Glypican 3 Antibody (307801) [Allophycocyanin]

HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Glypican 3 APC-conjugated Monoclonal Antibody (Catalog # FAB2119A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 307801
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

Glypican 3 Antibody (307801) [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Glypican 3
  Gln25-Val558
  Accession # P51654.1
• Specificity: Detects human Glypican 3 in ELISAs and Western blots. Does not cross-react with recombinant human (rh) Glypican-2, rhGlypican-5, or rhGlypican-6.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: GPC3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glypican 3 Antibody (307801) [Allophycocyanin]

• DGSX
• Glypican 3
• glypican proteoglycan 3
• glypican-3
• GPC3
• GTR2-2
• heparan sulphate proteoglycan
• Intestinal protein OCI-5
• MXR7
• OCI5
• OCI-5
• secreted glypican-3
• SGB
• SGBS
• SGBS1SDYS

Background

Glypicans (GPC) are a family of heparan sulfate proteoglycans that are attached to the cell surface by a glycosylphosphatidylinositol (GPI) anchor. Six members of this family have been identified in mammals (GPC1-GPC6). All glypican core proteins contain an N-terminal signal peptide, a large globular cysteine-rich domain (CRD) with 14 invariant cysteine residues, a stalk-like region containing the heparan sulfate attachment sites, and a C-terminal GPI attachment site. While glypican proteins do not share strong amino acid sequence identity (they range from 17-63%), the conserved cysteine residues in their CRDs suggests similarity in their three‑dimensional structure (1, 2).

Mutations in GPC3 cause a rare disorder in humans, Simpson-Golabi-Behmel Syndrome, which is characterized by pre and postnatal overgrowth of multiple tissues and organs and an increased risk for developing embryonic tumors (3). These features are also present in the mouse knock-out of GPC3 indicating that GPC3 regulates cell survival and inhibits cell proliferation during development (4). Glypican 3 has been implicated in regulating many different signaling pathways including: IGF, FGF, BMP, and Wnt. An endoproteolytic processing of GPC3 by proprotein convertases is required for the modulation of Wnt signaling (5). Direct interaction with FGF-basic has been observed and is mediated by the heparan sulfate chains (6).

1. Filmus, J. and S.B. Selleck (2001) J. Clinical Invest. 108:497. 
2. De Cat, B and G. David (2001) Seminars in Cell & Dev. Biol. 12:117. 
3. Pilia, G. et al. (1996) Nat. Genet. 12: 241. 
4. Cano-Gauci, D.F. et al. (1999) J. Cell Biol. 146: 255.
5. De Cat, B. et al. (2003) J. Cell Biol. 163:625.
6. Song, H.H. et al. (1997) J. Biol. Chem. 272:7574.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glypican 3 Antibody (FAB2119A)(6)

Publications using FAB2119A

• Thangaraj, JL;Coffey, M;Lopez, E;Kaufman, DS; Disruption of TGF-? signaling pathway is required to mediate effective killing of hepatocellular carcinoma by human iPSC-derived NK cells Cell stem cell 2024-07-01 [PMID: 38986609] (Flow Cytometry, Human)
• Ewelina Rodakowska, Aurelia Walczak‑Drzewiecka, Marta Borowiec, Michal Gorzkiewicz, Joanna Grzesik, Marcin Ratajewski et al. Recombinant immunotoxin targeting GPC3 is cytotoxic to H466 small cell lung cancer cells Oncology Letters 2021-01-21 [PMID: 33613711]
• L Man, N Lustgarten, E Kallinos, Z Redhead-La, S Liu, B Schattman, D Redmond, K Hancock, N Zaninovic, G Schattman, Z Rosenwaks, D James Comparison of Human Antral Follicles of Xenograft versus Ovarian Origin Reveals Disparate Molecular Signatures Cell Rep, 2020-08-11;32(6):108027. 2020-08-11 [PMID: 32783948] (Flow Cytometry, ICC, IHC, Human)
• M Hamaoka, T Kobayashi, Y Tanaka, H Mashima, H Ohdan Clinical significance of glypican-3-positive circulating tumor cells of hepatocellular carcinoma patients: A prospective study PLoS ONE, 2019-05-29;14(5):e0217586. 2019-05-29 [PMID: 31141571] (Cell Isolation, Human)
• D D'Avola, C Villacorta, SN Martins-Fi, A Craig, I Labgaa, J von Felden, A Kimaada, A Bonaccorso, P Tabrizian, BM Hartmann, R Sebra, M Schwartz, A Villanueva High-density single cell mRNA sequencing to characterize circulating tumor cells in hepatocellular carcinoma Sci Rep, 2018-08-01;8(1):11570. 2018-08-01 [PMID: 30068984] (Flow Cytometry, Human)
• F Cartier, E Indersie, S Lesjean, J Charpentie, KB Hooks, A Ghousein, A Desplat, N Dugot-Sena, V Trézéguet, F Sagliocco, M Hagedorn, CF Grosset New tumor suppressor microRNAs target glypican-3 in human liver cancer Oncotarget, 2017-06-20;0(0):. 2017-06-20 [PMID: 28476031] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: GPC3
• Uniprot:
  • P51654.1()