| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89762-25ul | |||
MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." alt="NS0 mouse myeloma cell line transfected with human Glut3 was stained with Mouse Anti-Human Glut3 Monoclonal Antibody (Catalog # MAB1415, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1415-SP | |||
| Carrier Free | FAB1415V-100UG | |||
| Carrier Free | FAB1415U-100UG | |||
| Carrier Free | FAB1415T-100UG | |||
| Carrier Free | FAB1415S-100UG | |||
| Carrier Free | FAB1415R-100UG | |||
| Carrier Free | FAB1415N-100UG | |||
| Carrier Free | FAB1415G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26317-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36485-0.025ml | |||
NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for 
![Flow Cytometry: Glut3 Antibody (JA50-31) [NBP2-66872] - Analysis of SH-SY5Y cells with Glucose Transporter GLUT3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/Glut3-Antibody-JA50-31-Flow-Cytometry-NBP2-66872-img0005.jpg)
![Western Blot: Glut3 Antibody (JA50-31) [NBP2-66872] - Analysis of Glucose Transporter GLUT3 on different lysates with Rabbit anti-Glucose Transporter GLUT3 antibody at 1/1,000 dilution.Lane 1: HepG2-si NT cell lysate (10 ug/Lane)Lane 2: HepG2-si Glucose Transporter GLUT3 cell lysate (10 ug/Lane)Predicted band size: 54 kDaObserved band size: 54 kDaExposure time: 1 minute; ECL: merk4-20% SDS-PAGE gel.NBP2-66872 was shown to specifically react with Glucose Transporter GLUT3 in HepG2-si NT cells. Weakened band was observed when HepG2-si Glucose Transporter GLUT3 sample was tested. HepG2-si NT and HepG2-si Glucose Transporter GLUT3 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (1/1,000) and Loading control antibody (Rabbit anti-GAPDH, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-66872_rabbit-monoclonal-glut3-antibody-ja50-31-western-blot-6620249631..jpg)
![Western Blot: Glut3 Antibody [NBP3-12237] - WB of NBP3-12237 with rat brain. 1:500 antibody dilution in DiluObuffer. Apparent MW is 45-47 kDa.](http://images.novusbio.com/fullsize/Glut3-Antibody-Western-Blot-NBP3-12237-img0002.jpg "Western Blot: Glut3 Antibody [NBP3-12237] - WB of NBP3-12237 with rat brain. 1:500 antibody dilution in DiluObuffer. Apparent MW is 45-47 kDa.")
![Immunohistochemistry-Paraffin: Glut3 Antibody [NBP3-12237] - Rat testis. 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstrain. 40X magnification. FFPE section.](http://images.novusbio.com/fullsize/Glut3-Antibody-Immunohistochemistry-Paraffin-NBP3-12237-img0004.jpg)
![Western Blot: Glut3 Antibody [NBP3-12237] - Cortical extracts (10 ug total protien/lane) on 10% gel. Primary antibody glucose transporter type 3 diluted 1:2000 in Dilobuffer incubation in primary anitbody 120 minutes at RT; washes 3x10 minutes in TBST secondary antibody alkaline phosphatase conjugated goat anti-rabbit IgG (1:5000)](http://images.novusbio.com/fullsize/Glut3-Antibody-Western-Blot-NBP3-12237-img0001.jpg)
![Immunohistochemistry-Paraffin: Glut3 Antibody [NBP3-12237] - Mouse Spleen-NBP3-12237. 1:100 dilution in IHC blocking buffer. DAB (brown) straining and Hematoxylin QS (blue) counterstrain. 40X magnification. FFPE section](http://images.novusbio.com/fullsize/Glut3-Antibody-Immunohistochemistry-Paraffin-NBP3-12237-img0003.jpg)
![Western Blot: Glut3 Antibody [NBP3-12885] - Unboiled non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glut3 antibody (NBP3-12885) diluted at 1:2000.](http://images.novusbio.com/fullsize/Glut3-Antibody-Western-Blot-NBP3-12885-img0003.jpg "Western Blot: Glut3 Antibody [NBP3-12885] - Unboiled non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glut3 antibody (NBP3-12885) diluted at 1:2000.")
