ABAT Antibody

Images

 
Western Blot: ABAT Antibody [NBP2-21598] - Sample (20 ug of whole cell lysate) A: mouse brain 7. 5% SDS PAGE gel, diluted at 1:5000.
Immunocytochemistry/ Immunofluorescence: ABAT Antibody [NBP2-21598] - Immunofluorescence analysis of methanol-fixed Hep G2, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: ABAT Antibody [NBP2-21598] - Mouse kidney.
Western Blot: ABAT Antibody [NBP2-21598] - 30 ug SK-N-SH whole cell lysate/extract 7. 5 % SDS-PAGE ABAT antibody dilution: 1:1000.
Western Blot: ABAT Antibody [NBP2-21598] - 50 ug Rat brain lysate/extract 7. 5 % SDS-PAGE ABAT antibody dilution: 1:1000.
Immunohistochemistry-Paraffin: ABAT Antibody [NBP2-21598] - Antibody detects ABAT protein at cytosol on U373 xenograft by immunohistochemical analysis. Antibody dilution: 1:500.
Immunohistochemistry-Paraffin: ABAT Antibody [NBP2-21598] - Mouse liver.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

ABAT Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human ABAT. The exact sequence is proprietary.
Localization
Mitochondrion matrix
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ABAT
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:20000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ABAT Antibody

  • (S)-3-amino-2-methylpropionate transaminase
  • 4-aminobutyrate aminotransferase
  • 4-aminobutyrate aminotransferase, mitochondrial
  • 4-aminobutyrate transaminase
  • EC 2.6.1.19
  • EC 2.6.1.22
  • FLJ17813
  • FLJ30272
  • G
  • GABA aminotransferase
  • GABA transaminase
  • GABA transferase
  • GABA-AT
  • GABA-T
  • GABATNPD009
  • Gamma-amino-N-butyrate transaminase
  • hCG1984265
  • L-AIBAT

Background

4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ABAT
Uniprot