Sin1/MAPKAP1 Antibody

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Western Blot: Sin1/MAPKAP1 Antibody [NB110-40424] - Truncated Sin1/MAPKAP1 displaces endogenous Sin1/MAPKAP1 from mTORC2 in DLD1 colon cancer cells. Sin1/MAPKAP1 constructs incorporate into mTORC2 and displace ...read more
Immunohistochemistry-Paraffin: Sin1/MAPKAP1 Antibody [NB110-40424] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- Sin1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: Sin1/MAPKAP1 Antibody [NB110-40424] - Detection of Human Sin1 on HeLa whole cell lysate.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, Pm, ShSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Sin1/MAPKAP1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 470 and the C-terminus (residue 522) of human stress-activated map kinase interacting protein 1 using the numbering given in entry NP_001006618.1 (GeneID 79109).
Predicted Species
Mouse (100%), Rat (100%), Chicken (100%), Sheep (100%), Primate (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAPKAP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Theoretical MW
59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Whole Cell Lysate
Sin1/MAPKAP1 Overexpression Lysate
Publications
Read Publications using
NB110-40424 in the following applications:

Reactivity Notes

Orangutan (100%).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Sin1/MAPKAP1 Antibody

  • JC310
  • MAPKAP1
  • MEKK2-interacting protein 1
  • MGC2745
  • MIP1
  • MIP1SIN1SIN1b
  • Mitogen-activated protein kinase 2-associated protein 1
  • mitogen-activated protein kinase associated protein 1
  • mSIN1
  • ras inhibitor MGC2745
  • SAPK-interacting protein 1
  • Sin1
  • SIN1g
  • stress-activated map kinase interacting protein 1
  • Stress-activated map kinase-interacting protein 1
  • stress-activated protein kinase-interacting 1
  • target of rapamycin complex 2 subunit MAPKAP1
  • TORC2 subunit MAPKAP1

Background

Sin1 is a novel component of the eukaryotic SAPK pathway. Sin1 and the H3 and H4 histones interact genetically, with the C terminus of Sin1 physically associating with components of the SWI-SNF complex. This interaction is blocked by the N-terminal half of the protein in full length Sin1. It has been proposed that Sin1 acts as a regulatable bridge between the SWI-SNF complex and the nucleosome Sin1 is also required for effective transcription via the AP-1 factor Pap1, but does not prevent its nuclear translocation. Sin1 is a novel component of the eukaryotic SAPK pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, IHC, IP

Publications for Sin1/MAPKAP1 Antibody (NB110-40424)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IF/IHC, WB.


Filter By Application
IF/IHC
(1)
WB
(2)
All Applications
Filter By Species
Human
(2)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB110-40424 Applications Species
Huang Y, Feng G, Cai J et al. Sin1 promotes proliferation and invasion of prostate Cancer cells by modulating mTORC2-AKT and AR signaling cascades Life Sci. 2020-02-20 [PMID: 32088212] (IF/IHC) IF/IHC
Cameron AJM, Veeriah S, Marshall JJT et al. Uncoupling TORC2 from AGC kinases inhibits tumour growth Oncotarget. 2017-10-17 [PMID: 29156676] (WB, Human) WB Human
Wang Q, Zhu J et al. Tumor suppressor Pdcd4 attenuates Sin1 translation to inhibit invasion in colon carcinoma. Oncogene 2017-09-11 [PMID: 28692058] (WB, Human) WB Human
Das F, Ghosh-Choudhury N, Dey N et al. Unrestrained mammalian target of rapamycin complexes 1 and 2 increase expression of phosphatase and tensin homolog deleted on chromosome 10 to regulate phosphorylation of Akt kinase. J Biol Chem 2012-02-01 [PMID: 22184110]
Misra UK, Pizzo SV. Upregulation of mTORC2 activation by the selective agonist of EPAC, 8-CPT-2Me-cAMP, in prostate cancer cells: assembly of a multiprotein signaling complex. J Cell Biochem 2012-05-01 [PMID: 22173835]
Lu M, Wang J, Ives HE et al. mSIN1 protein mediates SGK1 protein interaction with mTORC2 protein complex and is required for selective activation of the epithelial sodium channel. J Biol Chem 2011-09-01 [PMID: 21757730]
Huang J, Wu S, Wu CL et al. Signaling events downstream of mammalian target of rapamycin complex 2 are attenuated in cells and tumors deficient for the tuberous sclerosis complex tumor suppressors. Cancer Res 2009-08-01 [PMID: 19602587]

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Product General Protocols

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MAPKAP1
Entrez
Uniprot