ADAM8 Antibody [Unconjugated]

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ADAM8 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human ADAM8 Ectodomain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1031) at 15 µg/mL for 3 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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ADAM8 Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ADAM8 Ectodomain
Glu158-Ser653
Accession # P78325
Specificity
Detects human ADAM8 Ectodomain in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse ADAM8 is observed and less than 1% cross-reactivity with recombinant human (rh) ADAM12, rhADAM19, and rhADAM33 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ADAM8
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 2 Reviews rated 4
using
AF1031 in the following applications:

Publications
Read Publications using
AF1031 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ADAM8 Antibody [Unconjugated]

  • a disintegrin and metalloproteinase domain 8
  • ADAM 8
  • ADAM metallopeptidase domain 8
  • ADAM8
  • CD156a antigen
  • CD156a
  • CD156human leukocyte differentiation antigen
  • Cell surface antigen MS2
  • EC 3.4.24
  • EC 3.4.24.-
  • MGC134985
  • MS2
  • MS2disintegrin and metalloproteinase domain-containing protein 8

Background

ADAM8, also known as cell surface antigen MS2 or CD156a, is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1, 2). ADAM8 can cleave a variety of substrates and has been shown as a sheddase for the low affinity IgE receptor CD23 and the neural recognition molecule CHL1 (3, 4). Expression and regulation studies suggest that ADAM8 is a novel osteoclast stimulating factor and may play a role in asthma (5, 6). The 824 amino acid precursor of human ADAM8 consists of a signal peptide (residues 1 to 16), a pro peptide (residues 17 to 199), a metaloprotease domain (residues 200 to 400), a disintegrin-like domain (residues 408 to 494), a cysteine-rich region (residues 497 to 613), an EGF-like domain (residues 614 to 640), a transmembrane region (residues 656 to 676) and a cytoplasmic domain (residues 677 to 824).

  1. Yoshiyama, K. et al. (1997) Genomics 41:56.
  2. Moss, M.L. and J.W. Bartsch (2004) Biochemistry 43:7227.
  3. Fourie, A.M. et al. (2003) J. Biol. Chem. 278:30469.
  4. Naus, S. et al. (2004) J. Biol. Chem. 279:16083.
  5. Choi, S.J. et al. (2001) J. Bone Miner. Res. 16:814.
  6. King, N.E. et al. (2004) Am. J. Respir. Cell Mol. Biol. 31:257.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ADAM8 Antibody (AF1031)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: Flow Cytometry, IHC, Immunoprecipitation, Western Blot.


Filter By Application
Flow Cytometry
(1)
IHC
(2)
Immunoprecipitation
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(5)
All Species
Showing Publications 1 - 5 of 5.
Publications using AF1031 Applications Species
C Conrad, D Yildiz, SJ Cleary, A Margraf, L Cook, U Schlomann, B Panaretou, JL Bowser, H Karmouty-Q, J Li, NK Berg, SC Martin, A Aljohmani, SF Moussavi-H, KM Wang, JJ Tian, M Magnen, C Valet, L Qiu, JP Singer, HK Eltzschig, CAPSys Stu, W Bertrams, S Herold, N Suttorp, B Schmeck, ZT Ball, A Zarbock, MR Looney, JW Bartsch ADAM8 signaling drives neutrophil migration and ARDS severity JCI Insight, 2022;7(3):. 2022 [PMID: 35132956] (Flow Cytometry, Human) Flow Cytometry Human
C Jaworek, Y Verel-Yilm, S Driesch, S Ostgathe, L Cook, S Wagner, DK Bartsch, EP Slater, JW Bartsch Cohort Analysis of ADAM8 Expression in the PDAC Tumor Stroma Journal of personalized medicine, 2021;11(2):. 2021 [PMID: 33578644] (IHC, Human) IHC Human
K Theodorou, EPC van der Vo, MJ Gijbels, IMJ Wolfs, M Jeurissen, TL Theelen, JC Sluimer, E Wijnands, JP Cleutjens, Y Li, Y Jansen, C Weber, A Ludwig, JF Bentzon, JW Bartsch, EAL Biessen, MMPC Donners Whole body and hematopoietic ADAM8 deficiency does not influence advanced atherosclerotic lesion development, despite its association with human plaque progression Sci Rep, 2017;7(1):11670. 2017 [PMID: 28916789] (IHC, Human) IHC Human
Schlomann U, Koller G, Conrad C, Ferdous T, Golfi P, Garcia A, Hofling S, Parsons M, Costa P, Soper R, Bossard M, Hagemann T, Roshani R, Sewald N, Ketchem R, Moss M, Rasmussen F, Miller M, Lauffenburger D, Tuveson D, Nimsky C, Bartsch J ADAM8 as a drug target in pancreatic cancer. Nat Commun, 2015;6(0):6175. 2015 [PMID: 25629724] (Immunoprecipitation, Human) Immunoprecipitation Human
Stokes A, Joutsa J, Ala-Aho R, Pitchers M, Pennington CJ, Martin C, Premachandra DJ, Okada Y, Peltonen J, Grenman R, James HA, Edwards DR, Kahari VM Expression profiles and clinical correlations of degradome components in the tumor microenvironment of head and neck squamous cell carcinoma. Clin. Cancer Res., 2010;16(0):2022. 2010 [PMID: 20305301] (Western Blot, Human) Western Blot Human

Reviews for ADAM8 Antibody (AF1031) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using AF1031:
Filter by Applications
IHC-Fr
(1)
IHC-P
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen ADAM8 AF1031
Enlarge
5
reviewed by:
PENG SHI
IHC-Fr Human 01/27/2015
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedHuman intervetebral disc nucleus pulposus
SpeciesHuman
LotFUU01
  3
reviewed by:
Anonymous -
IHC-P Human 01/05/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 23101757
SpeciesHuman

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Recent Reviews

4
5
1
4
0
3
1
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1
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PENG SHI
01/27/2015
Application: IHC-Fr
Species: Human

 
Anonymous -
01/05/2015
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol ADAM8
Uniprot