Glutaminase Antibody (GT1075) - Azide and BSA Free

Western Blot: Glutaminase Antibody (GT1075) [NBP3-13512] - Glutaminase (GAC) antibody detects Glutaminase (GAC) protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glutaminase (GAC) antibody (NBP3-13512) diluted at a dilution of 1:1000.

Glutaminase C antibody [GT1075] detects Glutaminase C protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Glutaminase C protein stained by Glutaminase C antibody [GT1075] (NBP3-13512) diluted at 1:400.Blue: Hoechst 33342 staining.

• Reactivity: Hu, Bv
• Applications: WB, ICC/IF
• Clone: GT1075
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Glutaminase Antibody (GT1075) - Azide and BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Glutaminase (GAC). The exact sequence is proprietary.
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: GLS
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 65 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Glutaminase Antibody (GT1075) - Azide and BSA Free

• AAD20
• EC 3.5.1.2
• GLS
• GLS1DKFZp686O15119
• glutaminase C
• glutaminase kidney isoform, mitochondrial
• Glutaminase
• glutaminase, phosphate-activated
• K-Glutaminase
• KIAA0838FLJ10358
• L-Glutamine Amidohydrolase

Background

Sahai (1983) [PubMed 6825316] demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter (Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GLS