ABAT Antibody

Images

 
Western Blot: ABAT Antibody [NBP2-21598] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ABAT antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody ...read more
Immunocytochemistry/ Immunofluorescence: ABAT Antibody [NBP2-21598] - HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: ABAT protein stained by ABAT antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Frozen: ABAT Antibody [NBP2-21598] - Frozen sectioned adult mouse retina. Green: ABAT protein stained by ABAT antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 ...read more
Western Blot: ABAT Antibody [NBP2-21598] - 30 ug SK-N-SH whole cell lysate/extract 7. 5 % SDS-PAGE ABAT antibody dilution: 1:1000.
Immunohistochemistry-Paraffin: ABAT Antibody [NBP2-21598] - Mouse liver. ABAT antibody dilution: 1:500.
Immunohistochemistry-Paraffin: ABAT Antibody [NBP2-21598] - Mouse kidney. ABAT antibody dilution: 1:500.
Immunohistochemistry-Paraffin: ABAT Antibody [NBP2-21598] - Rat brain. ABAT antibody diluted at 1:500.
Immunohistochemistry-Frozen: ABAT Antibody [NBP2-21598] - Frozen sectioned E13.5 Rat brain. Green: ABAT protein stained by ABAT antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, BvSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

ABAT Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human ABAT. The exact sequence is proprietary.
Localization
Mitochondrion matrix
Predicted Species
Porcine (93%), Bovine (95%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ABAT
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:10000
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-21598.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for ABAT Antibody

  • (S)-3-amino-2-methylpropionate transaminase
  • 4-aminobutyrate aminotransferase
  • 4-aminobutyrate aminotransferase, mitochondrial
  • 4-aminobutyrate transaminase
  • ABAT
  • EC 2.6.1.19
  • EC 2.6.1.22
  • FLJ17813
  • FLJ30272
  • G
  • GABA aminotransferase
  • GABA transaminase
  • GABA transferase
  • GABA-AT
  • GABAT
  • GABA-T
  • GABATNPD009
  • Gamma-amino-N-butyrate transaminase
  • hCG1984265
  • L-AIBAT

Background

4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for ABAT Antibody (NBP2-21598)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ABAT
Uniprot