Glypican 3 Antibody (307801) [Unconjugated]

Western blot shows lysates of mouse placenta tissue, mouse lung tissue, mouse adrenal gland tissue, rat placenta tissue, and rat lung tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Glypican 3 Monoclonal Antibody (Catalog # MAB2119) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Glypican 3 at approximately 65-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Glypican 3 Monoclonal Antibody (Catalog # MAB2119) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Glypican 3 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Glypican 3 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Glypican 3 Monoclonal Antibody (Catalog # MAB2119) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, CyTOF-ready, ICC/IF
• Clone: 307801
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Glypican 3 Antibody (307801) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Glypican 3
  Gln25-Val558
  Accession # P51654.1
• Specificity: Detects human Glypican 3 in ELISAs. Deteacts human, mouse, and rat Glypican 3 in Western blots. Does not cross-react with recombinant human (rh) Glypican-2, rhGlypican-5, or rhGlypican-6.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: GPC3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 3-25 ug/mL
  • Western Blot 1-2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glypican 3 Antibody (307801) [Unconjugated]

• DGSX
• Glypican 3
• glypican proteoglycan 3
• glypican-3
• GPC3
• GTR2-2
• heparan sulphate proteoglycan
• Intestinal protein OCI-5
• MXR7
• OCI5
• OCI-5
• secreted glypican-3
• SGB
• SGBS
• SGBS1SDYS

Background

Glypicans (GPC) are a family of heparan sulfate proteoglycans that are attached to the cell surface by a glycosylphosphatidylinositol (GPI) anchor. Six members of this family have been identified in mammals (GPC1-GPC6). All glypican core proteins contain an N-terminal signal peptide, a large globular cysteine-rich domain (CRD) with 14 invariant cysteine residues, a stalk-like region containing the heparan sulfate attachment sites, and a C-terminal GPI attachment site. While glypican proteins do not share strong amino acid sequence identity (they range from 17-63%), the conserved cysteine residues in their CRDs suggests similarity in their three‑dimensional structure (1, 2).

Mutations in GPC3 cause a rare disorder in humans, Simpson-Golabi-Behmel Syndrome, which is characterized by pre and postnatal overgrowth of multiple tissues and organs and an increased risk for developing embryonic tumors (3). These features are also present in the mouse knock-out of GPC3 indicating that GPC3 regulates cell survival and inhibits cell proliferation during development (4). Glypican 3 has been implicated in regulating many different signaling pathways including: IGF, FGF, BMP, and Wnt. An endoproteolytic processing of GPC3 by proprotein convertases is required for the modulation of Wnt signaling (5). Direct interaction with FGF-basic has been observed and is mediated by the heparan sulfate chains (6).

1. Filmus, J. and S.B. Selleck (2001) J. Clinical Invest. 108:497. 
2. De Cat, B and G. David (2001) Seminars in Cell & Dev. Biol. 12:117. 
3. Pilia, G. et al. (1996) Nat. Genet. 12: 241. 
4. Cano-Gauci, D.F. et al. (1999) J. Cell Biol. 146: 255.
5. De Cat, B. et al. (2003) J. Cell Biol. 163:625.
6. Song, H.H. et al. (1997) J. Biol. Chem. 272:7574.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glypican 3 Antibody (MAB2119)(5)

Publications using MAB2119

• Zhu, M;Wu, Y;Zhu, T;Chen, J;Chen, Z;Ding, H;Tan, S;He, J;Zeng, Q;Huang, X; Multifunctional bispecific nanovesicles targeting SLAMF7 trigger potent antitumor immunity Cancer immunology research 2024-05-31 [PMID: 38819238] (Flow Cytometry, Western Blot, Mouse)
• Xiao-Hong Ma, Kun Chen, Shuang Wang, Si-Yun Liu, Deng-Feng Li, Yong-Tao Mi et al. Bi-specific T1 positive-contrast-enhanced magnetic resonance imaging molecular probe for hepatocellular carcinoma in an orthotopic mouse model World Journal of Gastrointestinal Oncology 4/15/2022 [PMID: 35582105]
• XH Ma, S Wang, SY Liu, K Chen, ZY Wu, DF Li, YT Mi, LB Hu, ZW Chen, XM Zhao Development and in vitro study of a bi-specific magnetic resonance imaging molecular probe for hepatocellular carcinoma World J. Gastroenterol., 2019-06-28;25(24):3030-3043. 2019-06-28 [PMID: 31293339] (Flow Cytometry, Mouse)
• P Hu, B Cheng, Y He, Z Wei, D Wu, Z Meng Autophagy suppresses proliferation of HepG2 cells via inhibiting glypican-3/wnt/?-catenin signaling Onco Targets Ther, 2018-01-04;11(0):193-200. 2018-01-04 [PMID: 29379301] (Western Blot, Human)
• Haupt LM, Murali S, Mun FK, Teplyuk N, Mei LF, Stein GS, van Wijnen AJ, Nurcombe V, Cool SM The heparan sulfate proteoglycan (HSPG) glypican-3 mediates commitment of MC3T3-E1 cells toward osteogenesis. J. Cell. Physiol., 2009-09-01;220(3):780-91. 2009-09-01 [PMID: 19479939] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: GPC3
• Uniprot:
  • P51654.1()