Glut3 Antibody - BSA Free

Western Blot: Glut3 Antibody [NBP3-12885] - Unboiled non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glut3 antibody (NBP3-12885) diluted at 1:2000.

Immunocytochemistry/Immunofluorescence: Glut3 Antibody [NBP3-12885] - Glut3 antibody detects Glut3 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Glut3 stained by Glut3 antibody (NBP3-12885) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.

Western Blot: Glut3 Antibody [NBP3-12885] - Unboiled U87-MG whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glut3 antibody (NBP3-12885) diluted at 1:500. (WCE: whole cell extract; ME: membrane extract)

Western Blot: Glut3 Antibody [NBP3-12885] - Unboiled HepG2 whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glut3 antibody (NBP3-12885) diluted at 1:500. (WCE: whole cell extract; ME: membrane extract)

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Glut3 Antibody - BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Glut3. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLC2A3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry Free-Floating Assay dependent
  • Western Blot 1:500-1:3000
• Application Notes: WB As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Glut3 Antibody - BSA Free

• FLJ90380
• Glut3
• GLUT-3
• GLUT3Glucose transporter type 3, brain
• SLC2A3
• solute carrier family 2 (facilitated glucose transporter), member 3
• solute carrier family 2, facilitated glucose transporter member 3

Background

Glucose is the major source of our energy and there are numerous isoforms of the glucose transporter in mammals, including Glut1, Glut2, Glut3, Glut4, Glut5, Glut6, Glut7, Glut8 and Glut9. The Glut5 gene located on the short arm of human chromosome 1 encodes a 501-amino acid facilitative glucose transporter. Glut5 mRNA is highly expressed in small intestine and to a lesser extent in kidney, skeletal muscle and adipose tissue. Glut5 plays a critical role in fructose absorption in the small intestine and its expression is highly induced when exposed to a fructose-enriched diet. Glut5 transporter expressed in human skeletal muscle is specifically localized to the plasma membrane, where it participates in regulating hexose transfer across the sarcolemma. Glut8, a novel glucose transporter-like protein, exhibits significant sequence similarity with the other members of sugar transporter family. Glut8 comprises 12 putative membrane-spanning helices and several conserved motifs, which are important for transport activity. In human tissues, Glut8 is predominantly expressed in testis and, to a lesser extent, in most other tissues including skeletal muscle, heart, small intestine and brain. In addition, the Glut8 glucose transport facilitator has a hormonally regulated testicular function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC2A3