GMFG Antibody

Images

 
Western Blot: GMFG Antibody [NBP2-31367] - WB detection of GMFG target in (A) human spleen lysate, (B) human lungs lysate, and (C) recombinant protein using GMFG antibody. Concentration of primary antibody used: 3 ug/ml ...read more
Immunocytochemistry/ Immunofluorescence: GMFG Antibody [NBP2-31367] - GMFG antibody was tested in HepG2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

GMFG Antibody Summary

Immunogen
GMFG
Predicted Species
Mouse (94%), Rat (91%), Bovine (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GMFG
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 3 ug/ml
  • Immunocytochemistry/Immunofluorescence 0.1 ug/mL
Application Notes
GMFG (glia maturation factor gamma) is a 142 amino acids long protein with predicted molecular weight of 16.8 kDa. In our Western blot validation with human spleen and lung tissue lysates, this GMFG antibody detected a specific band at ~17 kDa and an additional/uncharacterized band at ~45 kDa position.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
16.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human. Based upon the mmunogen sequence similarity, this antibody is predicted to react with Mouse (94%), Rat (91%) and Bovine (97%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
No buffer
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for GMFG Antibody

  • glia maturation factor gamma
  • glia maturation factor, gamma
  • GMF-GAMMA
  • MGC126867

Background

GMFG is a hematopoietic-specific protein that mediates the pluripotentiality and lineage commitment of human hematopoietic stem cells. Glia maturation factor gamma is a cytokine-responsive protein in EPO-induced and G-CSF-induced hematopoietic lineage development. Glia maturation factor also acts as a Nerve Growth Factor in nervous system development, angiogenesis and immune function. GMFG possesses hematopoietic tissue-specific gene expression, a promoter concentrated with high-score hematopoiesis-specific transcription factors, and molecular coevolution with a rudimentary blood/immune system. Glia Maturation Factor-Gamma (GMF-Gamma) Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 142 amino acids and having a total molecular mass of 16.8 kDa. Glia Maturation Factor-Gamma, GMF-Gamma, Human Recombinant is purified by proprietary chromatographic techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GMFG
Entrez
Uniprot