GM-CSF Antibody

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

GM-CSF Antibody Summary

• Description: Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: This whole rabbit serum was prepared by repeated immunizations with full length recombinant human GM-CSF. (Uniprot: P04141)
• Localization: Secreted
• Specificity: In ELISA and other immunoreactive assays, this antiserum will recognize both native and recombinant human GM-CSF in cell supernatants and certain body fluids.
• Isotype: Serum
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CSF2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000-1:5000
  • Immunohistochemistry 1:10-1:500
  • Western Blot 1:500-1:2000
• Application Notes: This antiserum against Human GM-CSF has been tested for use in immunoblotting. Reactivity is also expected in neutralizations, radioimmunoassay, ELISA and immunohistochemistry. This antibody is useful for neutralization of human GM-CSF in bioassays. For neutralization, it is recommended to incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
• Control:

  GM-CSF Overexpression Lysate

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31220744).

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: Antiserum
• Preservative: No Preservative
• Purity: Unpurified

Alternate Names for GM-CSF Antibody

• colony stimulating factor 2 (granulocyte-macrophage)
• Colony-stimulating factor
• CSF
• CSF2
• CSF-2
• GMCSF
• GM-CSF
• GMCSFgranulocyte-macrophage colony-stimulating factor
• granulocyte-macrophage colony stimulating factor
• MGC131935
• MGC138897
• Molgramostim
• molgramostin
• Sargramostim

Background

The cytokine GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GM-CSF Antibody (NB600-632)(5)

Publications using NB600-632

• Saranaruk P, Waraasawapati S, Chamgramol Y et al. Dense GM-CSFR?-expressing immune infiltration is allied with longer survival of intrahepatic cholangiocarcinoma patients PeerJ 2023-03-02 [PMID: 36883059] (Immunohistochemistry, Human)
• Zhang G, Jiang C, Xie N, et al. Treatment with andrographolide sulfonate provides additional benefits to imipenem in a mouse model of Klebsiella pneumoniae pneumonia Biomed. Pharmacother. 2019-06-17 [PMID: 31220744] (Mouse)
• Qi Y, Zhang M, Li H et al. Autophagy inhibition by sustained over-production of IL-6 contributes to arsenic-induced carcinogenesis. Cancer Res. 2014-05-15 [PMID: 24830721] (WB, Human)
• Pylayeva-Gupta Y, Lee KE, Hajdu CH et al. Oncogenic Kras-Induced GM-CSF Production Promotes the Development of Pancreatic Neoplasia Cancer Cell 2012-06-12 [PMID: 22698407] (IF/IHC, Human)
• Bayne LJ, Beatty GL, Jhala N et al. Tumor-derived granulocyte-macrophage colony-stimulating factor regulates myeloid inflammation and T cell immunity in pancreatic cancer Cancer Cell 2012-06-12 [PMID: 22698406] (IF/IHC, Human)

FAQs for GM-CSF Antibody (NB600-632). (Showing 1 - 2 of 2 FAQs).

1. I found #NB600-632 but I worry if the applications of this antibody will be changed too. Could you confirm the antibody and let us know if it can be used for IHC and Neut?
   • NB600-632 is guaranteed for use in IHC but not for neutralization. Both NBP2-12341 and NBP1-42357 are useful for both IHC frozen sections and neutralization applications.
2. We have a customer who is interested in NB600-632 (anti-GM-CSF) for use in IHC and has some questions: 1. Are there any images available? 2. Has the antibody been specifically tested with formalin-fixed tissues? 3. Is there a product specific protocol, or if not, is there a recommended method of antigen retrieval? 4. What tissue type(s) were tested? Is there a recommended positive or negative control tissue?
   • NB600-632 has not yet been tested for IHC, however since it has been successfully used for IHC by our customers in published papers, it is guaranteed for IHC as well. Please check the following references for more details: 1) Bayne LJ, Beatty GL, Jhala N et al. Tumor-derived granulocyte-macrophage colony-stimulating factor regulates myeloid inflammation and T cell immunity in pancreatic cancer Cell 2012 Jun 12 [PMID: 22698406] 2) Pylayeva-Gupta Y, Lee KE, Hajdu CH et al. Oncogenic Kras-Induced GM-CSF Production Promotes the Development of Pancreatic Neoplasia Cancer Cell 2012 Jun 12 [PMID: 22698407]

Bioinformatics

• Gene Symbol: CSF2
• Entrez:
  • 1437(Human)
• Uniprot:
  • P04141(Human)