Goat anti-Human IgG Fc Secondary Antibody

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, EM, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

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Goat anti-Human IgG Fc Secondary Antibody Summary

Immunogen
Human IgG-Fc Fragment
Specificity
By immunoelectrophoresis and ELISA this reacts specifically with human IgG. This may cross react with IgG from other species.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:30000
  • Electron Microscopy 1:1000 - 1:30000
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:2000
  • Immunohistochemistry 1:200 - 1:2000
  • Immunohistochemistry-Paraffin 1:200 - 1:2000
Application Notes
ELISA, Immunohistochemistry-Paraffin, Western Blot and Immunocytochemistry
Reviewed Applications
Read 1 Review rated 5
using
NB7446 in the following applications:

Publications
Read Publications using
NB7446 in the following applications:

Reactivity Notes

Primate reactivity reported in scientific literature (PMID: 25415380)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Phosphate Buffered Saline (PBS)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Notes

Antiserum was solid phase adsorbed to ensure class specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG. Cross reactivity with IgA, IgM and light chains is less than 0.1%. This antibody may cross react with IgG from other species.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG Fc Antibody (NB7446)(12)

We have publications tested in 1 confirmed species: Primate.

We have publications tested in 1 application: IHC-P.


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IHC-P
(1)
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Filter By Species
Primate
(1)
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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NB7446 Applications Species
Ludinsky M, Christner S, Su N et al. The effects of VEGF-A-inhibitors aflibercept and ranibizumab on the ciliary body and iris of monkeys. Graefes Arch. Clin. Exp. Ophthalmol. 2016 Apr 22 [PMID: 27106625]
Tschulakow A, Christner S, Julien S et al. Effects of a single intravitreal injection of aflibercept and ranibizumab on glomeruli of monkeys. PLoS OnE. 2014 Nov 22 [PMID: 25415380] (IHC-P, Primate) IHC-P Primate
Watanabe TM, Tsukasaki Y, Fujita H et al. Distinct modulated pupil function system for real-time imaging of living cells. PLoS One. 2012 [PMID: 22962597]

Details:
Citation using the FITC form of this antibody.
Friberg H, Jaiswal S, West K et al. Analysis of human monoclonal antibodies generated by dengue virus-specific memory B cells. Viral Immunol. [PMID: 22934599]

Details:
Citation using the HRP form of this antibody.
Khan MN, Sharma SK, Filkins LM et al. PcpA of Streptococcus pneumoniae mediates adherence to nasopharyngeal and lung epithelial cells and elicits functional antibodies in humans. Microbes Infect. [PMID: 22796387]

Details:
Citation using the HRP form of this antibody.
Adawi A, Neville LF. Colony to colorimetry in 6 h: ELISA detection of a surface-expressed Pseudomonas aeruginosa virulence factor using immobilized bacteria. Diagn Microbiol Infect Dis. [PMID: 22726527]

Details:
Citation using the HRP form of this antibody.
Dodge ME, Moon J, Tuladhar R et al. Diverse chemical scaffolds support direct inhibition of the membrane-bound O-acyltransferase porcupine. J Biol Chem 2012 Jun [PMID: 22593577]
Vitale LA, He LZ, Thomas LJ et al. Development of a human monoclonal antibody for potential therapy of CD27-expressing lymphoma and leukemia. Clin Cancer Res. [PMID: 22589397]

Details:
Citation using the FITC form of this antibody.
Messingham KN, Srikantha R, DeGueme AM et al. FcR-independent effects of IgE and IgG autoantibodies in bullous pemphigoid. J Immunol. [PMID: 21646296]

Details:
Citation using the FITC form of this antibody.
Braselmann S, Taylor V, Zhao H et al. R406, an orally available spleen tyrosine kinase inhibitor blocks fc receptor signaling and reduces immune complex-mediated inflammation. J Pharmacol Exp Ther 2006 Dec [PMID: 16946104]
Show All 12 Publications.

Review for IgG Fc Antibody (NB7446) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: IF.

Reviews using NB7446:
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IF
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Immunofluorescence IgG Fc NB7446
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reviewed by:
Natalie Erdmann
IF Human 09/06/2016
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Summary

ApplicationImmunofluorescence
Sample TestedMultiple myeloma cells lines LP-1 and MY5 cytospins
SpeciesHuman
LotA9

Blocking

Blocking Details10% donkey serum in PBS 1 hour at room temperature

Primary Anitbody

Dilution Ratio1/800 1ary antibody diluted in 10% donkey serum/PBS incubated O/N at 4oC.

Secondary Antibody

Secondary DescriptionDonkey F(ab")2 anti-goat IgG (H+L) Cy3
Secondary Manufacturer Cat#Jackson #705-166-147
Secondary Concentration1/2000

Details

Detection NotesZeiss AxioImager, Cy3 filter cube for 100 ms exposure
Fixation DetailsImmersion-fixed in 4% paraformaldehyde 10 minutes, 0.5% Triton X-100 5 minutes permeabilization at room temperature
Wash Description3X 5 minutes PBS after each step

Comments

CommentsLP-1 positive control, MY5 negative control. High background on LP-1 slides, could use higher dilution.

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Recent Reviews

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Natalie Erdmann
09/06/2016
Application: IF
Species: Human