sheep IgG (blue channel). Nuclei were stained with DAPI (gray channel). Stained cells were examined by fluorescence microscopy. Merging red, green, blue, and grey channels generated the fourth image on each row; yellow indicates overlapping localization of the red and green channels, cyan indicates overlapping localization of the green and blue channels, magenta indicates overlapping localization of the red and blue channels, and white indicates overlapping localization of all three channels. Bar, 10 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29614107), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

GOLGB1/Giantin Antibody - BSA Free Summary

• Immunogen: The immunogen this antibody was made to, maps to a region between residue 425 to 475 of human Golgin B1 using the numbering given in entry NP_004478.3 (GeneID 2804).
• Marker: Golgi Apparatus Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GOLGB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Validated from a verified customer review.
  • Immunoprecipitation 2 - 10 ug/mg
  • Western Blot 1:2000 - 1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GOLGB1/Giantin Antibody - BSA Free

• 372 kDa Golgi complex-associated protein
• GCP
• GCP372
• GCP372GIANTIN
• Giantin
• GOLGB1
• golgi autoantigen, golgin subfamily b, macrogolgin (with transmembrane signal)1,macrogolgin
• golgi integral membrane protein 1
• golgin B1
• golgin B1, golgi integral membrane protein
• Golgin subfamily B member 1
• GOLIM1
• Macrogolgin

Background

The giantin (GOLGB1) gene codes for a golgin subfamily B member 1 protein that exists in isoform 1 at a length of 3,259 amino acids and a mass of 376 kDa or isoform 2 at 3,269 amino acids long and 377 kDA. GOLGB1 is thought to participate in the development of intercisternal cross-bridges of the Golgi complex. Researchers have investigated its role in hypertension, myopathy, hematopoiesis, encephalitis, and arthritis. GOLGB1 is known to interact with other genes such as PFN2, CAV2, USO1, SLC2A3, and GORASP2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GOLGB1/Giantin Antibody (NBP2-22321)(7)

Publications using NBP2-22321

• Amanda JM, Taylor ED, Artem NP et al. Alcohol-induced Golgiphagy is triggered by the downregulation of Golgi GTPase RAB3D Autophagy. 2024-04-09 [PMID: 38591519]
• Amanda M, Artem P, Taylor D et al. Targeting the ATF6-mediated ER stress response and autophagy blocks integrin-driven prostate cancer progression Mol Cancer Res. 2023-06-14 [PMID: 37314749] (Western Blot, Human)
• Casey CA, Thomes P, Manca S, Petrosyan A. Giantin Is Required for Post-Alcohol Recovery of Golgi in Liver Cells. Biomolecules. 2018-11-16 [PMID: 30453527] (WB, ICC/IF, Human)
• Manca S, Frisbie CP, LaGrange CA et al. The Role of Alcohol-induced Golgi Fragmentation for Androgen Receptor Signaling in Prostate Cancer. Mol. Cancer Res. 2018-09-17 [PMID: 30224543] (ICC/IF, Human)
• Luchsinger C, Aguilar M, Burgos PV et al. Functional disruption of the Golgi apparatus protein ARF1 sensitizes MDA-MB-231 breast cancer cells to the antitumor drugs Actinomycin D and Vinblastine through ERK and AKT signaling. PLoS ONE 2018-04-03 [PMID: 29614107] (Human)
  
  Details:
  Novus Giantin antibody was used in a study looking at the effect of treatment with actinomycin d and vinblastine on the golgi apparatus of MDA-MB-231 cells
• Casey C, Thomes P, Manca S, Petrosyan A. The Role of Golgi Morphology in Post-Alcohol Recovery of Hepatocytes: Observations in Cellular and Animal Models Preprints 2018-10-25 (IP, Human)
• Frisbie CP, Lushnikov AY, Krasnoslobodtsev AV, et al. Post-ER Stress Biogenesis of Golgi Is Governed by Giantin Cells 2019-12-13 [PMID: 31847122] (IP, Human)

Reviews for GOLGB1/Giantin Antibody (NBP2-22321) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Human and PC-3 cells.

reviewed by: Armen Petrosyan IF Human and PC-3 cells 08/19/2020

Summary

• Application: Immunofluorescence
• Sample Tested: PC-3 cells,PC-3 human prostate cancer cell line
• Species: Human and PC-3 cells
• Lot: A1

reviewed by: Armen Petrosyan WB Human 11/20/2018

Summary

• Application: Western Blot
• Sample Tested: HeLa Cell Lysate
• Species: Human
• Lot: A1

Bioinformatics

• Gene Symbol: GOLGB1
• Uniprot:
  • NP_004478.3(Human)