Transportin 1 Antibody (D45) - BSA Free

Western Blot: Transportin 1 Antibody (D45) [NB600-1397] - Human lung cancer cell line usig lysates from HCC44 cell line. (N: Nuclear fraction, C: Cytoplasmic fraction). Image from verified customer review.

Immunohistochemistry-Paraffin: Transportin 1 Antibody (D45) [NB600-1397] - IHC analysis of a formalin fixed paraffin embedded tissue section of human pancreas using Transportin 1 antibody (clone D45) at 1:200 dilution. The antibody generated specific nuclear and cytoplasmic signal of Transportin 1 protein in the glandular cells while the connective tissue was largely negative.

Flow (Intracellular): Transportin 1 Antibody (D45) [NB600-1397] - HeLa cells were stained with Transportin 1 NB600-1397 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-mouse secondary antibody.

Western Blot: Transportin 1 Antibody (D45) [NB600-1397] - Whole cell protein from A431, Hek293 and MCF7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Transportin 1 (D45) in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.

Immunohistochemistry-Paraffin: Transportin 1 Antibody (D45) [NB600-1397] - IHC analysis of a formalin fixed paraffin embedded tissue section of human pancreas using Transportin 1 antibody (clone D45) at 1:200 dilution. The antibody generated specific nuclear and cytoplasmic signal of Transportin 1 protein in the glandular cells while the connective tissue was largely negative.

• Reactivity: Hu, Mu, Bv, Ca, Pm, Rb
• Applications: WB, Flow, ICC/IF, IHC, IP, MA, CyTOF-ready
• Clone: D45
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Transportin 1 Antibody (D45) - BSA Free Summary

• Immunogen: Human recombinant His-tagged Transportin 1. [UniProt# Q92973]
• Localization: Cytoplasm. Nucleus.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNPO1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence reported in scientific literature (PMID 9298975)
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500. Use reported in scientific literature (PMID 24647604)
  • Immunoprecipitation 1:10-1:500
  • Microarray
  • Western Blot 0.1-0.2 ug/ml
• Application Notes: WB: Detects a band of approximately 93 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. This antibody is CyTOF ready.
• Control:

  HeLa Nuclear Cell Lysate

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 15738418).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Transportin 1 Antibody (D45) - BSA Free

• importin beta 2
• Importin beta-2
• IPO2
• karyopherin (importin) beta 2
• MIP1Karyopherin beta-2
• MIPM9 region interaction protein
• transportin 1
• transportin-1
• TRNKPNB2importin 2

Background

Heterogeneous nuclear ribonucleoprotein hnRNPA1 is an abundant nuclear protein that plays an important role in pre-mRNA processing and mRNA export from the nucleus. A1 shuttles rapidly between the nucleus and the cytoplasm, and a 38-amino acid domain, M9, serves as the bi-directional transport signal of A1. Recently, a 90-kD protein, transportin, was identified as the mediator of A1 nuclear import. Transportin mediates the nuclear import of additional hnRNP proteins, including hnRNPF. The nuclear localization of A1 is dependent on ongoing RNA polymerase II transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Transportin 1 Antibody (NB600-1397)(9)

Publications using NB600-1397

• Stang TE, Salapa HE, Clarke JWE et al. Heterogeneous Nuclear Ribonucleoprotein A1 Knockdown Alters Constituents of Nucleocytoplasmic Transport Brain Sciences 2024-10-19 [PMID: 39452051]
• Fare CM, Rhine K, Lam A et al. A minimal construct of nuclear-import receptor Karyopherin-beta 2 defines the regions critical for chaperone and disaggregation activity The Journal of biological chemistry 2022-12-15 [PMID: 36529289] (WB, Human)
• Guo L, Kim H J et al. Nuclear-Import Receptors Reverse Aberrant Phase Transitions of RNA-Binding Proteins with Prion-like Domains. Cell 2018-04-19 [PMID: 29677512]
• Vieira NM, Naslavsky MS, Licinio L et al. A defect in the RNA-processing protein HNRPDL causes limb-girdle muscular dystrophy 1G (LGMD1G). Hum. Mol. Genet. 2014-03-18 [PMID: 24647604] (IHC-P, Human)
• Suzuki M, Iijima M, Nishimura A et al. Two separate regions essential for nuclear import of the hnRNP D nucleocytoplasmic shuttling sequence. FEBS J. 2005-08-01 [PMID: 16045768] (WB, Human)
• Allemand E, Guil S, Myers M et al. Regulation of heterogenous nuclear ribonucleoprotein A1 transport by phosphorylation in cells stressed by osmotic shock. Proc Natl Acad Sci U S A. 2005-03-08 [PMID: 15738418] (WB, Mouse)
• Kawamura H, Tomozoe Y, Akagi T et al. Identification of the nucleocytoplasmic shuttling sequence of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its interaction with mRNA. J Biol Chem. 2002-01-25 [PMID: 11705999] (WB, Human)
• Siomi MC, Eder PS, Kataoka N et al. Transportin-mediated nuclear import of heterogeneous nuclear RNP proteins. J Cell Biol. 1997-09-22 [PMID: 9298975] (ICC/IF, WB, IP, Human)
• Korobeynikov V Therapeutic strategies targeting FUS toxicity in amyotrophic lateral sclerosis: from a novel mouse model of disease to a first-in-human study Thesis 2021-01-01 (WB, Mouse)

Review for Transportin 1 Antibody (NB600-1397) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Pauline Jeannot WB Human 02/06/2019

Summary

• Application: Western Blot
• Sample Tested: human lung cancer cell line
• Species: Human
• Lot: A1

Bioinformatics

• Gene Symbol: TNPO1
• Entrez:
  • 3842(Human)
  • 238799(Mouse)
  • 309126(Rat)
• Uniprot:
  • Q92973(Human)