![Immunocytochemistry/Immunofluorescence: Glut3 Antibody [NBP3-12885] - Glut3 antibody detects Glut3 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Glut3 stained by Glut3 antibody (NBP3-12885) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Glut3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-12885-img0004.jpg)
![Western Blot: Glut3 Antibody [NBP3-12885] - Unboiled U87-MG whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glut3 antibody (NBP3-12885) diluted at 1:500. (WCE: whole cell extract; ME: membrane extract)](http://images.novusbio.com/fullsize/Glut3-Antibody-Western-Blot-NBP3-12885-img0001.jpg)
![Western Blot: Glut3 Antibody [NBP3-12885] - Unboiled HepG2 whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glut3 antibody (NBP3-12885) diluted at 1:500. (WCE: whole cell extract; ME: membrane extract)](http://images.novusbio.com/fullsize/Glut3-Antibody-Western-Blot-NBP3-12885-img0002.jpg)
![Immunohistochemistry: Glut3 Antibody (14B1) [NBP3-26317] - Image of Glut3 Antibody (14B1) diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26317_rabbit-glut3-mab-14b1-262202414225217.jpg "Immunohistochemistry: Glut3 Antibody (14B1) [NBP3-26317] - Image of Glut3 Antibody (14B1) diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: Glut3 Antibody (14B1) [NBP3-26317] - Image of Glut3 Antibody (14B1) diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26317_rabbit-glut3-mab-14b1-27220248504613.jpg)
![Immunohistochemistry: Glut3 Antibody (14B1) [NBP3-26317] - Image of Glut3 Antibody (14B1) diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26317_rabbit-glut3-mab-14b1-2722024820393.jpg)
![Flow Cytometry: Glut3 Antibody (14B1) [NBP3-26317] - Overlay Peak curve showing MCF7 cells stained with Glut3 Antibody (14B1) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26317_rabbit-glut3-mab-14b1-282202414312740.jpg)
![Western Blot: Glut3 Antibody (14B1) [NBP3-26317] -Western Blot<br /> Positive WB detected in: 293 whole cell lysate (20µg), JK whole cell lysate (20µg), Hela whole cell lysate (20µg), HepG2 whole cell lysate (20µg), SY5Y whole cell lysate (20µg)<br /> All lanes: SLC2A3 antibody at 1:1000<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 54 kDa<br /> Observed band size: 54 kDa<br />](http://images.novusbio.com/fullsize/nbp3-26317_rabbit-glut3-mab-14b1-29120251248504.jpg)
![Western Blot: Glut3 Antibody [NBP2-36485] - Glut3 - Analysis using 40 ug Human colon cancer tissue lysate at a dilution of 1:400 with Goat anti Rabbit IgG - H&L (HRP) as a secondary antibody at 1:10000 dilution. Exposure time: 2 minutes](http://images.novusbio.com/fullsize/Glut3-Antibody-Western-Blot-NBP2-36485-img0002.jpg "Western Blot: Glut3 Antibody [NBP2-36485] - Glut3 - Analysis using 40 ug Human colon cancer tissue lysate at a dilution of 1:400 with Goat anti Rabbit IgG - H&L (HRP) as a secondary antibody at 1:10000 dilution. Exposure time: 2 minutes")
![Western Blot: Glut3 Antibody (6T3F2) [NBP3-16340] - Western blot analysis of extracts of various cell lines, using Glut3 Rabbit mAb (NBP3-16340) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Glut3-Antibody-6T3F2-Western-Blot-NBP3-16340-img0001.jpg "Western Blot: Glut3 Antibody (6T3F2) [NBP3-16340] - Western blot analysis of extracts of various cell lines, using Glut3 Rabbit mAb (NBP3-16340) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunoprecipitation: Glut3 Antibody (6T3F2) [NBP3-16340] - Immunoprecipitation analysis of 600ug extracts of Mouse brain using 3ug Glut3 antibody (NBP3-16340). Western blot was performed from the immunoprecipitate using Glut3 antibody (NBP3-16340) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Glut3-Antibody-6T3F2-Immunoprecipitation-NBP3-16340-img0002.jpg)
![ELISA: Human Glut3 ELISA Kit (Colorimetric) [NBP2-82171] - Samples were spiked with high concentrations of Human Glut3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-82171_human-glut3-elisa-kit-colorimetric-132202416175670.jpg "ELISA: Human Glut3 ELISA Kit (Colorimetric) [NBP2-82171] - Samples were spiked with high concentrations of Human Glut3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Glut3 ELISA Kit (Colorimetric) [NBP2-82171] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-82171_human-glut3-elisa-kit-colorimetric-432024855010.jpg)
![Western Blot: Glut3 Antibody (S05-5D5) [NBP3-14936] - Western blot detection of Glut3 in Hela, C6, Rat Brain lysates using Glut3 Rabbit mAb (1:500 diluted). Predicted band size: 54kDa. Observed band size: 54kDa.](http://images.novusbio.com/fullsize/Glut3-Antibody-S05-5D5-Western-Blot-NBP3-14936-img0001.jpg "Western Blot: Glut3 Antibody (S05-5D5) [NBP3-14936] - Western blot detection of Glut3 in Hela, C6, Rat Brain lysates using Glut3 Rabbit mAb (1:500 diluted). Predicted band size: 54kDa. Observed band size: 54kDa.")
![ELISA: Mouse Glut3 ELISA Kit (Colorimetric) [NBP2-82172] - Samples were spiked with high concentrations of Mouse Glut3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-82172_mouse-glut3-elisa-kit-colorimetric-132202416121391.jpg "ELISA: Mouse Glut3 ELISA Kit (Colorimetric) [NBP2-82172] - Samples were spiked with high concentrations of Mouse Glut3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Glut3 ELISA Kit (Colorimetric) [NBP2-82172] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-82172_mouse-glut3-elisa-kit-colorimetric-432024915840.jpg)
