GM-CSF Antibody (OTI8G5)

Western Blot: GM-CSF Antibody (8G5) [NBP2-46364] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GM-CSF.

Immunohistochemistry: GM-CSF Antibody (8G5) [NBP2-46364] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: GM-CSF Antibody (8G5) [NBP2-46364] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: GM-CSF Antibody (8G5) [NBP2-46364] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: GM-CSF Antibody (8G5) [NBP2-46364] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: GM-CSF Antibody (8G5) [NBP2-46364] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

• Reactivity: Hu
• Applications: WB, IHC
• Clone: OTI8G5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

GM-CSF Antibody (OTI8G5) Summary

• Description: Novus Biologicals Mouse GM-CSF Antibody (OTI8G5) (NBP2-46364) is a monoclonal antibody validated for use in IHC and WB. Anti-GM-CSF Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 18-144 of human CSF2(NP_000749) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CSF2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Application Notes: Use in IHC-P reported in scientific literature (PMID:32039403).
• Theoretical MW: 14.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GM-CSF Antibody (OTI8G5)

• colony stimulating factor 2 (granulocyte-macrophage)
• Colony-stimulating factor
• CSF
• CSF2
• CSF-2
• GMCSF
• GM-CSF
• GMCSFgranulocyte-macrophage colony-stimulating factor
• granulocyte-macrophage colony stimulating factor
• MGC131935
• MGC138897
• Molgramostim
• Molgramostin
• Sargramostim

Background

The cytokine GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GM-CSF Antibody (NBP2-46364)(1)

Publication using NBP2-46364

• Tan-Garcia A, Lai F, Sheng Yeong J Liver fibrosis and CD206+ macrophage accumulation are suppressed by anti-GM-CSF therapy JHEP Reports 2019-12-01 [PMID: 32039403] (IF/IHC, IHC-P, Human)

Bioinformatics

• Gene Symbol: CSF2