Glypican 3 Antibody - Humanized, (Research Grade Codrituzumab Biosimilar) - Low Endotoxin, Azide and BSA Free

Glypican 3 Antibody (Codrituzumab) - Humanized induced ADCC activity was evaluated using human GPC3 CHOS Cell. The max Lysis rate was approximately 30%.

The endocytosis ratio codrituzumab by HuH-7 increased with the increase of antibody concentration, and the Internalization Rate (%) reached 40%at antibody concentration of 1 nM.

Immobilized human GPC3, His Tag at 2 ug/mL can bind Glypican 3 Antibody (Codrituzumab) - Humanized, EC50=0.008741 ug/mL.

HEPG2 cells were stained with Glypican 3 Antibody (Codrituzumab) - Humanized and negative control protein respectively, washed and then followed by PE and analyzed with FACS.

HEP3B cells were stained with Glypican 3 Antibody (Codrituzumab) - Humanized and negative control protein respectively, washed and then followed by PE and analyzed with FACS.

Human GPC3 CHOS cells were stained with Glypican 3 Antibody (Codrituzumab) - Humanized and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC50=2.142 nM.

Huh-7 cells were stained with Glypican 3 Antibody (Codrituzumab) - Humanized and negative control protein respectively, washed and then followed by PE and analyzed with FACS

• Reactivity: Hu
• Applications: ELISA, Flow, Func
• Clonality: Monoclonal
• Host: Human
• Conjugate: Unconjugated
• Format: Low Endotoxin, Azide and BSA Free
• Concentration: LYOPH

Glypican 3 Antibody - Humanized, (Research Grade Codrituzumab Biosimilar) - Low Endotoxin, Azide and BSA Free Summary

• Description: The heavy chain type is huIgG1, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.
  
  Also known as 'codrituzumab'.
  
  This product is shipped at ambient temperature. Upon receipt, store immediately at -20C or lower for 12 months in a lyophilized state. - 80C for 3 months after reconstitution. Avoid repeated freeze-thaw cycles.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: GPC3 / Glypican-3
• Source: CHO
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Human
• Gene: GPC3
• Purity: Protein A purified
• Endotoxin Note: < 0.001EU/ug,determined by LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Functional

Packaging, Storage & Formulations

• Storage: Store at -20C in powder form. Store at -80C once reconstituted.
• Buffer: Lyophilized from 25mM histidine, 8% sucrose, 0.01% Tween80 (pH6.2)
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Reconstitute with sterile, distilled water to a final concentration of 1 mg/ml. Gently shake to solubilize completely. Do not vortex.

Alternate Names for Array

• DGSX
• Glypican 3
• glypican proteoglycan 3
• glypican-3
• GPC3
• GTR2-2
• heparan sulphate proteoglycan
• Intestinal protein OCI-5
• MXR7
• OCI5
• OCI-5
• secreted glypican-3
• SGB
• SGBS
• SGBS1SDYS

Background

The Glypican 3 (GPC3) gene encodes for a member of the glypican-related integral membrane proteoglycan family (GRIPS). Two isoforms of the protein exist, isoform 1 at a length of 580 amino acids long and 65 kDA while isoform 2 is 526 amino acids long at 59 kDA. The protein encoded by GPC3 is thought to prevent the dipeptidyl peptidase activity of DPP4 and may participate in the suppression and regulation of growth in the predominately mesodermal tissues and organs. The GPC3 gene participates in the Wnt signaling pathway, metabolism, and visual phototransduction through interactions with genes FGF2, WNT3A, WNT7B, BCAN, and BGN. GPC3 has been researched in various diseases such as paine syndrome, horner's sundrome, liver cirrhosis, simpson-golabi-behmel syndrome, germ cell tumors, wilms tumors, complex regional pain syndrome, and hepatocellulcar carcinoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GPC3
• Uniprot:
  • P51654()