Glypican 3 Overexpression Lysate (Native)


Western Blot: Glypican 3 Overexpression Lysate (Adult Normal) [NBP2-10593] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Glypican 3.

Product Details

Applications WB

Order Details

Glypican 3 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_004475. The protein contains a C-terminal DDK tag.
Homo sapiens Glypican 3, mRNA.


Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
63.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for Glypican 3

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Glypican 3 Overexpression Lysate (Native)

  • DGSX
  • Glypican 3
  • glypican proteoglycan 3
  • glypican-3
  • GPC3
  • GTR2-2
  • heparan sulphate proteoglycan
  • Intestinal protein OCI-5
  • MXR7
  • OCI5
  • OCI-5
  • secreted glypican-3
  • SGB
  • SGBS


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu
Applications: WB, Simple Western, CyTOF-ready, ICC, ICFlow
Species: Hu
Applications: Flow
Species: Hu
Applications: WB, IHC
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: Flow, CyTOF-reported
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF
Species: Hu, Pm
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Eq, Pm
Applications: WB, IHC-P
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, Simple Western, IHC
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC
Applications: WB

Publications for Glypican 3 Lysate (NBP2-10593) (0)

There are no publications for Glypican 3 Lysate (NBP2-10593).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Glypican 3 Lysate (NBP2-10593) (0)

There are no reviews for Glypican 3 Lysate (NBP2-10593). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Glypican 3 Lysate (NBP2-10593) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Glypican 3 Products

Related Products by Gene

Bioinformatics Tool for Glypican 3 Lysate (NBP2-10593)

Discover related pathways, diseases and genes to Glypican 3 Lysate (NBP2-10593). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Glypican 3 Lysate (NBP2-10593)

Discover more about diseases related to Glypican 3 Lysate (NBP2-10593).

Pathways for Glypican 3 Lysate (NBP2-10593)

View related products by pathway.

PTMs for Glypican 3 Lysate (NBP2-10593)

Learn more about PTMs related to Glypican 3 Lysate (NBP2-10593).

Blogs on Glypican 3

There are no specific blogs for Glypican 3, but you can read our latest blog posts.

Contact Information

Product PDFs

Review this Product

Be the first to review our Glypican 3 Overexpression Lysate (Native) and receive a gift card or discount.


Gene Symbol GPC3

Customers Who Bought This Also Bought