Transportin 1 Antibody (D45) - BSA Free

Images

 
Western Blot: Transportin 1 Antibody (D45) [NB600-1397] - Human lung cancer cell line usig lysates from HCC44 cell line. (N: Nuclear fraction, C: Cytoplasmic fraction). Image from verified customer review.
Immunohistochemistry-Paraffin: Transportin 1 Antibody (D45) [NB600-1397] - IHC analysis of a formalin fixed paraffin embedded tissue section of human pancreas using Transportin 1 antibody (clone D45) at 1:200 dilution. ...read more
Flow (Intracellular): Transportin 1 Antibody (D45) [NB600-1397] - HeLa cells were stained with Transportin 1 NB600-1397 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then ...read more
Western Blot: Transportin 1 Antibody (D45) [NB600-1397] - Whole cell protein from A431, Hek293 and MCF7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. ...read more
Immunohistochemistry-Paraffin: Transportin 1 Antibody (D45) [NB600-1397] - IHC analysis of a formalin fixed paraffin embedded tissue section of human pancreas using Transportin 1 antibody (clone D45) at 1:200 dilution. ...read more

Product Details

Summary
Reactivity Hu, Mu, Bv, Ca, Pm, RbSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP, MA, CyTOF-ready
Clone
D45
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

Transportin 1 Antibody (D45) - BSA Free Summary

Immunogen
Human recombinant His-tagged Transportin 1. [UniProt# Q92973]
Localization
Cytoplasm. Nucleus.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TNPO1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence reported in scientific literature (PMID 9298975)
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500. Use reported in scientific literature (PMID 24647604)
  • Immunoprecipitation 1:10-1:500
  • Microarray
  • Western Blot 0.1-0.2 ug/ml
Application Notes
WB: Detects a band of approximately 93 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. This antibody is CyTOF ready.
Control
HeLa Nuclear Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB600-1397 in the following applications:

Publications
Read Publications using
NB600-1397 in the following applications:

  • 1 publication
  • 1 publication
  • IP
    1 publication
  • WB
    6 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 15738418).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Transportin 1 Antibody (D45) - BSA Free

  • importin beta 2
  • Importin beta-2
  • IPO2
  • karyopherin (importin) beta 2
  • MIP1Karyopherin beta-2
  • MIPM9 region interaction protein
  • transportin 1
  • transportin-1
  • TRNKPNB2importin 2

Background

Heterogeneous nuclear ribonucleoprotein hnRNPA1 is an abundant nuclear protein that plays an important role in pre-mRNA processing and mRNA export from the nucleus. A1 shuttles rapidly between the nucleus and the cytoplasm, and a 38-amino acid domain, M9, serves as the bi-directional transport signal of A1. Recently, a 90-kD protein, transportin, was identified as the mediator of A1 nuclear import. Transportin mediates the nuclear import of additional hnRNP proteins, including hnRNPF. The nuclear localization of A1 is dependent on ongoing RNA polymerase II transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Transportin 1 Antibody (NB600-1397)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC/IF, IHC-P, IP, WB.


Filter By Application
ICC/IF
(1)
IHC-P
(1)
IP
(1)
WB
(6)
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Human
(5)
Mouse
(2)
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Showing Publications 1 - 7 of 7.
Publications using NB600-1397 Applications Species
Fare CM, Rhine K, Lam A et al. A minimal construct of nuclear-import receptor Karyopherin-beta 2 defines the regions critical for chaperone and disaggregation activity The Journal of biological chemistry 2022-12-15 [PMID: 36529289] (WB, Human) WB Human
Vieira NM, Naslavsky MS, Licinio L et al. A defect in the RNA-processing protein HNRPDL causes limb-girdle muscular dystrophy 1G (LGMD1G). Hum. Mol. Genet. 2014-03-18 [PMID: 24647604] (IHC-P, Human) IHC-P Human
Suzuki M, Iijima M, Nishimura A et al. Two separate regions essential for nuclear import of the hnRNP D nucleocytoplasmic shuttling sequence. FEBS J. 2005-08-01 [PMID: 16045768] (WB, Human) WB Human
Allemand E, Guil S, Myers M et al. Regulation of heterogenous nuclear ribonucleoprotein A1 transport by phosphorylation in cells stressed by osmotic shock. Proc Natl Acad Sci U S A. 2005-03-08 [PMID: 15738418] (WB, Mouse) WB Mouse
Kawamura H, Tomozoe Y, Akagi T et al. Identification of the nucleocytoplasmic shuttling sequence of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its interaction with mRNA. J Biol Chem. 2002-01-25 [PMID: 11705999] (WB, Human) WB Human
Siomi MC, Eder PS, Kataoka N et al. Transportin-mediated nuclear import of heterogeneous nuclear RNP proteins. J Cell Biol. 1997-09-22 [PMID: 9298975] (ICC/IF, WB, IP, Human) ICC/IF, WB, IP Human
Korobeynikov V Therapeutic strategies targeting FUS toxicity in amyotrophic lateral sclerosis: from a novel mouse model of disease to a first-in-human study Thesis 2021-01-01 (WB, Mouse) WB Mouse

Review for Transportin 1 Antibody (NB600-1397) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB600-1397:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Transportin 1 NB600-1397
Enlarge
5
reviewed by:
Pauline Jeannot
WB Human 02/06/2019
View

Summary

ApplicationWestern Blot
Sample Testedhuman lung cancer cell line
SpeciesHuman
LotA1

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Transportin 1 Antibody (NB600-1397) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Transportin 1 Products

Research Areas for Transportin 1 Antibody (NB600-1397)

Find related products by research area.

Blogs on Transportin 1.

Transportin 1 and heterogeneous nuclear ribonucleoprotein D (hnRNPD)
Transportin 1, also known as Karyopherin- beta 2 or Importin- beta 2, is part of the beta -karyopherins family, which consists of importins and exportins responsible for the active transport of proteins between the nucleus and cytoplasm.  Transportin 1 is co...  Read full blog post.

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Recent Reviews

5
5
1
4
0
3
0
2
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1
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Pauline Jeannot
02/06/2019
Application: WB
Species: Human

Bioinformatics

Gene Symbol TNPO1
Entrez
Uniprot